Development of polymorphic microsatellite markers for Dalbergia nigra (Papilionoideae), an endangered tree from the Brazilian Atlantic Forest

Abstract

Dalbergia nigra is an endangered tree restricted to the Brazilian Atlantic Forest. Ten polymorphic microsatellite loci were isolated and characterized in 47 trees from two populations. The total number of alleles per locus ranged from three to 12 alleles. The levels of observed and expected heterozygosities ranged from 0.304 to 0.740 and from 0.278 to 0.872, respectively. Significant deviations from Hardy-Weinberg were detected for only three loci in each population. No pair of loci exhibited significant linkage disequilibrium. These microsatellites provide an efficient tool to investigate genetic structure in forest remnants with the purpose of conservation of this species.

Development of Genomic SSR for the Subtropical Hardwood Tree Dalbergia hupeana and Assessment of Their Transferability to Other Related Species

“…The Dalbergia species are good sources of precious timber; a few tree species have been identified as rosewood, and genetic research has been performed on them. The genetic diversity of endangered species and their phylogenetic relationships have been unveiled through studies using molecular markers and DNA sequences [6][7][8][9][10][11]. However, many species in the Dalbergia genus have not been investigated, especially subtropical species, which are good sources of hardwood and also face threats from climate change and human activity [32][33][34][35].…”

“…In China, 29 species of the genus Dalbergia were recorded in Flora of China; 14 of these species are endemic, and one has been introduced [5]. A few studies have focused on the genetic diversity of some Dalbergia species [6][7][8][9][10][11]; however, many species have not been studied and the limited availability of molecular markers may be one of the primary reasons for this.…”

Fine-scale spatial genetic structure of Dalbergia nigra (Fabaceae), a threatened and endemic tree of the Brazilian Atlantic Forest

“…Total genomic DNA was extracted from 2.5 mg of cambium following the protocol of Novaes et al (2009) . Seven polymorphic nuclear microsatellite loci ( Dnig1 , Dnig2 , Dnig4 , Dnig5 , Dnig6 , Dnig7 and Dnig10 ), which have been previously isolated and characterised by Ribeiro et al (2009b) , were used for genetic analyses. PCR amplifications were performed using the conditions established by Ribeiro et al (2009b) , which included a universal fluorescent-labelled M13 tail primer, and following the protocol described by Schuelke (2000) .…”

“…Seven polymorphic nuclear microsatellite loci ( Dnig1 , Dnig2 , Dnig4 , Dnig5 , Dnig6 , Dnig7 and Dnig10 ), which have been previously isolated and characterised by Ribeiro et al (2009b) , were used for genetic analyses. PCR amplifications were performed using the conditions established by Ribeiro et al (2009b) , which included a universal fluorescent-labelled M13 tail primer, and following the protocol described by Schuelke (2000) . Microsatellite fragments were analysed in a MegaBACE 1,000 automated sequencer (Amersham Biosciences) using 0.1% Tween 20 and ROX-500 Size Standard (GE Healthcare), and scored using the MegaBACE Fragment Profiler 1.2 software (Amersham Biosciences).…”

Genetic Structure and Diversity of Dalbergia nigra from Brazilian Atlantic Forest Fragments

“…Later, allozyme markers were used for the genetic diversity study of Brazilian rosewood in forest fragments in Minas Gerais [2]. After the development of a set of ten polymorphic microsatellite loci for Brazilian rosewood [21], a broader genetic analysis could be carried out. For example, a higher population spatial genetic structure was reported in the adults rather than in saplings from the secondary forest fragment [22].…”