Development of Phagoindication Method for Pseudomonas Syringae Bacteria in Sanitary Control Objects

Abstract

The article presents the results of research on the development of parameters for practical application of phage biopreparation Pseudomonas syringae in order to indicate the data of phytopathogenic bacteria in sanitary control objects. The introduction of the article describes pathogens of Pseudomonas syringae and plants that they affect, which proves research actuality aimed at developing speed-up methods of indication and identification of bacteria-pathogens, which will allow specialists to develop measures to combat above-mentioned phytopathogens in a short time. It was established that cultivation of the "bacteriophage-test material" system at a temperature of 28 ± 1 0C during 3.5 hours allows detecting Pseudomonas syringae bacteria in soil samples, river water, and cucumber seeds by the RSF method at a concentration of 103 MK/ml., increase in the exposure time does not improve the quality of the reaction. The bacteriophages Ps. s-7 UlGAU and Ps. s-27 UlGAU in monoculture were used in experiments at a concentration of 103 BFU/ml. When testing a polyphage biological product in RSF, including all the above-mentioned bacteriophages, it was found that the results obtained in the studies of the river water sample were similar to the experimental data on cucumber seed sample. Concentration of detected Pseudomonas syringae bacteria was 103 m.k./ml. It was experimentally established that reducing the initial titer of polyphage biopreparation to a concentration of 102 BFU / ml allowed for the indication of Pseudomonas syringae bacteria in a soil sample at a concentration of 103 mk/g.