Development of quasi-multicellular bodies of Treponema denticola

Wolf, Violetta; Lange, R; Wecke, Jörg

doi:10.1007/bf00249126

Cited by 18 publications

(1 citation statement)

References 13 publications

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“…The FlaA proteins are suggested to impart stiffness to the periplasmic flagella to give more swimming speed, whereas FlaB proteins may have effects on the morphology of the periplasmic flagella, maintaining the helicity of the filament, since a study reported that mutants of FlaB1 and 2 are completely nonhelical. 78 Altering the molecular stoichiometry of these subunits may therefore enable T. denticola to assume different morphologies, such as plaits and spherical bodies 79 or else modulate its speed or capacity to move in different environments.…”

Section: Locomotionmentioning

confidence: 99%

Differential Proteomic Analysis of a Polymicrobial Biofilm

et al. 2012

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Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia exist in a polymicrobial biofilm associated with chronic periodontitis. The aim of this study was to culture these three species as a polymicrobial biofilm and to determine proteins important for bacterial interactions. In a flow cell all three species attached and grew as a biofilm; however, after 90 h of culture P. gingivalis and T. denticola were closely associated and dominated the polymicrobial biofilm. For comparison, planktonic cultures of P. gingivalis and T. denticola were grown separately in continuous culture. Whole cell lysates were subjected to SDS-PAGE, followed by in-gel proteolytic H2 16O/H2 18O labeling. From two replicates, 135 and 174 P. gingivalis proteins and 134 and 194 T. denticola proteins were quantified by LC–MALDI TOF/TOF MS. The results suggest a change of strategy in iron acquisition by P. gingivalis due to large increases in the abundance of HusA and HusB in the polymicrobial biofilm while HmuY and other iron/haem transport systems decreased. Significant changes in the abundance of peptidases and enzymes involved in glutamate and glycine catabolism suggest syntrophy. These data indicate an intimate association between P. gingivalis and T. denticola in a biofilm that may play a role in disease pathogenesis.

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Section: Locomotionmentioning

confidence: 99%