Development of real-time radioisotope imaging systems for plant nutrient uptake studies

Kanno, Satoshi; Yamawaki, Masato; Ishibashi, H.; Kobayashi, Natsuko I.; Hirose, Atsushi; Tanoi, Keitaro; Nussaume, Laurent; Nakanishi, Teruyuki

doi:10.1098/rstb.2011.0229

Cited by 43 publications

(41 citation statements)

References 18 publications

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“…Consistent with PHT1 transporters being induced by Pi starvation (Misson et al, 2005;Kanno et al, 2012), a higher Pi influx capacity was observed in Pi-starved seedlings (compare -Pi with +Pi in Fig. 2B).…”

Section: Ptn Alters the Pi Transporter Regulations Triggered By Pi Stsupporting

confidence: 78%

Identification of Phosphatin, a Drug Alleviating Phosphate Starvation Responses in Arabidopsis

et al. 2014

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Inorganic phosphate (Pi) is present in most soils at suboptimal concentrations, strongly limiting plant development. Plants have the ability to sense and adapt to the surrounding ionic environment, and several genes involved in the response to Pi starvation have been identified. However, a global understanding of the regulatory mechanisms involved in this process is still elusive. Here, we have initiated a chemical genetics approach and isolated compounds that inhibit the response to Pi starvation in Arabidopsis (Arabidopsis thaliana). Molecules were screened for their ability to inhibit the expression of a Pi starvation marker gene (the high-affinity Pi transporter PHT1;4). A drug family named Phosphatin (PTN; Pi starvation inhibitor), whose members act as partial suppressors of Pi starvation responses, was thus identified. PTN addition also reduced various traits of Pi starvation, such as phospholipid/glycolipid conversion, and the accumulation of starch and anthocyanins. A transcriptomic assay revealed a broad impact of PTN on the expression of many genes regulated by low Pi availability. Despite the reduced amount of Pi transporters and resulting reduced Pi uptake capacity, no reduction of Pi content was observed. In addition, PTN improved plant growth; this reveals that the developmental restrictions induced by Pi starvation are not a consequence of metabolic limitation but a result of genetic regulation. This highlights the existence of signal transduction pathway(s) that limit plant development under the Pi starvation condition.

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Section: Ptn Alters the Pi Transporter Regulations Triggered By Pi Stsupporting

confidence: 78%

Identification of Phosphatin, a Drug Alleviating Phosphate Starvation Responses in Arabidopsis

et al. 2014

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“…These cluster roots are formed as part of the normal root developmental programme rather than as a responsive change [105], and are an example of a root structure which appears to have evolved primarily for the maximization of phosphorus acquisition from the soil [106,107]. In this issue, Nussaume et al [108] discuss a novel imaging technique for the visualization of nutrient uptake.…”

Section: Control Of Root Branching In Arabidopsis (A) Hormonesmentioning

confidence: 99%

Root system architecture: insights fromArabidopsisand cereal crops

Smith

Smet

2012

Phil. Trans. R. Soc. B

383

289

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Roots are important to plants for a wide variety of processes, including nutrient and water uptake, anchoring and mechanical support, storage functions, and as the major interface between the plant and various biotic and abiotic factors in the soil environment. Understanding the development and architecture of roots holds potential for the exploitation and manipulation of root characteristics to both increase food plant yield and optimize agricultural land use. This theme issue highlights the importance of investigating specific aspects of root architecture in both the model plant Arabidopsis thaliana and (cereal) crops, presents novel insights into elements that are currently hardly addressed and provides new tools and technologies to study various aspects of root system architecture. This introduction gives a broad overview of the importance of the root system and provides a snapshot of the molecular control mechanisms associated with root branching and responses to the environment in A. thaliana and cereal crops.

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“…HPLC (Uwimana et al 2012). A more sophisticated technique was the imaging of ionic movements in plants using real-time radioisotope imaging systems (in this case 32 P) by Kanno et al 2012, which however appears to be too complex for efficient screening. Recently, a system using NMR or positron emission tomography provided very useful information, but would be even more difficult to apply in practice (De Smet et al 2012).…”

Section: Hydroponics: Growing Plants On Water Solutionsmentioning

confidence: 99%

Improving phosphorus use efficiency in agriculture: opportunities for breeding

Wiel¹,

Linden²,

Scholten³

2015

Euphytica

195

115

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Phosphorus (P) is often an important limiting factor for crop yields, but rock phosphate as fertilizer is a non-renewable resource and expected to become scarce in the future. High P input levels in agriculture have led to environmental problems. One of the ways to tackle these issues simultaneously is improving phosphorus use efficiency (PUE) of the crops through breeding. In this review, we describe plant architectural and physiological traits important for PUE. Subsequently, we discuss efficient methods of screening for PUE traits. We address targeted cultivation methods, including solid and hydroponic systems, as well as testing methods, such as image analysis systems, and biomass and photosynthesis measurements. Genetic variation for PUE traits has been assessed in many crops, and genetics of PUE has been studied by quantitative trait loci (QTL) analyses and genome-wide association study. A number of genes involved in the plant's response to low P have been characterized. These genes include transcription factors, and genes involved in signal transduction, hormonal pathways, sugar signalling, P saving metabolic pathways, and in P scavenging, including transporters and metabolites and/or ATP-ases mobilizing P in the soil. In addition, the role of microorganisms promoting PUE of plants, particularly arbuscular mycorrhizal fungi is discussed. An overview is given of methods for selecting for optimal combinations of plant and fungal genotypes, and their genetics, incl. QTLs and genes involved. In conclusion, significant progress has been made in selecting for traits for PUE, developing systems for the difficult but highly relevant root phenotyping, and in identifying QTLs and genes involved.

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Development of real-time radioisotope imaging systems for plant nutrient uptake studies

Cited by 43 publications

References 18 publications

Identification of Phosphatin, a Drug Alleviating Phosphate Starvation Responses in Arabidopsis

Identification of Phosphatin, a Drug Alleviating Phosphate Starvation Responses in Arabidopsis

Root system architecture: insights fromArabidopsisand cereal crops

Improving phosphorus use efficiency in agriculture: opportunities for breeding

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