Development of Polymerase Chain Reaction and Comparison with In situ Hybridization for the Detection of Haemophilus parasuis in Formalin-Fixed, Paraffin-Embedded Tissues

Jung, Kwonil; Ha, Yoon‐Cheol; Kim, Sung Hoon; Chae, Chanhee

doi:10.1292/jvms.66.841

Cited by 11 publications

(5 citation statements)

References 12 publications

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“…Tissue biopsy preparation. The procedure for preparation of the tissue biopsies was adopted as described elsewhere [Shi et al, 2002;Jung et al, 2004;Keohavong et al, 2004]. The formalin fixed paraffin embedded (FFPE) tissue blocks were melted on a hot plate and cut in to small pieces of the tissue, 1-2 mm.…”

Section: Laboratory Testsmentioning

confidence: 99%

Presence ofBetapapillomavirusin Kaposi sarcoma lesions

et al. 2014

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Human herpes virus 8 (HHV 8) is recognized as the necessary cause of Kaposi sarcoma (KS) and in the recent past the human papillomavirus (HPV) has been linked to the development of cutaneous basal cell and squamous cell carcinomas. In a cross sectional study investigating Beta-HPV infections in skin lesions, an unexpected occurrence of HPV DNA was found in KS lesions of HIV infected individuals. Of the 18 KS cases included in the study 16 (89%) had HPV DNA detected. The most common Betapapillomavirus types were HPV14 [15 cases (83.3%)], HPV12 [8 cases (44.4%)], and HPV24 [7 cases (39%)]. Multiple Beta-HPV types were detected in 10 (62.5%) of the participants with HPV DNA positive lesions; of these 7 had a CD4+ count below 350 cells/µl and 3 had CD4+ counts above 350 cells/µl. The presence of Beta-HPV DNA in KS lesions is a newly described phenomenon. Further studies to elucidate the role of Beta-HPV in KS need to be conducted as it is possible that HHV 8 may not be the solitary viral carcinogen in KS tumorigenesis.

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Section: Laboratory Testsmentioning

confidence: 99%

Presence ofBetapapillomavirusin Kaposi sarcoma lesions

et al. 2014

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“…For this reason, other alternative tests have been reported for improving diagnosis of Glässer’s disease, such as several molecular methods (Oliveira et al. 2001; Jung et al. 2004; Angen et al.…”

Section: Introductionmentioning

confidence: 99%

“…This organism depends on an external source of nicotinamide adenine dinucleotide (NAD) for growth, and this fastidious nature impairs its recovery from clinical samples, especially from those being more contaminated (Pijoan et al 1983). For this reason, other alternative tests have been reported for improving diagnosis of Glässer's disease, such as several molecular methods (Oliveira et al 2001;Jung et al 2004;Angen et al 2007;Chen et al 2010) and, among them, a real-time PCR (RT-PCR) based on the amplification of the infB gene (Turni et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Comparison of real-time PCR and culture isolation in colostrum-deprived pigs immunized and challenged with Haemophilus parasuis

Frandoloso

Martínez-Martínez

Ferri

et al. 2011

Letters in Applied Microbiology

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Aims: A real‐time PCR (RT‐PCR) based on the detection of the infB gene of Haemophilus parasuis is compared with culture isolation (Frandoloso et al., (2011) Clin Vaccine Immunol18, 50–58.), evaluating different subunit or commercial vaccines. Methods and Results: Samples from different tissues of 24 experimentally infected and challenged colostrum‐deprived piglets were tested. The RT‐PCR gave globally a 23·3% more of positive results than culture, and all samples being positive by culture were positive by RT‐PCR also. H. parasuis could not be cultured from any of the samples of the piglets included in the three vaccinated groups resulting in a strong protection, but it could be detected by RT‐PCR in six samples in the group immunized with the commercial vaccine, in three in that vaccinated with native proteins with affinity to porcine transferrin (NPAPT) administered intramuscularly and in only two in that immunized with NPAPT intratracheally. Conclusions: The RT‐PCR was more sensitive than culture for H. parasuis detection in the organs compared. Significance and Impact of the Study: The RT‐PCR evidenced that NPAPT vaccines were those yielding the best protection results in terms of H. parasuis clearance.

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“…O agente pode ser também identificado nos tecidos hepáticos, pericárdio, pulmão e meninge utilizando as técnicas de imunohistoquímica (IHQ) ou a hibridização in situ (IHS). Entretanto, a especificidade da IHS não foi totalmente esclarecida e alguns testes de IHQ apresentaram reação cruzada com Actinobacillus pleuropneumoniae (Segalés et al, 1997;Jung et al, 2004).…”

Section: Diagnósticounclassified

Doença de Glässer: Uma revisão

2019

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Haemophilus parasuis é o agente etiológico da doença de Glässer e causa graves prejuízos econômicos na indústria suína. O diagnóstico da doença é baseado na associação do histórico clínico do rebanho, isolamento da bactéria e sorotipagem. O difícil controle do agente e das co-infecções tornam-se imprescindível o uso de tratamentos estratégicos e programas vacinais, conjugados com boas práticas de manejo. Esta revisão apresenta uma abordagem geral da Doença de Glässer e discute os aspectos epidemiológicos, diagnóstico, imunidade, prevenção e controle.

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Development of Polymerase Chain Reaction and Comparison with In situ Hybridization for the Detection of Haemophilus parasuis in Formalin-Fixed, Paraffin-Embedded Tissues

Cited by 11 publications

References 12 publications

Presence ofBetapapillomavirusin Kaposi sarcoma lesions

Presence ofBetapapillomavirusin Kaposi sarcoma lesions

Comparison of real-time PCR and culture isolation in colostrum-deprived pigs immunized and challenged with Haemophilus parasuis

Doença de Glässer: Uma revisão

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