Development of photostabilized asymmetrical cyanine dyes for<i>in vivo</i>photoacoustic imaging of tumors

Onoe, Satoru; Temma, Takashi; Kanazaki, Kengo; Ono, Masahiro; Saji, Hideo

doi:10.1117/1.jbo.20.9.096006

Cited by 18 publications

(8 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…to later develop asymmetrical derivatives of 8 functionalized with a 4‐nitrobenzyl alcohol group to serve as a triplet quencher moiety (Fig. 9 – 11 ) . It was found that these triplet quencher functionalized derivatives displayed in vitro PA signals that were equivalent to 8 while showing a 1.5‐fold increase in photostability.…”

Section: Molecular Photoacoustic Contrast Agentsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Photoacoustic Contrast Agents: Design Principles & Applications

Borg

Rochford

2018

Photochem & Photobiology

View full text Add to dashboard Cite

Photoacoustic imaging (PAI) is a rapidly growing field which offers high spatial resolution and high contrast for deep-tissue imaging in vivo. PAI is nonionizing and noninvasive and combines the optical resolution of fluorescence imaging with the spatial resolution of ultrasound imaging. In particular, the development of exogenous PA contrast agents has gained significant momentum of late with a vastly expanding complexity of dye materials under investigation ranging from small molecules to macromolecular proteins, polymeric and inorganic nanoparticles. The goal of this review is to survey the current state of the art in molecular photoacoustic contrast agents (MPACs) for applications in biomedical imaging. The fundamental design principles of MPACs are presented and a review of prior reports spanning from early-to-current literature is put forth.

show abstract

Section: Molecular Photoacoustic Contrast Agentsmentioning

confidence: 99%

“…Molecular structures of the asymmetrical cyanine derivatives 9 – 11 reported by Onoe et al . with enhanced photostability relative to ICG ( 4 ) and 8 .…”

Section: Molecular Photoacoustic Contrast Agentsmentioning

confidence: 99%

Molecular Photoacoustic Contrast Agents: Design Principles & Applications

Borg

Rochford

2018

Photochem & Photobiology

View full text Add to dashboard Cite

show abstract

“…Among them, PA imaging, which detects ultrasound thermoelastically generated from the optical absorbers irradiated with pulsed laser, has emerged as an attractive modality due to sensitive and high resolution imaging at a depth of several centimeters in vivo 2 3 . Currently, various PA signal-enhancing contrast agents (PA probes), including metallic nanoparticles 4 5 and fluorescence dyes 6 7 8 have been reported to aid in the diagnosis of tumors. However, these probes still remain in pre-clinical and clinical trials and are not in clinical use.…”

mentioning

confidence: 99%

Polyoxazoline multivalently conjugated with indocyanine green for sensitive in vivo photoacoustic imaging of tumors

Kanazaki

Sano

Makino

et al. 2016

Sci Rep

Self Cite

View full text Add to dashboard Cite

Photoacoustic imaging, which enables high-resolution imaging in deep tissues, has lately attracted considerable attention. For tumor imaging, photoacoustic probes have been proposed to enhance the photoacoustic effect to improve detection sensitivity. Here, we evaluated the feasibility of using a biocompatible hydrophilic polymer, polyoxazoline, conjugated with indocyanine green (ICG) as a tumor-targeted photoacoustic probe via enhanced permeability and retention effect. ICG molecules were multivalently conjugated to partially hydrolyzed polyoxazoline, thereby serving as highly sensitive photoacoustic probes. Interestingly, loading multiple ICG molecules to polyoxazoline significantly enhanced photoacoustic signal intensity under the same ICG concentration. In vivo biodistribution studies using tumor bearing mice demonstrated that 5% hydrolyzed polyoxazoline (50 kDa) conjugated with ICG (ICG/polyoxazoline = 7.8), P14-ICG7.8, showed relatively high tumor accumulation (9.4%ID/g), resulting in delivery of the highest dose of ICG among the probes tested. P14-ICG7.8 enabled clear visualization of the tumor regions by photoacoustic imaging 24 h after administration; the photoacoustic signal increased in proportion with the injected dose. In addition, the signal intensity in blood vessels in the photoacoustic images did not show much change, which was attributed to the high tumor-to-blood ratios of P14-ICG7.8. These results suggest that polyoxazoline-ICG would serve as a robust probe for sensitive photoacoustic tumor imaging.

show abstract

“…In another example, Onoe et al . developed a symmetrical NIR cyanine dye for cancer imaging utilizing fluorescence and photoacoustic imaging 20 , but this probe degraded under high-energy laser pulses during imaging 21 .…”

Section: Introductionmentioning

confidence: 99%

Molecular imaging of oxidative stress using an LED-based photoacoustic imaging system

Hariri¹,

Zhao²,

Jeevarathinam³

et al. 2019

Sci Rep

View full text Add to dashboard Cite

LED-based photoacoustic imaging has practical value in that it is affordable and rugged; however, this technology has largely been confined to anatomic imaging with limited applications into functional or molecular imaging. Here, we report molecular imaging reactive oxygen and nitrogen species (RONS) with a near-infrared (NIR) absorbing small molecule (CyBA) and LED-based photoacoustic imaging equipment. CyBA produces increasing photoacoustic signal in response to peroxynitrite (ONOO − ) and hydrogen peroxide (H 2 O 2 ) with photoacoustic signal increases of 3.54 and 4.23-fold at 50 µM of RONS at 700 nm, respectively. CyBA is insensitive to OCl − , ˙NO, NO 2 − , NO 3 − , tBuOOH, O 2 − , C 4 H 9 O˙, HNO, and ˙OH, but can detect ONOO − in whole blood and plasma. CyBA was then used to detect endogenous RONS in macrophage RAW 246.7 cells as well as a rodent model; these results were confirmed with fluorescence microscopy. Importantly, CyB suffers photobleaching under a Nd:YAG laser but the signal decrease is <2% with the low-power LED-based photoacoustic system and the same radiant exposure time. To the best of our knowledge, this is the first report to describe molecular imaging with an LED-based photoacoustic scanner. This study not only reveals the sensitive photoacoustic detection of RONS but also highlights the utility of LED-based photoacoustic imaging.

show abstract

Development of photostabilized asymmetrical cyanine dyes forin vivophotoacoustic imaging of tumors

Cited by 18 publications

References 30 publications

Molecular Photoacoustic Contrast Agents: Design Principles & Applications

Molecular Photoacoustic Contrast Agents: Design Principles & Applications

Polyoxazoline multivalently conjugated with indocyanine green for sensitive in vivo photoacoustic imaging of tumors

Molecular imaging of oxidative stress using an LED-based photoacoustic imaging system

Contact Info

Product

Resources

About