Development of recombinant Streptomyces for biotechnological and environmental uses

Crawford, Don L.

doi:10.1016/0734-9750(88)90004-3

Cited by 9 publications

(4 citation statements)

References 80 publications

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“…An alternative, protein-excreting procaryotic host which is recently gaining attention is Streptornyces Offprint requests to: G. F. Payne (Crawford 1988). This procaryote is commonly used in the pharmaceutical industry, and appropriate vectors are being developed to permit genetic modifications.…”

Section: Introductionmentioning

confidence: 99%

Improved production of heterologous protein from Streptomyces lividans

Payne

Delacruz

Coppella

1990

Appl Microbiol Biotechnol

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Protein-secreting procaryotic host organisms are currently being sought as alternatives to Escherichia coli for recombinant processing. In this study we examined how manipulation of the cultivation conditions can enhance heterologous protein production by Streptomyces lividans. The recombinant S. lividans used in this study expressed and excreted a Flavobacterium enzyme capable of hydrolyzing organophosphates. Initial shake-flask studies demonstrated that supplementing Luria-Bertani medium with moderate amounts of glucose (30 g/l), led to improved enzyme production. In fermentor studies with controlled pH, a further twofold increase in production was observed when glucose was fed continuously as compared to batch cultivation. This improved production in the glucose-fed culture may be related to a reduced accumulation of acids. Continuous feeding of both glucose and tryptone led to a further sixfold increase in production. In addition to enhancing production 25-fold, the efficiency of enzyme production and the specific activity of the excreted enzyme were also improved by glucose and tryptone feeding. These results demonstrate that in addition to genetic manipulations, optimization of cultivation conditions can lead to significant improvements in the production of heterologous proteins from Streptomyces.

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Section: Introductionmentioning

confidence: 99%

Improved production of heterologous protein from Streptomyces lividans

Payne

Delacruz

Coppella

1990

Appl Microbiol Biotechnol

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“…On the other hand, modifying water potential of the soil microcosms did not result in affecting the establishment of the Streptomyces liuiduns cells or the transfer rate. cent advances in genetic engineering in order to improve their producing capacities [4,5]. A wellestablished growth technology, the presence of numerous plasmids which are now widely used as cloning vectors [6] and the successful expression of foreign genes in several Streptomyces isolates contribute in supporting the industrial interest in such microorganisms and the increasing efforts in genetically engineering their properties [7-91.However, the wide-spread occurrence of a highlevel of genetic instability among Streptomyces requires particular attention to be paid to the cloning…”

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confidence: 99%

Efficiency of the transfer of a pSAM2-derivative plasmid between two strains of Streptomyces lividans in conditions ranging from agar slants to non-sterile soil microcosms

Clerc

1996

FEMS Microbiology Ecology

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The aim of this work was to determine the efficiency of the conjugative plasmid pTSl30 to transfer in various environmental conditions between two strains of Streptom_vces lividuns. This plasmid is a derivative of the conjugative and integrative plasmid pSAM2 isolated originally from Streptomyces ambofuciens and capable of transfer to a large range of bacteria. Our results demonstrate the high frequency of the conjugation mechanism since more than 60% of the recipient cells developed on agar slants harbored the plasmid pTS 130 (as evidenced by Southern hybridization with a pSAM2 derivative plasmid probe). When donor and recipient strains were inoculated into sterile and non-sterile soil microcosms, transconjugants were detected after two days of incubation in both cases. However, the number of donor, recipient and transconjugant cells were established at a lower level in the non-sterile soil than in the sterile soil experiments. Moreover, nutrient amendment of the sterile soil was found to increase the population levels of parental strains and transfer frequencies both significantly and simultaneously. On the other hand, modifying water potential of the soil microcosms did not result in affecting the establishment of the Streptomyces liuiduns cells or the transfer rate. cent advances in genetic engineering in order to improve their producing capacities [4,5]. A wellestablished growth technology, the presence of numerous plasmids which are now widely used as cloning vectors [6] and the successful expression of foreign genes in several Streptomyces isolates contribute in supporting the industrial interest in such microorganisms and the increasing efforts in genetically engineering their properties [7-91.However, the wide-spread occurrence of a highlevel of genetic instability among Streptomyces requires particular attention to be paid to the cloning

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“…Since the 1970s, recombinant DNA technology has revolutionized the ability to engineer microorganisms by modifying specific genes and pathways for optimized production of commercially significant metabolites [78]. In contrast, the concept of reverse engineering has evolved as a powerful tool in which two processes are utilized to genetically characterize existing overproducing strains, and a second generation of information is used for more efficient engineering of new strains that synthesize high yields of natural products [79].…”

Section: Engineered Proteomicsmentioning

confidence: 99%

An Insight into the “-Omics” Based Engineering of Streptomycetes for Secondary Metabolite Overproduction

Chaudhary

Dhakal

Sohng

2013

BioMed Research International

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Microorganisms produce a range of chemical substances representing a vast diversity of fascinating molecular architectures not available in any other system. Among them, Streptomyces are frequently used to produce useful enzymes and a wide variety of secondary metabolites with potential biological activities. Streptomyces are preferred over other microorganisms for producing more than half of the clinically useful naturally originating pharmaceuticals. However, these compounds are usually produced in very low amounts (or not at all) under typical laboratory conditions. Despite the superiority of Streptomyces, they still lack well documented genetic information and a large number of in-depth molecular biological tools for strain improvement. Previous attempts to produce high yielding strains required selection of the genetic material through classical mutagenesis for commercial production of secondary metabolites, optimizing culture conditions, and random selection. However, a profound effect on the strategy for strain development has occurred with the recent advancement of whole-genome sequencing, systems biology, and genetic engineering. In this review, we demonstrate a few of the major issues related to the potential of “-omics” technology (genomics, transcriptomics, proteomics, and metabolomics) for improving streptomycetes as an intelligent chemical factory for enhancing the production of useful bioactive compounds.

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Development of recombinant Streptomyces for biotechnological and environmental uses

Cited by 9 publications

References 80 publications

Improved production of heterologous protein from Streptomyces lividans

Improved production of heterologous protein from Streptomyces lividans

Efficiency of the transfer of a pSAM2-derivative plasmid between two strains of Streptomyces lividans in conditions ranging from agar slants to non-sterile soil microcosms

An Insight into the “-Omics” Based Engineering of Streptomycetes for Secondary Metabolite Overproduction

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