Development of reverse transcription recombinase polymerase amplification assay for avian influenza H5N1 HA gene detection

Yehia, Nahed; Arafa, Abdel-Satar; Wahed, Ahmed Abd El; El-Sanousi, Ahmed A.; Weidmann, Manfred; Shalaby, Mohamed A.

doi:10.1016/j.jviromet.2015.07.011

Cited by 38 publications

(28 citation statements)

References 31 publications

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“…In the developed PCV2 real-time RPA assay, a real-time PCR machine is available in our laboratory. A simple ESEQuant TS2 device (Qiagen, Germany) is also available in PCV2 real-time RPA assay [14, 17]. This device is simpler and cheaper than the real-time PCR machine, and it could be used conveniently in field as it could be charged by battery.…”

Section: Discussionmentioning

confidence: 99%

“…RPA has been developed for veterinary importance viruses detection, including porcine parvovirus virus [13], avian influenza virus [14], bovine viral diarrhea virus [15], canine parvovirus type 2 [16], foot-and-mouth disease virus [17], and Orf virus [18, 19]. In this study, we developed fluorescent probe-based real-time RPA assay (PCV2 real-time RPA assay) and RPA assay united with LFD (PCV2 RPA LFD assay) to detect PCV2.…”

Section: Introductionmentioning

confidence: 99%

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Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2

Yang

Qin

Sun

et al. 2017

BioMed Research International

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Porcine circovirus virus type II (PCV2) is the etiology of postweaning multisystemic wasting syndrome (PMWS), porcine dermatitis, nephropathy syndrome (PDNS), and necrotizing pneumonia. Rapid diagnosis tool for detection of PCV2 plays an important role in the disease control and eradication program. Recombinase polymerase amplification (RPA) assays using a real-time fluorescent detection (PCV2 real-time RPA assay) and RPA combined with lateral flow dipstick (PCV2 RPA LFD assay) were developed targeting the PCV2 ORF2 gene. The results showed that the sensitivity of the PCV2 real-time RPA assay was 102 copies per reaction within 20 min at 37°C and the PCV2 RPA LFD assay had a detection limit of 102 copies per reaction in less than 20 min at 37°C. Both assays were highly specific for PCV2, with no cross-reactions with porcine circovirus virus type 1, foot-and-mouth disease virus, pseudorabies virus, porcine parvovirus, porcine reproductive and respiratory syndrome virus, and classical swine fever virus. Therefore, the RPA assays provide a novel alternative for simple, sensitive, and specific identification of PCV2.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2

Yang

Qin

Sun

et al. 2017

BioMed Research International

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“…Systems using LAMP, RPA, and NASBA have shown promising test characteristics in laboratory settings detecting a full range of infectious causes of acute febrile illness including Ebola, Zika, influenza, and scrub typhus ( Table 2). 93,105,106,115 Reagents and an instrument to perform RPA are commercially available from twistDx (Cambridge, United Kingdom), which have worked effectively for the field diagnosis of dengue. 107 The most mature applications of LAMP use an instrument with an isothermal heat source and real-time fluorometric or turbidimetric detection, available from multiple manufacturers.…”

Section: Traditional Microbiology Techniques Reinvent Themselvesmentioning

confidence: 99%

Reducing Uncertainty for Acute Febrile Illness in Resource-Limited Settings: The Current Diagnostic Landscape

Robinson

Manabe

2017

The American Society of Tropical Medicine and Hygiene

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Diagnosing the cause of acute febrile illness in resource-limited settings is important-to give the correct antimicrobials to patients who need them, to prevent unnecessary antimicrobial use, to detect emerging infectious diseases early, and to guide vaccine deployment. A variety of approaches are yielding more rapid and accurate tests that can detect more pathogens in a wider variety of settings. After decades of slow progress in diagnostics for acute febrile illness in resource-limited settings, a wave of converging advancements will enable clinicians in resource-limited settings to reduce uncertainty for the diagnosis of acute febrile illness.

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“…For the CaPV RPA LFD assay developed in this study, only a water bath is required for the RPA assay itself, and the amplicons analysis could be performed simply on the LFD strip which can be read by the naked eyes. A simple point-of-care scanner (ESEQuant tube scanner device, Germany) can also be used in the real-time RPA assay [13, 14]. The scanner is much cheaper and simpler than thermal cycler machine, and is also powered by battery which can be changed in the field.…”

Section: Discussionmentioning

confidence: 99%

“…As an alternative to real-time detection, RPA amplicons could also be visualized on lateral flow dipstick with TwistAmp nfo probe. Currently, RPA assay has been successfully developed for rapid detection of different viruses of veterinary importance, including foot-and-mouth disease virus [13], avian influenza H5N1 [14], Orf virus [15, 16], bovine viral diarrhea virus [17] and canine parvovirus type 2 [18]. The purpose of this study was to develop a RPA assay based on either real-time fluorescent detection (real-time RPA assay) or lateral flow dipstick (RPA LFD assay) for rapid detection of GTPV and SPPV and evaluate its performance in clinical samples.…”

Section: Introductionmentioning

confidence: 99%

Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus

Yang

Qin

Zhang

et al. 2017

Virol J

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BackgroundGoatpox virus (GTPV) and sheeppox virus (SPPV), which belong to the Capripoxvirus (CaPV), are economically important pathogens of small ruminants. Therefore, a sensitive, specific and rapid diagnostic assay for detection of GTPV and SPPV is necessary to accurately and promptly control these diseases.MethodsRecombinase polymerase amplification (RPA) assays combined with a real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the CaPV G-protein-coupled chemokine receptor (GPCR) gene, respectively.ResultsThe sensitivity of both CaPV real-time RPA assay and CaPV RPA LFD assay were 3 × 102 copies per reaction within 20 min at 38 °C. Both assays were highly specific for CaPV, with no cross-reactions with peste des petits ruminants virus, foot-and-mouth disease virus and Orf virus. The evaluation of the performance of these two assays with clinical sample (n = 107) showed that the CaPV real-time RPA assay and CaPV RPA LFD assay were able to specially detect SPPV or GTPV present in samples of ovine in liver, lung, kidney, spleen, skin and blood.ConclusionsThis study provided a highly time-efficient and simple alternative for rapid detection of GTPV and SPPV.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-017-0792-7) contains supplementary material, which is available to authorized users.

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Development of reverse transcription recombinase polymerase amplification assay for avian influenza H5N1 HA gene detection

Cited by 38 publications

References 31 publications

Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2

Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2

Reducing Uncertainty for Acute Febrile Illness in Resource-Limited Settings: The Current Diagnostic Landscape

Development of real-time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus

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