Development of Pretreatment Method for Analysis of Vitamin
                        B12 in Cereal Infant Formula using Immunoaffinity Chromatography
                    and High-Performance Liquid Chromatography

Park, Jung Min; Koh, Jong Ho; Kim, Jin Man

doi:10.5851/kosfa.2020.e92

Cited by 2 publications

(1 citation statement)

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“…Due to the bacteria present in animals, the vitamin accumulates in animal products such as meat or milk. Plant foods are therefore largely free of vitamin B 12 [3]. The purely vegan diet is not sufficient to cover the daily requirement of vitamin B 12 and can therefore lead to a deficiency [2].…”

Section: Introductionmentioning

confidence: 99%

Determination of vitamin B₁₂ in commercial pepsin preparations using immunoaffinity chromatography and LC‐MS/MS

et al. 2023

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Vitamin B12 is a critical micronutrient in a vegan diet and thus dietary sources are limited and often low in the vitamin. Analytical determination of vitamin B12 requires laborious sample preparations including the proteolytic breakdown of the (food) matrix with proteases and the enrichment of the vitamin via immunoaffinity methods. Proteases like pepsin have an animal origin and thus might contain significant amounts of vitamin B12. We hypothesize that pepsin preparations potentially transfer residual vitamin B12 into the sample and falsify results. Hence the aim of our study was to determine trace amounts of cyano‐cobalamin by LC‐MS/MS in commercial protease enzyme preparations. The use of a stable 15N‐labeled cyano‐cobalamin as internal standard enabled us to detect vitamin B12 with a LOQ as low as 0.7 µg/l. Significant amounts of the vitamin were found in pepsin whereas the vitamin B12 content of papain preparations was below the detection limit. We conclude that sample preparation with pepsin bears the risk of false positive results, especially for samples with low vitamin B12 content, and we therefore recommend papain as an alternative protease for food sample preparation.

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Section: Introductionmentioning

confidence: 99%

Determination of vitamin B₁₂ in commercial pepsin preparations using immunoaffinity chromatography and LC‐MS/MS

et al. 2023

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Development of a simple and sensitive HPLC-DAD method for quantification of vitamin B12 fortified in infant food

Fan

Zhang

2021

Anal. Methods

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Development of Pretreatment Method for Analysis of Vitamin B12 in Cereal Infant Formula using Immunoaffinity Chromatography and High-Performance Liquid Chromatography

Cited by 2 publications

References 21 publications

Determination of vitamin B₁₂ in commercial pepsin preparations using immunoaffinity chromatography and LC‐MS/MS

Determination of vitamin B₁₂ in commercial pepsin preparations using immunoaffinity chromatography and LC‐MS/MS

Development of a simple and sensitive HPLC-DAD method for quantification of vitamin B12 fortified in infant food

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Development of Pretreatment Method for Analysis of Vitamin B12 in Cereal Infant Formula using Immunoaffinity Chromatography and High-Performance Liquid Chromatography

Cited by 2 publications

References 21 publications

Determination of vitamin B12 in commercial pepsin preparations using immunoaffinity chromatography and LC‐MS/MS

Determination of vitamin B12 in commercial pepsin preparations using immunoaffinity chromatography and LC‐MS/MS

Development of a simple and sensitive HPLC-DAD method for quantification of vitamin B12 fortified in infant food

Contact Info

Product

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Determination of vitamin B₁₂ in commercial pepsin preparations using immunoaffinity chromatography and LC‐MS/MS

Determination of vitamin B₁₂ in commercial pepsin preparations using immunoaffinity chromatography and LC‐MS/MS