Development of optimized novel liposome loaded with 6-gingerol and assessment of its therapeutic activity against NSCLC In vitro and In vivo experimental models

Thangavelu, Priyadharshini; Sundaram, Viswanathan; Gunasekaran, Kaavya; Mujyambere, Bonaventure; Raju, Sowndarya; Kannan, Arya; Arasu, Ashok; Krishna, Kathirvelu; Ramamoorthi, Jayaraj; Sivasamy, R.; Velusamy, Thirunavukkarasu; Ramalingam, Suja

doi:10.1016/j.chemphyslip.2022.105206

Cited by 6 publications

(3 citation statements)

References 62 publications

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“…Furthermore, in our previous study, we observed sustained release of gingerol-loaded DLPC liposomes, consistent with our study [ 39 ]. These findings show that the drug release pattern of liposomal formulations was due to diffusion, partitioning, and dissolution mechanisms [ 40 ].…”

Section: Discussionsupporting

confidence: 93%

“…DAPI staining revealed the morphological alterations of both cancer cell lines compared to the control. In our previous study, cells treated with nanoliposome formulation showed typical apoptotic characteristics of cell fragmentation and shrinkage, and also nucleus showed bright, uniform fluorescent, which indicates chromatin condensation [ 39 ]. The overall DAPI staining results show that apoptosis and cell membrane disintegration was mostly due to the action of dual drug-loaded nanoliposomes exerted on the cell lines.…”

Section: Discussionmentioning

confidence: 99%

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Cytotoxic Effects of Nanoliposomal Cisplatin and Diallyl Disulfide on Breast Cancer and Lung Cancer Cell Lines

et al. 2023

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Dual drug delivery has become the choice of interest nowadays due to its increased therapeutic efficacy in targeting the tumor site precisely. As quoted in recent literature, it has been known to treat several cancers with an acute course of action. Even so, its use is restricted due to the drug’s low pharmacological activity, which leads to poor bioavailability and increases first-pass metabolism. To overcome these issues, a drug delivery system using nanomaterials which would not only encapsulate the drugs of interest but also carry them to the target site of action is needed. Given all these attributes, we have formulated dual drug-loaded nanoliposomes with cisplatin (cis-diamminedichloroplatinum(II) (CDDP)), an effective anti-cancer drug, and diallyl disulfide (DADS), an organosulfur compound derived from garlic. The CDDP and DADS-loaded nanoliposomes (Lipo-CDDP/DADS) exhibited better physical characteristics such as size, zeta potential, polydispersity index, spherical shape, optimal stability, and satisfactory encapsulation percentage. The in vitro anti-cancer activity against MDA-MB-231 and A549 cell lines revealed that Lipo-CDDP/DADS showed significant efficacy against the cancer cell lines, depicted through cell nucleus staining. We conclude that Lipo-CDDP/DADS hold exceptional pharmacological properties with better anti-cancer activity and would serve as a promising formulation to treat various cancers.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Cytotoxic Effects of Nanoliposomal Cisplatin and Diallyl Disulfide on Breast Cancer and Lung Cancer Cell Lines

et al. 2023

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“…Several strategies have been used to increase the bioavailability of these compounds by various researchers [ 24 , 25 ]. For skin delivery of bioactive compounds, lipid-based nanocarriers such as liposomes [ 26 ], nanoemulsions [ 27 ], microemulsions [ 28 ], ethosomes [ 29 ], and solid lipid nanoparticles [ 30 ] have been frequently reported. The delivery of active chemical compounds through the barriers of the skin, namely the stratum corneum, is the most difficult aspect of developing drug delivery [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

QbD-Optimized, Phospholipid-Based Elastic Nanovesicles for the Effective Delivery of 6-Gingerol: A Promising Topical Option for Pain-Related Disorders

Ghazwani

Alqarni

Hani

et al. 2023

IJMS

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In this study, elastic nanovesicles, constructed of phospholipids optimized by Quality by Design (QbD), release 6-gingerol (6-G), a natural chemical that may alleviate osteoporosis and musculoskeletal-related pain. A 6-gingerol-loaded transfersome (6-GTF) formulation was developed using a thin film and sonication approach. 6-GTFs were optimized using BBD. Vesicle size, PDI, zeta potential, TEM, in vitro drug release, and antioxidant activity were evaluated for the 6-GTF formulation. The optimized 6-GTF formulation had a 160.42 nm vesicle size, a 0.259 PDI, and a −32.12 mV zeta potential. TEM showed sphericity. The 6-GTF formulation’s in vitro drug release was 69.21%, compared to 47.71% for the pure drug suspension. The Higuchi model best described 6-G release from transfersomes, while the Korsmeyer–Peppas model supported non-Fickian diffusion. 6-GTF had more antioxidant activity than the pure 6-G suspension. The optimized transfersome formulation was converted into a gel to improve skin retention and efficacy. The optimized gel had a spreadability of 13.46 ± 4.42 g·cm/s and an extrudability of 15.19 ± 2.01 g/cm2. The suspension gel had a 1.5 μg/cm2/h ex vivo skin penetration flux, while the 6-GTF gel had 2.71 μg/cm2/h. Rhodamine B-loaded TF gel reached deeper skin layers (25 μm) compared to the control solution in the CLSM study. The gel formulation’s pH, drug concentration, and texture were assessed. This study developed QbD-optimized 6-gingerol-loaded transfersomes. 6-GTF gel improved skin absorption, drug release, and antioxidant activity. These results show that the 6-GTF gel formulation has the ability to treat pain-related illnesses effectively. Hence, this study offers a possible topical treatment for conditions connected to pain.

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Nano-mediated strategy for targeting and treatment of non-small cell lung cancer (NSCLC)

Ashique¹,

Garg²,

Mishra

et al. 2023

Naunyn-Schmiedeberg's Arch Pharmacol

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Development of optimized novel liposome loaded with 6-gingerol and assessment of its therapeutic activity against NSCLC In vitro and In vivo experimental models

Cited by 6 publications

References 62 publications

Cytotoxic Effects of Nanoliposomal Cisplatin and Diallyl Disulfide on Breast Cancer and Lung Cancer Cell Lines

Cytotoxic Effects of Nanoliposomal Cisplatin and Diallyl Disulfide on Breast Cancer and Lung Cancer Cell Lines

QbD-Optimized, Phospholipid-Based Elastic Nanovesicles for the Effective Delivery of 6-Gingerol: A Promising Topical Option for Pain-Related Disorders

Nano-mediated strategy for targeting and treatment of non-small cell lung cancer (NSCLC)

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