Development of rapid gold nanoparticles based lateral flow assays for simultaneous detection of <i>Shigella</i> and <i>Salmonella</i> genera

Yahaya, Mohammad Lukman; Zakaria, Norhayati; Noordin, Rahmah; Razak, Khairunisak Abdul

doi:10.1002/bab.2029

Cited by 13 publications

(4 citation statements)

References 45 publications

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“…The sensitivity and specificity of the outcomes achieved from LFA have been similar to the ELISA test as the gold standard. Therefore, the multiplex LFA established in this study could be applied as a quick monitoring approach for the determination of diverse foodborne pathogens in food samples through a single test strip (M. L. Yahaya et al., 2020).…”

Section: Determination Of Pathogenic Bacteria Using Lfasmentioning

confidence: 99%

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

Sohrabi

Majidi

Khaki

et al. 2022

Comp Rev Food Sci Food Safe

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Diverse chemicals and some physical phenomena recently introduced in nanotechnology have enabled scientists to develop useful devices in the field of food sciences. Concerning such developments, detecting foodborne pathogenic bacteria is now an important issue. These kinds of bacteria species have demonstrated severe health effects after consuming foods and high mortality related to acute cases. The most leading path of intoxication and infection has been through food matrices. Hence, quick recognition of foodborne bacteria agents at low concentrations has been required in current diagnostics. Lateral flow assays (LFAs) are one of the urgent and prevalently applied quick recognition methods that have been settled for recognizing diverse types of analytes. Thus, the present review has stressed on latest developments in LFAs‐based platforms to detect various foodborne pathogenic bacteria such as Salmonella, Listeria, Escherichia coli, Brucella, Shigella, Staphylococcus aureus, Clostridium botulinum, and Vibrio cholera. Proper prominence has been given on exactly how the labels, detection elements, or procedures have affected recent developments in the evaluation of diverse bacteria using LFAs. Additionally, the modifications in assays specificity and sensitivity consistent with applied food processing techniques have been discussed. Finally, a conclusion has been drawn for highlighting the main challenges confronted through this method and offered a view and insight of thoughts for its further development in the future.

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Section: Determination Of Pathogenic Bacteria Using Lfasmentioning

confidence: 99%

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

Sohrabi

Majidi

Khaki

et al. 2022

Comp Rev Food Sci Food Safe

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“…Then, there is a nitrocellulose membrane with immobilized anti-analyte antibodies which react with this labeled flowing complex, revealing a line. A second control line made of anti-IgG antibodies is usually incorporated, which will react with the labeled antibodies either if they reacted with their corresponding targets or not [ 9 , 20 ]. A similar although slightly different approach was used by Shi et al in their multiplex sensor for neomycin and lincomycin in milk [ 10 ].…”

Section: Optical Sensorsmentioning

confidence: 99%

“…These types of sensors need specific antibodies, NPs, or other complexes to guarantee selectivity in the analysis. Milk is one of the tested matrices, with this type of platform and different assay formats: direct detection [ 82 , 83 ], displacement [ 19 ], or sandwich [ 20 ]. Although using direct detection, samples need to be incubated between 3 and 18 h depending on the bacteria to be analyzed.…”

Section: Other Sensing Platforms In Food Safetymentioning

confidence: 99%

Progress and Challenge of Sensors for Dairy Food Safety Monitoring

Fernández González,

Badía Laíño,

Costa-Fernández

et al. 2024

Sensors

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One of the most consumed foods is milk and milk products, and guaranteeing the suitability of these products is one of the major concerns in our society. This has led to the development of numerous sensors to enhance quality controls in the food chain. However, this is not a simple task, because it is necessary to establish the parameters to be analyzed and often, not only one compound is responsible for food contamination or degradation. To attempt to address this problem, a multiplex analysis together with a non-directed (e.g., general parameters such as pH) analysis are the most relevant alternatives to identifying the safety of dairy food. In recent years, the use of new technologies in the development of devices/platforms with optical or electrochemical signals has accelerated and intensified the pursuit of systems that provide a simple, rapid, cost-effective, and/or multiparametric response to the presence of contaminants, markers of various diseases, and/or indicators of safety levels. However, achieving the simultaneous determination of two or more analytes in situ, in a single measurement, and in real time, using only one working ‘real sensor’, remains one of the most daunting challenges, primarily due to the complexity of the sample matrix. To address these requirements, different approaches have been explored. The state of the art on food safety sensors will be summarized in this review including optical, electrochemical, and other sensor-based detection methods such as magnetoelastic or mass-based sensors.

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“… 27 In addition, lateral flow assays using labelled Au NCs and biosensors for their applications can also be applied to the detection of Shigella . 28 , 29 In the treatment of diseases, Au NCs can be used as drug carriers to specifically target and attack target cells. Au NCs have proven to be promising delivery vectors in the treatment of bacterial infections and tumours, 30 , 31 such as Staphylococcus aureus infections.…”

Section: Introductionmentioning

confidence: 99%

Au Nanoclusters Ameliorate Shigella Infectious Colitis by Inducing Oxidative Stress

Wu¹,

Chen²,

Zhang³

et al. 2021

IJN

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Background: Shigella infection has always been a global burden, and it particularly threatens children between the ages of 1 and 5 years. Economically underdeveloped countries are dominated by Shigella flexneri infection. The most effective method to treat Shigella is antibiotics, but with the abuse of antibiotics and the prevalence of multidrug resistance, we urgently need a relatively safe non-antibiotic treatment to replace it. Ultrasmall Au nanoclusters (NCs) have special physical and chemical properties and can better interact with and be internalized by bacteria to disrupt the metabolic balance. The purpose of this study was to explore whether Au NCs may be a substitute for antibiotics to treat Shigella infections. Methods: Au NCs and Shigella Sf301, R2448, and RII-1 were cocultured in vitro to evaluate the bactericidal ability of Au NCs. The degree of damage and mode of action of Au NCs in Shigella were clearly observed in images of scanning electron microscopy (SEM).In vivo experiments were conducted to observe the changes in body weight, clinical disease activity index (DAI) and colon (including length and histopathological sections) of mice treated with Au NCs. The effect of Au NCs was analysed by measuring the content of lipocalin-2 (LCN2) and Shigella in faeces. Next, the changes in Shigella biofilm activity, the release of reactive oxygen species (ROS), the changes in metabolism-related and membranerelated genes, and the effect of Au NCs on the body weight of mice were determined to further analyse the mechanism of action and effect. Results: Au NCs (100 μM) interfered with oxidative metabolism genes, induced a substantial increase in ROS levels, interacted with the cell membrane to destroy it, significantly killed Shigella, and effectively alleviated the intestinal damage caused by Shigella in mice. The activity of the biofilm formed by Shigella was reduced. Conclusion:The effective antibacterial effect and good safety suggest that Au NCs represent a good potential alternative to antibiotics to treat Shigella infections.

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Development of rapid gold nanoparticles based lateral flow assays for simultaneous detection of Shigella and Salmonella genera

Cited by 13 publications

References 45 publications

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

State of the art: Lateral flow assays toward the point‐of‐care foodborne pathogenic bacteria detection in food samples

Progress and Challenge of Sensors for Dairy Food Safety Monitoring

Au Nanoclusters Ameliorate Shigella Infectious Colitis by Inducing Oxidative Stress

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