Development of Plant Protein Derived Tri Angular Shaped Nano Zinc Oxide Particles with Inherent Antibacterial and Neurotoxicity Properties

Abstract

The synthesis of nanometer-sized metallic nanoparticles utilizing bio-sources is one of the most cost-effective and ecologically friendly approaches. Nano-zinc oxide particles (N-ZnO Ps) were made using a simple green synthesis method using an aqueous zinc nitrate salt and Perilla frutescens crude protein as a protecting and reducing agent in the current work. UV-visible (UV-vis) spectrophotometry, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), (energy dispersive x-ray spectroscopy) EDX and high-resolution transmission electron microscopy (HR-TEM) were used to characterize the synthesized N-ZnO Ps. A distinctive UV-vis absorption peak was observed at 370 nm due to N-ZnO Ps. The SEM and HR-TEM pictures revealed N-ZnO Ps with a triangular form. The XRD pattern indicated the wurtzite structure of N-ZnO Ps. Nanoparticles exhibited a zeta potential of −11.3 mV. The antibacterial activity of N-ZnO Ps was tested against Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumonia) microorganisms. The N-ZnO Ps were non-toxic to HMC-3 human normal brain microglia cells; however, they exhibited a potential cytotoxic effect on the LN-18 human brain glioblastoma cell line. These results indicate that N-ZnOPs can act as promising antibacterial and anticancer treatments in the prevention of Glioblastoma.

Emerging Strategies to Improve the Design and Manufacturing of Biocompatible Therapeutic Materials

“…Hou et al [ 1 ] developed nano-zinc-oxide particles as an example of nanometer-sized metallic entities exhibiting antibacterial and neurotoxicity behavior. They were tested against Escherichia coli ( E. coli ) and Klebsiella pneumoniae ( K. pneumonia ) microorganisms; additional nontoxic effects toward HMC-3 human normal brain microglia cells were reported, along with a potential cytotoxic effect on the LN-18 human brain glioblastoma cell line.…”

Antibacterial and Photodegradation of Organic Dyes Using Lamiaceae-Mediated ZnO Nanoparticles: A Review

“…However, the authors did not report on the phytochemical compounds present in the methanoic extracts. Hou et al [78] extracted proteins from Perilla frutescens for ZnO NPs synthesis using petroleum ether. The choice of solvent for the extraction of biomolecules from plants is dependent on the targeted biomolecules.…”

Plant-Derived Exosome-Like Nanoparticles: Emerging Nanosystems for Enhanced Tissue Engineering

“…Plant-based nanomaterials have emerged as highly compelling candidates within diverse biocompatible systems, principally due to their exceptional attributes. 5 , 6 Foremost among these attributes is the innate biocompatibility inherent in plant-derived materials. This quality plays a crucial role in mitigating the tendency for immunogenic responses and toxic manifestations, commonly observed with synthetic analogs.…”

“…This intrinsic bioactivity sets them apart from relatively inert biomaterials like Polyvinyl Alcohol (PVA) or Poly(ε-caprolactone) (PCL). 5 , 10 Moreover, plant-derived ELNs demonstrate high biocompatibility and low immunogenicity, a crucial advantage over materials such as Ultra-High Molecular Weight Polyethylene (UHMWPE) that may elicit undesirable immunogenic responses. 6 , 11 A significant strength is the precision of targeting and drug delivery facilitated by these ELNs, as they can be engineered to encapsulate therapeutic cargo with a natural affinity to specific cell types.…”