Development of Red Pigmentation in Chlorococcum wimmeri (Chlorophyta: Chlorococcales)

Brown, Thomas E.; Richardson, Frances L.; Vaughn, Mattie L.

doi:10.2216/i0031-8884-6-4-167.1

Cited by 46 publications

(19 citation statements)

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“…Chlamydomonas moewusii Gerloff (Chlamydomonadaceae) was obtained from the Culture Collection of Algae and Protozoa of Dunstaffnage Marine Laboratory (Scotland, UK) (strain CCAP 11/5B) and was maintained in sterile Bristol medium (Brown et al 1967).…”

Section: Microalgal Culturesmentioning

confidence: 99%

Flow cytometric analysis to evaluate physiological alterations in herbicide-exposed Chlamydomonas moewusii cells

et al. 2011

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Investigation of herbicide toxicology in non-target aquatic primary producers such as microalgae is of great importance from an ecological point of view. In order to study the toxicity of the widely used herbicide paraquat on freshwater green microalga Chlamydomonas moewusii, physiological changes associated with 96 h-exposures to this pollutant were monitored using flow cytometry (FCM) technique. Intracellular reactive oxygen species concentration, cytoplasmic membrane potential, metabolic activity and cell protein content were monitored to evaluate the toxicological impact of paraquat on algal physiology. Results showed that herbicide paraquat induced oxidative stress in C. moewusii cells, as it indicated the increase of both superoxide anion and hydrogen peroxide levels observed in non-chlorotic cells of cultures exposed to increasing herbicide concentrations. Furthermore, a progressive increase in the percentage of depolarised cells and a decrease in the metabolic activity level were observed in response to paraquat when non-chlorotic cells were analysed. Chlorotic cells were probably non-viable cells, based on the cytoplasmic membrane depolarisation, its metabolically non-active state and its drastically reduced protein content. In view of the obtained results, we have concluded that a range of significant physiological alterations, detected by flow cytometry, occur when C. moewusii, an ubiquitous microalga in freshwater environments, is challenged with environmentally relevant concentrations of the herbicide paraquat.

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Section: Microalgal Culturesmentioning

confidence: 99%

Flow cytometric analysis to evaluate physiological alterations in herbicide-exposed Chlamydomonas moewusii cells

et al. 2011

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“…This strain was obtained from the CCAP Culture Collection of Algae and Protozoa of Freshwater Ecology Institute (Cumbria, UK) (strain CCAP 11/5B). It was grown and maintained in autoclaved (1218C, 20 min) Bristol medium [17]. Microalgal cultures were incubated at a temperature of 18 AE 18C, illuminated with 68.25 mmol photon/(m 2 s), with a dark:light cycle of 12:12 h and continuously aerated with a constant flux of 10 L/min.…”

Section: Test Organism and Culture Conditionmentioning

confidence: 99%

Cadmium toxicity on the freshwater microalga Chlamydomonas moewusii Gerloff: Biosynthesis of thiol compounds

Suárez

Torres

Pérez-Rama

et al. 2010

Environmental Toxicology and Chemistry

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Cadmium (Cd) toxicity and production of different thiols (phytochelatins, glutathione, gamma-Glu-Cys and cysteine) were studied in the microalga Chlamydomonas moewusii exposed to different concentrations of this metal (1, 2, 4, 6, 8, and 10 mg/L) for 96 h. The inhibitory effect of Cd on growth was demonstrated. The value of EC50 (metal concentration which reduces the population growth to 50% of the control) obtained for this microalga was estimated at 4.1 +/- 0.8 mg/L of Cd after 96 h of exposure. The amount of thiol compounds synthesized by C. moewusii changed with Cd concentration. Cysteine concentrations were significantly higher compared to those of gamma-Glu-Cys and glutathione in all the Cd concentrations assayed. The amino acid cysteine reached its higher levels in those cultures in which a decrease in the concentration of phytochelatins (PCs) was observed. Both cysteine and glutathione concentrations showed significant differences along the Cd concentrations assayed, while the amount of gamma-Glu-Cys detected remained stable. The PCs detected were of two, three, and four subunits. The level of PC(2) was higher than that of PC(3) and PC(4). PC(4) was detected only in the cultures exposed to the Cd concentrations of 1 and 2 mg/L, in which the synthesis of phytochelatins was higher. A rapid increase in the production of PC(2) and PC(3) was observed up to a Cd concentration of 2 mg/L, after which their levels began to decrease. Phytochelatins were not detected in cultures without Cd (controls) and in those exposed to the maximum Cd concentration (10 mg/L), in which cell growth was completely inhibited.

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“…1D). Based on the existence and structure of these intermediates products and the previous reports on the biosynthesis of astaxanthin [10,27,29], possible pathways starting from bcarotene to astaxanthin with the introduction of a keto function group at C-4 or the introduction of a hydroxyl function group at C-3 and followed by random addition of keto or hydroxyl groups at both sides of the b-end groups were described in Fig. 2.…”

Section: Accumulation Of Secondary Carotenoidsmentioning

confidence: 99%

“…Although H. pluvialis possesses high content of astaxanthin, alternative astaxanthin-producing microalgae with the properties of fast growth rate are still important [9]. Chlorococcum wimerri [10], Botryococcus braunii [11], Chlorella zofingiensis [12] and Chlorococcum sp. [13] have been found to have the ability of astaxanthin accumulation.…”

Section: Introductionmentioning

confidence: 99%

The accumulation and metabolism of astaxanthin in Scenedesmus obliquus (Chlorophyceae)

Qin

Liu

2008

Process Biochemistry

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Development of Red Pigmentation in Chlorococcum wimmeri (Chlorophyta: Chlorococcales)

Cited by 46 publications

References 0 publications

Flow cytometric analysis to evaluate physiological alterations in herbicide-exposed Chlamydomonas moewusii cells

Flow cytometric analysis to evaluate physiological alterations in herbicide-exposed Chlamydomonas moewusii cells

Cadmium toxicity on the freshwater microalga Chlamydomonas moewusii Gerloff: Biosynthesis of thiol compounds

The accumulation and metabolism of astaxanthin in Scenedesmus obliquus (Chlorophyceae)

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