Development of recombinant chicken IgY from single chain fragment of variable region for diagnosis of BSE

Miyamoto, Katsunari; Shimamoto, Tadashi; Aosasa, Masayoshi; Kimura, Sota; Nakamura, Naoto; Okubo, Y.; Yokoyama, Takashi; Horiuchi, Hiroyuki; Furusawa, Shuichi; Matsuda, Haruo

doi:10.1016/j.biologicals.2006.01.003

Cited by 15 publications

(6 citation statements)

References 18 publications

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“…In another study, a sdAb produced in camels immunized with intracellular bacteria Brucella melitensis , which is responsible for Mediterranean fever in animals and humans, could recognize antigens of a B. melitensis in ELISA ( 21 ). Finally, recombinant chicken immunoglobulin Y (IgY) (Ab3-15 and Ab4-19) anti-prion protein (PrP sc ) was produced to diagnose BSE, showing that it can be used to diagnose BSE and other prion diseases ( 22 ).…”

Section: Recombinant Antibodies In Veterinary Diagnosismentioning

confidence: 99%

Recombinant Antibodies in Veterinary Medicine: An Update

2018

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The production of recombinant antibodies has had a tremendous impact on several research fields, most prominently in biotechnology, immunology and medicine, enabling enormous advances in each. Thus far, a broad diversity of recombinant antibody (rAb) forms have been designed and expressed using different expression systems. Even though the majority of rAbs approved for clinical use are targeted to humans, advances in veterinary medicine seem promising. The aim of this mini-review is to present an update regarding the rAbs in veterinary medicine reported to date, as well as their potential use in diagnostics, prophylaxis and therapeutics. Full- and single-chain fragment variables are the most common forms of rAbs developed for the detection, prevention and control of parasitic, bacterial and viral diseases, as well as pain and cancer treatment. Nonetheless, advances in research seem to be skewed toward economically important animals, such as pigs, cows, poultry and dogs. Although significant results have been obtained from the rAbs reported here, most have not been developed enough to be approved. Further research and clinical trials should be encouraged to enable important findings to fulfill their intended potential to improve animal well-being.

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Section: Recombinant Antibodies In Veterinary Diagnosismentioning

confidence: 99%

Recombinant Antibodies in Veterinary Medicine: An Update

2018

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“…Single-chain variable fragments (scFvs) based on chicken immunoglobulins are particularly suitable as a basis for developing immunotests, not only because they are serologically distinct from mammalian immunoglobulins, but because the avian antibody repertoire can be accessed [9][10][11] more readily than that of any mammal other than the camelids [12]. Recombinant chicken antibodies derived either from large ''universal'' or dedicated immune repertoires have been shown to be eminently useful in a variety of immunotests aimed at detecting either antigens or antibodies [13][14][15][16][17][18][19]. This suggests that if judiciously applied, they could also play a role in the diagnosis of tuberculosis.…”

Section: Introductionmentioning

confidence: 99%

Chicken scFvs and bivalent scFv-CH fusions directed against HSP65 of Mycobacterium bovis

et al. 2010

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a b s t r a c tTwo chicken single-chain variable region antibody fragments (scFvs) that recognised the 65 kDa heatshock protein (HSP65) of Mycobacterium bovis were selected from a large semi-synthetic phage displayed library. Both recognised HSP65 in indirect enzyme-linked immunosorbent assay (ELISA) and immunoblots and retained their activity during storage. Neither, however, could function as the capture reagent in a sandwich ELISA when immobilised on polystyrene. To establish whether they could be engineered for general use in immunotests, the genes coding for these scFvs were subcloned in expression vectors that contained sequences encoding chicken IgY heavy-chain constant region domains. This resulted in larger bivalent constructs which more closely resembled IgY molecules. The engineered fragments were evaluated in ELISAs and gold-conjugated immunochromatographic tests (ICTs). In contrast to their previous behaviour as scFvs, the modified fragments (designated ''gallibodies'') could be used for immunocapture in ELISA and could be readily conjugated to colloidal gold nanoparticles. A sandwich ICT that could detect recombinant HSP65 was also devised. Although converting the recombinant single-chain monomeric antibody fragments to bivalent immunoglobulin-like molecules did not entirely 'standardise' the behaviour of the scFvs, this approach remains potentially useful for developing practical, robust, immunodiagnostic reagents.

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“…IgY polyclonal antibodies are also an attractive source for development of sensitive immunoassays compared to mammalian antibodies (Cova, 2005; Miyamoto et al, 2007a,b; Sunwoo et al, 2006). The generation of BsMAbs as bifunctional immunoconjugates bearing two different binding sites (paratopes) for SARS-CoV NP avoids the need for random chemical coupling strategies.…”

Section: Introductionmentioning

confidence: 99%

A rapid point of care immunoswab assay for SARS-CoV detection

Kammila

Das

Bhatnagar

et al. 2008

Journal of Virological Methods

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The emergence of severe acute respiratory syndrome (SARS) resulted in several outbreaks worldwide. Early tests for diagnosis were not always conclusive in identifying a SARS suspected patient. Nucleocapsid protein (NP) is the most predominant virus derived structural protein which is shed in high amounts in serum and nasopharyngeal aspirate during the first week of infection. As part of such efforts, a simple, easy to use immunoswab method was developed by generating a panel of monoclonal antibodies (MAbs), Bispecific MAbs and chicken polyclonal IgY antibody against the SARS-CoV nucleocapsid protein (NP). Employing the MAb-based immunoswab, an NP concentration of 200 pg/mL in saline and pig nasopharyngeal aspirate, and 500 pg/mL in rabbit serum were detected. BsMAb-based immunoswabs detected an NP concentration of 20 pg/mL in saline, 500 pg/mL in rabbit serum and 20-200 pg/mL in pig nasopharyngeal aspirate. Polyclonal IgY-based immunoswabs detected an NP concentration of 10 pg/mL in pig nasopharyngeal aspirate providing the most sensitive SARS point of care assay. Results show that the robust immunoswab method of detecting SARS-CoV NP antigen can be developed into an easy and effective way of identifying SARS suspected individuals during a future SARS epidemic, thereby reducing and containing the transmission. The key feature of this simple immunoswab diagnostic assay is its ability to detect the presence of the SARS-CoV antigen within 45-60 min with the availability of the body fluid samples.

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Development of recombinant chicken IgY from single chain fragment of variable region for diagnosis of BSE

Cited by 15 publications

References 18 publications

Recombinant Antibodies in Veterinary Medicine: An Update

Recombinant Antibodies in Veterinary Medicine: An Update

Chicken scFvs and bivalent scFv-CH fusions directed against HSP65 of Mycobacterium bovis

A rapid point of care immunoswab assay for SARS-CoV detection

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