Development of Plant-produced E2 Protein for Use as a Green Vaccine Against Classical Swine Fever Virus

Sohn, Eun‐Ju; Lee, Yong-Jik; Park, Namjo; Park, Min Hee; Kim, Nam Hyung; Park, Soohong; Min, Kyungmin; Gu, Sungmin; Park, Young-Min; Song, Jae‐Young; An, Dong-Jun; Hwang, Inhwan

doi:10.1007/s12374-018-0133-4

Cited by 21 publications

(26 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A previous study found that the endoplasmic reticulum (ER) accumulates high levels of recombinant proteins (Gomord et al, 1997;Kang et al, 2018;Sohn et al, 2018;Wandelt et al, 1992). Thus, to achieve a high level of expression, we used the BiP leader sequence to target the recombinant protein to the ER.…”

Section: Design Of a Recombinant Gene For High Level Expression In Plmentioning

confidence: 99%

“…The most powerful is transient expression following Agrobacterium-mediated gene transfer into host plants via RNA virus-mediated RNA amplification or DNA virus-based gene amplification. Another important approach is chloroplast transformation, which integrates the target gene into the chloroplast genome and thereby leads to extremely high levels of protein production (Staub et al, 2000;Verma and Daniell, 2007;Zhang et al, 2017); the transgenic approach, which integrates foreign genes into the plant nuclear genome, is also widely used (Lee et al, 2015;Sohn et al, 2018;Twyman et al, 2002). Many different strategies using transgenic plants can be employed as recombinant proteins can be obtained from either whole plants or cell cultures (Holtz et al, 2015; Rosales-Mendoza and Nieto-G omez, 2018;Tekoah et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, cellulose-binding domains (CBDs), which have a high binding affinity to cellulose, an inexpensive biomaterial, have been used as affinity tags (Bayer et al, 1998;Sohn et al, 2018;Wan et al, 2011;You and Zhang, 2013). The CBDs can be divided into multiple groups and show specific binding to either the crystalline or amorphous regions of cellulose fibres.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cost‐effective production of tag‐less recombinant protein in Nicotiana benthamiana

Islam

Kwak

Lee

et al. 2018

Plant Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

Summary Plants have recently received a great deal of attention as a means of producing recombinant proteins. Despite this, a limited number of recombinant proteins are currently on the market and, if plants are to be more widely used, a cost‐effective and efficient purification method is urgently needed. Although affinity tags are convenient tools for protein purification, the presence of a tag on the recombinant protein is undesirable for many applications. A cost‐effective method of purification using an affinity tag and the removal of the tag after purification has been developed. The family 3 cellulose‐binding domain ( CBM 3), which binds to microcrystalline cellulose, served as the affinity tag and the small ubiquitin‐related modifier ( SUMO ) and SUMO ‐specific protease were used to remove it. This method, together with size‐exclusion chromatography, enabled purification of human interleukin‐6 ( hIL 6) with a yield of 18.49 mg/kg fresh weight from leaf extracts of Nicotiana benthamiana following Agrobacterium ‐mediated transient expression. Plant‐produced hIL 6 (P‐ hIL 6) contained less than 0.2 EU/μg (0.02 ng/mL) endotoxin. P‐ hIL 6 activated the Janus kinase‐signal transducer and activator of transcriptional pathways in human LNC aP cells, and induced expression of IL ‐21 in activated mouse CD 4 + T cells. This approach is thus a powerful method for producing recombinant proteins in plants.

show abstract

Section: Design Of a Recombinant Gene For High Level Expression In Plmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cost‐effective production of tag‐less recombinant protein in Nicotiana benthamiana

Islam

Kwak

Lee

et al. 2018

Plant Biotechnology Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…Arabidopsis thaliana is a low‐cost model organism amenable to several areas of research of pressing global importance. These research areas include climate change (Ahuja, de Vos, Bones, & Hall, ; Hancock et al., ; Li, Huang, Bergelson, Nordborg, & Borevitz, ; Samis et al., ; Wilczek, Cooper, Korves, & Schmitt, ), food security (Blümel, Dally, & Jung, ; Chardon, Noël, & Masclaux‐Daubresse, ; Chew & Halliday, ; Lenk, Wenig, Mengel, Häußler, & Vlot, ; Naqvi et al., ; O'Conner et al., ), biofuel production (Lee, Mo, Kim, & Chapple, ; Vargas et al., ), and vaccine development (Kalbina, Engstrand, Andersson, & Strid, ; Sohn et al., ).…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis Thaliana: From Weed to Model Organism

Diaz¹

2019

CP Essential Lab Tech

View full text Add to dashboard Cite

Arabidopsis thaliana is a small plant of significant economic and agronomic importance. While Arabidopsis is not a crop plant, it is not so different in its fundamental properties. Arabidopsis is a member of the Brassicaceae family, which constitutes one of the world's most economically important plant groups. According to the United Nations, globally Brassicaceae crops are worth $31 billion, and the number is likely to increase since a number of related species within this family are underutilized edible varieties. Its small size is an advantage to researchers with limited space and funding; simply put, a smaller plant requires fewer resources. Arabidopsis has been studied most intensely for the last 40 years and officially became a model plant in the late 1990s. Since then, the community has developed genetic and genomic resources so numerous that the barrier to entry to studying Arabidopsis is relatively low. This article provides a primer to how Arabidopsis came to be a model organism and highlights essential techniques every Arabidopsis researcher should be aware of to advance the pace of discovery. © 2019 by John Wiley & Sons, Inc.

show abstract

“…It is noteworthy that biochemical, structural, and functional studies of plant ATG proteins are severely hampered by the difficulty in protein production, a challenge often encountered when working in plant proteins in general [24]. There exists several technologies dedicated to producing proteins in plants, including the Agrobacterium-mediated transient gene expression [24], chloroplast transformation [25], and stable transformation that integrates foreign genes into the plant nuclear genome [26]. Unfortunately, both the transient expression and the chloroplast transformation are of only limited use at present owing to the inability to either express proteins of large size or in sufficient quantity, which is particularly important for structural studies.…”

mentioning

confidence: 99%

Structural Biology and Electron Microscopy of the Autophagy Molecular Machinery

Lai

Zhang

et al. 2019

Cells

View full text Add to dashboard Cite

Autophagy is a highly regulated bulk degradation process that plays a key role in the maintenance of cellular homeostasis. During autophagy, a double membrane-bound compartment termed the autophagosome is formed through de novo nucleation and assembly of membrane sources to engulf unwanted cytoplasmic components and targets them to the lysosome or vacuole for degradation. Central to this process are the autophagy-related (ATG) proteins, which play a critical role in plant fitness, immunity, and environmental stress response. Over the past few years, cryo-electron microscopy (cryo-EM) and single-particle analysis has matured into a powerful and versatile technique for the structural determination of protein complexes at high resolution and has contributed greatly to our current understanding of the molecular mechanisms underlying autophagosome biogenesis. Here we describe the plant-specific ATG proteins and summarize recent structural and mechanistic studies on the protein machinery involved in autophagy initiation with an emphasis on those by single-particle analysis.

show abstract

Development of Plant-produced E2 Protein for Use as a Green Vaccine Against Classical Swine Fever Virus

Cited by 21 publications

References 58 publications

Cost‐effective production of tag‐less recombinant protein in Nicotiana benthamiana

Cost‐effective production of tag‐less recombinant protein in Nicotiana benthamiana

Arabidopsis Thaliana: From Weed to Model Organism

Structural Biology and Electron Microscopy of the Autophagy Molecular Machinery

Contact Info

Product

Resources

About