Development of PCR-based markers for the identification and detection of Lophodermella needle cast pathogens on Pinus contorta var. latifolia and P. flexilis

Ata, Jessa P.; Burns, Kelly S.; Marchetti, Suzanne B.; Worrall, James J.; Mondo, Stephen J.; Stewart, Jane E.

doi:10.1016/j.mimet.2022.106546

Cited by 1 publication

(4 citation statements)

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“…linearis and Lophodermium spp. have been detected on asymptomatic needles using molecular techniques (Ata, Burns, et al., 2022; Reignoux et al., 2014; Stenström & Ihrmark, 2005), and Coccomyces strobi J. Reid & Cain and Tryblidiopsis magnesii Tanney & Seifert have also been characterized as endophytes (McMullin et al., 2019; Tanney & Seifert, 2019). It was further revealed that some Rhytismataceae species (i.e., L .…”

Section: Ecology and Epidemiologymentioning

confidence: 99%

“…linearis on P . flexilis (Ata, Burns, et al., 2022). The species‐specific primers also detected the target pathogens in asymptomatic P .…”

Section: Disease Diagnosis and Managementmentioning

confidence: 99%

“…While promising for natural control of needle diseases, occurrences of saprophytic species tend to be restricted to areas with high humidity (Bingzhang et al, 1994;Millar, 1984) and others like H. pinicola may become primary pathogens (Stahl et al, 1988). (Ata, Burns, et al, 2022).…”

Section: Speciesmentioning

confidence: 99%

“…on asymptomatic needles using molecular techniques (Ata, Burns, et al, 2022;Reignoux et al, 2014;Stenström & Ihrmark, 2005) Rhytismataceae needle pathogens, like many foliar pathogens, prefer warm moist conditions such as wet springs and early summers, and/or humid areas such as thick undergrowth and those at high elevation. Such favourable conditions must coincide with spore release and availability of susceptible foliage to facilitate disease development (Scharpf, 1989;Worrall & Sullivan, 2002).…”

Section: Speciesmentioning

confidence: 99%

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Needle pathogens of Rhytismataceae: Current knowledge and research opportunities for conifer foliar diseases

Ata,

Burns,

Stewart

2024

Forest Pathology

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Needle diseases caused by pathogens within Rhytismataceae are increasing in frequency globally where pines are either a predominate species or are grown commercially, and yet remain poorly understood. Here, we provide background information on the current knowledge and management strategies of needle pathogens in the Rhytismataceae and discuss research opportunities using molecular tools to fill knowledge gaps. Many of the aggressive needle pathogens within this group lack morphological features to delimit species and are difficult to grow in axenic cultures, which complicates scientific investigations. Molecular research has greatly advanced knowledge of this unique group of pathogens. Nucleotide sequences from multiple gene loci identified greater diversity within this group of pathogens than previously thought, as new and cryptic species are being discovered in broader geographic regions. High throughput sequencing and analyses revealed that an endophytic lifestyle is a common trait among fastidious if not obligate needle pathogens within Rhytismataceae. Further, molecular analyses showed the impact these pathogens have on the diversity of endophytic microbiota is underestimated. Early detection in healthy‐looking needles using PCR‐based markers greatly improves capabilities for disease diagnosis and monitoring. Despite growing number of molecular studies on Rhytismataceae needle pathogens, fundamental questions on the taxonomy, phylogeny, ecology and pathology of many Rhytismataceae needle pathogens remain unanswered. Nonetheless, with the projected changes in the occurrence and severity of Rhytismataceae pathogens, increased accessibility to molecular tools vastly expands the potential to gain more insights into understudied needle pathosystems to help define and address its impact under changing environments and consequently inform forest disease management more effectively.

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