Development of reverse transcriptase polymerase chain reaction enzyme-linked immunosorbent assay for the detection of hepatitis A virus in vegetables

Tahk, Hongmin; Lee, Kang Bum; Lee, Min Hwa; Seo, Dong Joo; Cheon, Doo‐Sung; Choi, Changsun

doi:10.1016/j.foodcont.2011.07.012

Cited by 13 publications

(4 citation statements)

References 28 publications

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“…The results for determining the sensitivity well demonstrates the extraordinary capability of in situ magnetic beads based RT-PCR with ECL assay in detecting VP6 gene. And the sensitivity is higher than those nucleic acid-based viral pathogen detection platforms reported previously [25][26][27] . We have demonstrated in situ magnetic beads based RT-PCR with ECL detection for detection of rotavirus.…”

Section: The Sensitivity Of Current Assaymentioning

confidence: 65%

Development of an in situ magnetic beads based RT-PCR method for electrochemiluminescent detection of rotavirus

Zhan

Zhou

2012

SPIE Proceedings

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Rotaviruses are double-stranded RNA viruses belonging to the family of enteric pathogens. It is a major cause of diarrhoeal disease in infants and young children worldwide. Consequently, rapid and accurate detection of rotaviruses is of great importance in controlling and preventing food-and waterborne diseases and outbreaks. Reverse transcription-polymerase chain reaction (RT-PCR) is a reliable method that possesses high specificity and sensitivity. It has been widely used to detection of viruses. Electrochemiluminescence (ECL) can be considered as an important and powerful tool in analytical and clinical application with high sensitivity, excellent specificity, and low cost. Here we have developed a method for the detection of rotavirus by combining in situ magnetic beads (MBs) based RT-PCR with ECL.RT of rotavirus RNA was carried out in a traditional way and the resulting cDNA was directly amplified on MBs.Forward primers were covalently bounded to MBs and reverse primers were labeled with tris-(2, 2'-bipyridyl) ruthenium (TBR). During the PCR cycling, the TBR labeled products were directly loaded and enriched on the surface of MBs.Then the MBs-TBR complexes could be analyzed by a magnetic ECL platform without any post-modification or post-incubation，which avoid some laborious manual operations and achieve rapid yet sensitive detection. In this study, rotavirus from fecal specimens was successfully detected within 2 h, and the limit of detection was estimated to be 10 4 copies/μL. This novel in situ MBs based RT-PCR with ECL detection method can be used for pathogen detection in food safety field and clinical diagnosis.

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Section: The Sensitivity Of Current Assaymentioning

confidence: 65%

Development of an in situ magnetic beads based RT-PCR method for electrochemiluminescent detection of rotavirus

Zhan

Zhou

2012

SPIE Proceedings

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“…Samples were processed based on methods described by Tahk et al [28]. Strawberries and lettuce were cut to produce 5 g aliquots, which were washed with approximately 50 ml 0.25 M threonine-0.3 M NaCl on a rotary shaker for 5 h. The elution was then concentrated to <500 μl using Vivaspin-20 filters featuring a 100,000 nominal molecular weight cutoff value (Sartorius Stedim North America, Bohemia, NY).…”

Section: Methodsmentioning

confidence: 99%

Absolute quantification of norovirus capsid protein in food, water, and soil using synthetic peptides with electrospray and MALDI mass spectrometry

Hartmann

Colquhoun

Schwab

et al. 2015

Journal of Hazardous Materials

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Norovirus infections are one of the most prominent public health problems of microbial origin in the U.S. and other industrialized countries. Surveillance is necessary to prevent secondary infection, confirm successful cleanup after outbreaks, and track the causative agent. Quantitative mass spectrometry, based on absolute quantitation with stable-isotope labeled peptides, is a promising tool for norovirus monitoring because of its speed, sensitivity, and robustness in the face of environmental inhibitors. In the current study, we present two new methods for the detection of the norovirus genogroup I capsid protein using electrospray and matrixassisted laser desorption/ionization (MALDI) mass spectrometry. The peptide TLDPIEVPLEDVR was used to quantify norovirus-like particles down to 500 attomoles with electrospray and 100 attomoles with MALDI. With MALDI, we also demonstrate a detection limit of 1 femtomole and a quantitative dynamic range of 5 orders of magnitude in the presence of an environmental matrix effect. Due to the rapid processing time and applicability to a wide range of environmental sample types (bacterial lysate, produce, milk, soil, and groundwater), mass spectrometry-based absolute quantitation has a strong potential for use in public health and environmental sciences.

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“…Moreover, Tahk et al compared the three methods of polyethylene glycol precipitation, ultrafiltration membrane, and immunomagnetic separation for the concentration of HAV virus eluate in cabbage, lettuce, and sesame leaves [ 52 ]. For immunomagnetic separation, the surface of magnetic beads was first functionalized and modified with the antibody.…”

Section: Sample Pretreatment In Different Detection Platforms For mentioning

confidence: 99%

Food Safety in Post-COVID-19 Pandemic: Challenges and Countermeasures

Zhang

Zhu

et al. 2021

Biosensors

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Understanding food safety hazard risks is essential to avoid potential negative heath impacts in the food supply chain in a post-COVID-19 pandemic era. Development of strategies for virus direction in foods plays an important role in food safety and verification. Early warning, tracing, and detection should be implemented as an integrated system in order to mitigate thecoronavirus disease 2019 (COVID-19) outbreak, in which the detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is critical as it not only concerns screening of populations but also monitoring of possible contaminated sources such as the food supply chain. In this review, we point out the consequences in different aspects of our daily life in the post-COVID-19 pandemic from the perspective of the food supply chain and the food industry. We summarize the possible transmission routes of COVID-19 in the food supply chain before exploring the development of corresponding detection tools of SARS-CoV-2. Accordingly, we compare different detection methods for the virus in foods, including different pretreatments of food matrices in the virus detection. Finally, the future perspectives are proposed.

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Development of reverse transcriptase polymerase chain reaction enzyme-linked immunosorbent assay for the detection of hepatitis A virus in vegetables

Cited by 13 publications

References 28 publications

Development of an in situ magnetic beads based RT-PCR method for electrochemiluminescent detection of rotavirus

Development of an in situ magnetic beads based RT-PCR method for electrochemiluminescent detection of rotavirus

Absolute quantification of norovirus capsid protein in food, water, and soil using synthetic peptides with electrospray and MALDI mass spectrometry

Food Safety in Post-COVID-19 Pandemic: Challenges and Countermeasures

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