Development of personalized cell-populated vascular graft in vitro

Khanova, Mariam Yu.; Великанова, Е. А.; Глушкова, Т. В.; Матвеева, В. Г.

doi:10.17802/2306-1278-2021-10-2s-89-93

Cited by 3 publications

(1 citation statement)

References 8 publications

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“…In addition, a promising scientific direction is the creation of vascular prostheses using tissue engineering methods [ 5 ]. This makes it possible to produce matrices with predetermined properties: structural stability and controlled biodegradation, recruitment of cells to colonize the prosthesis surface, and low immunogenicity [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

New Tissue-Engineered Vascular Matrix Based on Regenerated Silk Fibroin: in vitro Study

Prokudina,

Senokosova,

Antonova

et al. 2023

Sovrem Tehnol Med

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The aim of the study was to make a vascular patch based on regenerated silk fibroin (SF) and study its physical and mechanical characteristics, biocompatibility and matrix properties in comparison with polyhydroxybutyrate/valerate/polycaprolactone with incorporated vascular endothelial growth factor (PHBV/PCL/VEGF) and commercial bovine xenopericardium (XP) flap in experiments in vitro . Materials and Methods Tissue-engineered matrices were produced by electrospinning. The surface structure, physical and mechanical characteristics, hemocompatibility (erythrocyte hemolysis, aggregation, adhesion and activation of platelets after contact with the material) and matrix properties of vascular patches (adhesion, viability, metabolic activity of EA.hy926 cells on the material) were studied. Results The surface of SF-based matrices and PHBV/PCL/VEGF-based tissue engineered patches had a porous and fibrous structure compared to a denser and more uniform XP flap. The physical and mechanical characteristics of SF matrices were close to those of native vessels. Along with this, tissue-engineered patches demonstrated high hemocompatible properties, which do not differ from those for commercial XP flap. Adhesion, viability, and metabolic activity of EA.hy926 endothelial cells also corresponded to the previously developed PHBV/PCL/VEGF matrix and XP flap, which indicates the nontoxicity and biocompatibility of SF matrices. Conclusion Matrices produced from regenerated SF demonstrated satisfactory results, comparable to those for PHBV/PCL/VEGF and commercial XP flap, and in the case of platelet adhesion and activation, they outperformed these patches. In total, SF can be defined as material having sufficient biological compatibility, which makes it possible to consider a tissue-engineered matrix made from it as promising for implantation into the vascular wall.

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Section: Introductionmentioning

confidence: 99%

New Tissue-Engineered Vascular Matrix Based on Regenerated Silk Fibroin: in vitro Study

Prokudina,

Senokosova,

Antonova

et al. 2023

Sovrem Tehnol Med

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The first results of obtaining a hybrid hydrogel based on fibrin and polyvinyl alcohol: comparison with monocomponent hydrogels

Senokosova

Резвова

Sevost'yanova

et al. 2023

SJCEM

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Fibrin displays promising characteristics for tissue engineering. However, it has suboptimal physical and mechanical properties when used as a material for cardiovascular applications. Obtaining an interpenetrating polymer network (IPN) hydrogel based on fibrin and polyvinyl alcohol (PVA) can improve the physical and mechanical characteristics and shrink behavior of fibrin.Aim: To perform sequential polymerization of fibrin and PVA to obtain IPN hydrogel and analyze its properties in comparison with monocomponent hydrogels.Material and Methods. Fibrinogen was isolated from the peripheral blood of patients using ethanol precipitation, then polyvinyl alcohol dissolved in saline was added to it. First, fibrin polymerization was initiated by adding calcium chloride to the solution. Then, it was followed by polyvinyl alcohol undergoing freeze–thaw cycles. Thus, a hydrogel based on fibrin and PVA, samples from pure fibrin and pure polyvinyl alcohol were prepared. We studied the structure of hydrogels, their physical and mechanical properties, shrink behavior and biological activity. Statistical data processing was carried out using the GraphPad Prism 6 software.Results. 3D structure of the hydrogel is presented by polyvinyl alcohol polygonal cavities with a network of thin fibrin fibers. The distribution of components was equal in the inside of the samples, while polyvinyl alcohol prevails on the surface. Elongation (247 (220.0; 293.2)%; p = 0.0005) and Young’s modulus (0.09 (0.11; 0.13) mPa; p = 0.0001) of the hydrogel were statistically significantly higher compared to fibrin values. The hydrogel did not shrink, unlike fibrin that shrunk (11-fold decrease in volume). The number of adherent endothelial cells on the hydrogel matrices was 8 times higher than on polyvinyl alcohol, but 10 times lower than on fibrin. There was no proliferative activity of cells on polyvinyl alcohol, but 13.6% of proliferating cells were noted on the IPN hydrogel, and 59.52% on fibrinConclusion. Using sequential polymerization to obtain the IPN hydrogel based on fibrin and polyvinyl alcohol provides an equal distribution of fibers in the thickness of the material, making it possible to obtain hydrogels with improved mechanical properties and shrink behavior. However, the components on the surface of the IPN hydrogel need to be redistributed - more polyvinyl alcohol should be added still maintaining a relatively low adhesiveness of the material. Therefore further research is necessary to create the most optimal conditions for cell activity.

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Analysis of the effectiveness of various protein coatings for optimizing the endothelialization of polymer matrices

Великанова

Матвеева

Senokosova

et al. 2023

SJCEM

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Background. Due to the constant increase in the number of surgeries to restore blood flow in the affected vessels, the development of synthetic prostheses is relevant. One of the key success factors is an increase in the adhesive properties of the inner surface, since the rapid endothelialization of vascular prostheses is considered a factor necessary to prevent thrombosis and neointimal hypertrophy.Aim: To determine the effect of surface modification of polymer matrices with fibrin, fibronectin, or type I collagen on the adhesion and viability of endothelial cells.Material and Methods. Polymer matrices prepared by electrospinning from a mixture of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and poly(ε-caprolactone) were studied. Matrix samples were coated with type I collagen or fibronectin or fibrin. Then, human umbilical vein endothelial cells (HUVEC) were colonized on the matrices and cultured for 3 days. Unmodified matrices and culture plate wells were used as controls. Cell viability was assessed by combined staining with Hoechst 33342 and ethidium bromide. The metabolic activity of the cells was studied using the MTT test. Cell adhesion was analyzed by staining for F-actin. Statistical analysis of the results was performed using the GraphPrism 7.0 program.Results. It was found that the number of adherent cells and their metabolic activity of matrices with collagen did not differ from unmodified ones. Coating with fibronectin demonstrated higher rates of cell adhesion to the surface. However, a rather high level of cell death in this group indicates that such a modification cannot fully ensure the normal functioning of cells. Finally, we observed the best results when using a fibrin coating, which was comparable to culture plastic in terms of adhesion and viability of endothelial cells.Conclusion. Modification of the surface of polymer matrices with fibrin can significantly improve their adhesive properties and can be used in the development of polymer prostheses for small-diameter vessels.

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Development of personalized cell-populated vascular graft in vitro

Cited by 3 publications

References 8 publications

New Tissue-Engineered Vascular Matrix Based on Regenerated Silk Fibroin: in vitro Study

New Tissue-Engineered Vascular Matrix Based on Regenerated Silk Fibroin: in vitro Study

The first results of obtaining a hybrid hydrogel based on fibrin and polyvinyl alcohol: comparison with monocomponent hydrogels

Analysis of the effectiveness of various protein coatings for optimizing the endothelialization of polymer matrices

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