Development of Porcine Pancreatic Hydrolases and Their Isoenzymes from the Fetal Period to Adulthood

Weström, Björn; Ohlsson, Björn; Karlsson, Börje W.

doi:10.1097/00006676-198709000-00016

Cited by 20 publications

(17 citation statements)

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“…In addition, following birth, there is a major change in the nature of the enzymes present in pig pancreas with the gradual appearance of chymotrypsin C, cationic trypsin and protease E [103,131]. Accord [109].…”

Section: Effect Of Age On Digestive Productions and Enzyme Expressionsmentioning

confidence: 99%

Development of gastrointestinal and pancreatic functions in mammalians (mainly bovine and porcine species): influence of age and ingested food

Zabielski¹,

Huërou-Luron²,

Guilloteau³

1999

Reprod. Nutr. Dev.

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-This review summarizes recent advances in knowledge on the development of digestive tissues and their productions as well as mechanisms of regulation in response to age and ingested food in mammalian species (mainly bovine and porcine species). In the first two sections, changes are reported for stomach, pancreas and small intestine, and examined in relation to different situations (colostral, milk feeding and weaned periods). The implication of some regulatory substances (growth factors, gut regulatory peptides and neurohormonal substances) in regulation mechanisms is discussed over these periods. For

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Section: Effect Of Age On Digestive Productions and Enzyme Expressionsmentioning

confidence: 99%

Development of gastrointestinal and pancreatic functions in mammalians (mainly bovine and porcine species): influence of age and ingested food

Zabielski¹,

Huërou-Luron²,

Guilloteau³

1999

Reprod. Nutr. Dev.

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“…It is suggested that the histone H1 proteins mediate the binding to brush borders by ionic interactions, as previously modeled with small basic peptides and membranes containing acidic lipids (55). Because of the low levels of trypsin (56) and the presence of trypsin inhibitors in the intestines of neonatal piglets (57) and maternal colostrum (58), surfacebound histone H1 is not expected to be degraded by trypsin. Additional mechanisms appear to protect the histone H1 from intestinal proteases, since the 987P protein receptor was also observed on the brush borders of older pigs (29).…”

Section: Discussionmentioning

confidence: 88%

Histone H1 Proteins Act As Receptors for the 987P Fimbriae of Enterotoxigenic Escherichia coli

Zhu¹,

Chen

Choi³

et al. 2005

Journal of Biological Chemistry

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The tip adhesin FasG of the 987P fimbriae of enterotoxigenic Escherichia coli mediates two distinct adhesive interactions with brush border molecules of the intestinal epithelial cells of neonatal piglets. First, FasG attaches strongly to sulfatide with hydroxylated fatty acyl chains. This interaction involves lysine 117 and other lysine residues of FasG. Second, FasG recognizes specific intestinal brush border proteins that migrate on a sodium-dodecyl sulfate-polyacrylamide gel like a distinct set of 32-35-kDa proteins, as shown by ligand blotting assays. The protein sequence of high performance liquid chromatography-purified tryptic fragments of the major protein band matched sequences of human and murine histone H1 proteins. Porcine histone H1 proteins isolated from piglet intestinal epithelial cells demonstrated the same SDS-PAGE migration pattern and 987P binding properties as the 987P-specific protein receptors from porcine intestinal brush borders. Binding was dose-dependent and shown to be specific in adhesion inhibition and gel migration shift assays. Moreover, mapping of the histone H1 binding domain suggested that it is located in their lysine-rich C-terminal domains. Histone H1 molecules were visualized on the microvilli of intestinal epithelial cells by immunohistochemistry and electron microscopy. Taken together these results indicated that the intestinal protein receptors for 987P are histone H1 proteins. It is suggested that histones are released into the intestinal lumen by the high turnover of the intestinal epithelium. Their strong cationic properties can explain their association with the negatively charged brush border surfaces. There, the histone H1 molecules stabilize the sulfatide-fimbriae interaction by simultaneously binding to the membrane and to 987P. Enterotoxigenic Escherichia coli (ETEC)1 cause diarrhea in mammals by expressing at least one type of enteroadhesive fimbria and one type of enterotoxin. By adhering to intestinal epithelial cells, localized multiplication of an ETEC strain can progress to mucosal surface colonization and concomitant effective enterotoxin delivery. This was illustrated with hostspecific ETEC strains in both animals and human volunteers (1-5). ETEC are the most important etiologic agents of both neonatal and postweaning diarrhea in pigs (6, 7). 987P-fimbriated ETEC cause diarrhea in neonatal piglets in the United States (8), Europe (9, 10), Asia (11-15), and Central and South America (16 -18). In addition to this widespread distribution, the recent identification of human ETEC strains with 987P-like fimbriae (19, 20) on various continents highlights the evolutionary adaptability of these fimbriae (21).The 987P fimbria consists of the helical arrangement of protein subunits along a filamentous axis (22,23). It is a heteropolymeric structure that is made up of one major subunit, FasA, and two minor subunits, FasF and FasG (24). Fimbriae are not produced in the absence of any of these subunits (25). Electron microscopy and export studies indicated that FasG ...

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“…Both gastrin [1] and pancreatic enzyme activities [2] increase significantly in the second half of the fetal period in swine. Intestinal dipeptidase activi ty also undergoes marked changes during ges tation in fetal pigs, those changes being most significant during the embryonic period [3], Ontogenetic development of the digestive en zymes in the pig also occurs in the perinatal period [2][3][4][5][6]. The developmental changes oc curring during the first postnatal day are of special interest because of the digestive adap tation taking place in the gastrointestinal tract due to the change to extrauterine life.…”

Section: Introductionmentioning

confidence: 97%

Digestive Enzyme Development in Newborn Piglets Born of Sows Immunized against Somatostatin and/or Receiving Growth Hormone-Releasing Factor during Gestation

Farmer

Brazeau²,

Morisset³

1993

Neonatology

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Thirty-eight second parity sows were either immunized (IMM) against somatostatin (SRIF) and/or injected with growth hormone-releasing factor (GRF) during gestation. Treatment effects on pancreatic, gastric and duodenal development as well as on digestive enzyme activity of piglets at birth (before suckling) or at 24 h postpartum were investigated. Birth weights of piglets were similar across treatments (p > 0.1). Weight, DNA, RNA, total protein content and enzyme activity for all three organs increased between birth and 24 h postpartum (p < 0.01), except for pancreatic RNA and chymotrypsin which decreased (p < 0.01), and protein content of the pancreas which was unaltered (p > 0.1). Gastric RNA, pancreatic weight DNA, RNA:DNA and amylase:DNA ratios were increased in 1-day-old piglets from SRIF-IMM sows (p < 0.05). GRF only had significant effects (p < 0.05) on the maltase:DNA ratio, which it decreased. Yet, there were tendencies (p < 0.1) for duodenal weight, DNA and total protein content to be increased in piglets from GRF-injected dams. It is therefore apparent that major developmental changes of the pancreas, stomach and duodenum of piglets take place during the first 24 h postpartum. Injections of GRF and/or immunization against SRIF during gestation in swine also have several effects on digestive enzyme activity of neonatal pigs. Yet, the physiological implications of these early changes are not clear at the present time.

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Development of Porcine Pancreatic Hydrolases and Their Isoenzymes from the Fetal Period to Adulthood

Cited by 20 publications

References 0 publications

Development of gastrointestinal and pancreatic functions in mammalians (mainly bovine and porcine species): influence of age and ingested food

Development of gastrointestinal and pancreatic functions in mammalians (mainly bovine and porcine species): influence of age and ingested food

Histone H1 Proteins Act As Receptors for the 987P Fimbriae of Enterotoxigenic Escherichia coli

Digestive Enzyme Development in Newborn Piglets Born of Sows Immunized against Somatostatin and/or Receiving Growth Hormone-Releasing Factor during Gestation

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