Development of porcine adenovirus-3 as an expression vector.

Reddy, P. Seshidhar; Idamakanti, Neeraja; Hyun, Bang-Hun; Tikoo, Suresh K.; Babiuk, Lorne A.

doi:10.1099/0022-1317-80-3-563

Cited by 63 publications

(59 citation statements)

References 29 publications

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“…The PAV isolates found in this study were highly similar to the previously described PAV3 IAF strain (26). This is a strain derived from PAV3 strain 6618 and adapted to pig kidney cells and which has been isolated in several countries (10,14).…”

Section: Discussionsupporting

confidence: 79%

Detection of Bovine and Porcine Adenoviruses for Tracing the Source of Fecal Contamination

Motes

Clemente-Casares

Hundesa

et al. 2004

Appl Environ Microbiol

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In this study, a molecular procedure for the detection of adenoviruses of animal origin was developed to evaluate the level of excretion of these viruses by swine and cattle and to design a test to facilitate the tracing of specific sources of environmental viral contamination. Two sets of oligonucleotides were designed, one to detect porcine adenoviruses and the other to detect bovine and ovine adenoviruses. The specificity of the assays was assessed in 31 fecal samples and 12 sewage samples that were collected monthly during a 1-year period. The data also provided information on the environmental prevalence of animal adenoviruses. Porcine adenoviruses were detected in 17 of 24 (70%) pools of swine samples studied, with most isolates being closely related to serotype 3. Bovine adenoviruses were present in 6 of 8 (75%) pools studied, with strains belonging to the genera Mastadenovirus and Atadenovirus and being similar to bovine adenoviruses of types 2, 4, and 7. These sets of primers produced negative results in nested PCR assays when human adenovirus controls and urban-sewage samples were tested. Likewise, the sets of primers previously designed for detection of human adenovirus also produced negative results with animal adenoviruses. These results indicate the importance of further studies to evaluate the usefulness of these tests to trace the source of fecal contamination in water and food and for environmental studies.

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Section: Discussionsupporting

confidence: 79%

Detection of Bovine and Porcine Adenoviruses for Tracing the Source of Fecal Contamination

Motes

Clemente-Casares

Hundesa

et al. 2004

Appl Environ Microbiol

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“…An alternative strategy to circumvent preexisting anti-Ad immunity is the use of nonhuman Ad serotypes (5,13,14,26,33,38,40,43,44,48,49,51,(59)(60)(61). Nonhuman primatederived Ad vaccine vectors were developed to overcome preexisting immunity to common human Ad serotypes and to broaden the repertoire of Ad vaccines for booster immunizations.…”

Section: Discussionmentioning

confidence: 99%

Induction of Protective Immunity to Anthrax Lethal Toxin with a Nonhuman Primate Adenovirus-Based Vaccine in the Presence of Preexisting Anti-Human Adenovirus Immunity

Hashimoto¹,

Boyer²,

Hackett

et al. 2005

Infect Immun

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) that a single administration of a recombinant serotype 5 adenovirus (Ad) vector expressing anthrax protective antigen (PA) provides rapid protection against anthrax lethal toxin challenge. However, approximately 35 to 50% of humans have preexisting neutralizing antibodies against Ad5. This study assesses the hypothesis that a recombinant adenovirus vaccine based on the nonhuman primate-derived serotype AdC7, against which humans do not have immunity, expressing PA (AdC7PA) will protect against anthrax lethal toxin even in the presence of preexisting anti-Ad5 immunity. Naive and Ad5-immunized BALB/c mice received (intramuscularly) 10 8 to 10 11 particle units (PU) of AdC7PA, Ad5PA (a human serotype Ad5-based vector expressing a secreted form of PA), or AdNull (an Ad5 vector with no transgene). Robust anti-PA immunoglobulin G and neutralizing antibodies were detected by 2 to 4 weeks following administration of AdC7PA to naive or Ad5 preimmunized mice, whereas low anti-PA titers were detected in Ad5-preimmunized mice following administration of Ad5PA. To assess protection in vivo, naive or mice previously immunized against Ad5 were immunized with AdC7PA or Ad5PA and then challenged with a lethal intravenous dose of Bacillus anthracis lethal toxin. Whereas Ad5PA protected naive mice against challenge with B. anthracis lethal toxin, Ad5PA was ineffective in mice that were previously immunized against Ad5. In contrast, AdC7PA functioned effectively not only to protect naive mice but also to protect Ad5-preimmunized mice, with 100% survival after lethal toxin challenge. These data suggest the nonhuman-based vector AdC7PA is an effective vaccine for the development of protective immunity against B. anthracis and importantly functions as a "sero-switch" base for an adenovirus vaccine to function in the context of preexisting anti-Ad immunity.

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“…It is widely used for the propagation of HAV5 recombinants with E1 deleted (5,16). In addition to HAVs, a number of nonhuman adenoviruses, such as bovine adenovirus type 3 (BAV3) (37), canine adenovirus type 2 (28), porcine adenovirus type 3 (45), and ovine adenovirus (23), have been developed as vectors for producing recombinant vaccines and also for their potential use in gene therapy.…”

mentioning

confidence: 99%

Development and Characterization of Bovine × Human Hybrid Cell Lines That Efficiently Support the Replication of both Wild-Type Bovine and Human Adenoviruses and Those with E1 Deleted

Olphen

Mittal

2002

J Virol

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The 293 cell line that was generated by transforming human embryonic kidney cells with human adenovirus type 5 (HAV5) early region 1 (E1) sequences is an excellent host for generating and growing HAV5 recombinants with E1 deleted, but it does not support the replication of bovine adenovirus type 3 (BAV3). Madin-Darby bovine kidney (MDBK), an established bovine cell line, is an excellent host for growing and plaquing BAV3. For the purpose of combining the unique characteristics of these two cell lines (293 and MDBK), we generated a number of bovine ؋ human hybrid (BHH) cell lines. Comparison of three BHH hybrid clones-BHH3, BHH8, and BHH2C-with 293-Puro (puromycin-resistant 293 cells) and MDBK-Neo (G418-resistant MDBK cells) cell lines for total cellular DNA content, species-specific surface markers, isoenzyme analysis, and karyotyping indicate that they are hybrid in nature. BHH clones constitutively expressed the E1 proteins (E1A, E1B-21kDa, and E1B-55kDa) of HAV5 and efficiently supported the replication of both wild-type and replication-incompetent bovine or human adenoviruses. Transient gene expression experiments with a plasmid encoding the bacterial ␤-galactosidase gene demonstrated that BHH cell hybrids seem to have better transfection efficiencies than either of the parental cell lines. These cell lines will be useful for isolating and growing replication-competent human or bovine adenovirus recombinants with E1 deleted and for the study of cellular or viral factors important for viral replication. The development of somatic cell hybrids appears to be a simple way of combining some of the desirable characteristics present separately in two parental cell lines.The most widespread use of somatic cell hybrids is the formation of hybridomas for monoclonal antibody production. They are generated by the fusion of antibody-producing lymphocytes from a mouse or rat immunized with an antigen of interest with a myeloma cell line (1, 29). Production of interand intraspecific cell hybrids by means of polyethylene glycol (PEG)-induced fusion for extending the life of mammalian cells has been demonstrated (42). Somatic cell hybrids have also been used as tools for studying the chromosomal assignment of genes (12,26,27), mapping normal as well as tumorrelated genes (47), and localizing the site-specific integration of adeno-associated proviral DNA into human chromosome 19 (31, 49). Hybridization between bovine ϫ rodent, mouse, or hamster were exploited for the mapping of bovine genes (51), and bovine ϫ mouse hybrid cells were used for studying the replication of mengo virus (7,30,55).Adenoviral early region 1 (E1) genes are the first set of the genes that are expressed and are necessary for virus replication (22, 50). Adenovirus recombinants with E1 deleted are routinely produced and grown in a permissive cell line that constitutively expresses E1 proteins (17). The 293 cell line constitutively expresses E1 proteins and was derived from human embryonic kidney cells after transformation with human adenovirus type 5 (HA...

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Development of porcine adenovirus-3 as an expression vector.

Cited by 63 publications

References 29 publications

Detection of Bovine and Porcine Adenoviruses for Tracing the Source of Fecal Contamination

Detection of Bovine and Porcine Adenoviruses for Tracing the Source of Fecal Contamination

Induction of Protective Immunity to Anthrax Lethal Toxin with a Nonhuman Primate Adenovirus-Based Vaccine in the Presence of Preexisting Anti-Human Adenovirus Immunity

Development and Characterization of Bovine × Human Hybrid Cell Lines That Efficiently Support the Replication of both Wild-Type Bovine and Human Adenoviruses and Those with E1 Deleted

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