Development of reversible bonding for microfluidic applications

Dinh, Thi Hong Nhung; Cao, Hong; Hamdi, Feriel; Couty, Magdalèna; Martincic, Émile; Woytasik, Marion; Dufour-Gergam, Elisabeth

doi:10.1007/s10404-015-1599-8

Cited by 8 publications

(5 citation statements)

References 23 publications

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“…Channel cap (3.5 cm long, 500 μm wide, 100 μm high, with 6 mm diameter of reservoir at the middle, V ∼3 μL) was fabricated in PDMS by replica molding process . Bottom substrate was prepared by following steps: (i) a 100 μm thick layer of PDMS was spin‐coated over a 2 inches glass substrate.…”

Section: Methodssupporting

confidence: 91%

“…This PDMS layer helps the bonding process of channel cap to bottom substrate. (iii) The PDMS channel cap was bonded to the bottom substrate by stamping technique that is a reversible bonding method . In this step, a 100 μm PDMS layer was used instead of DMPMS (dimethyl‐methylphenylmethoxy siloxane) as the “glue” inter‐layer (reported in ).…”

Section: Methodsmentioning

confidence: 92%

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Sodium Dodecyl Sulfate Doped Polyaniline for Enhancing the Electrochemical Sensitivity of Mercury Ions

et al. 2016

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Polyaniline (PANi) was electro‐synthesized on the surface of screen‐printed carbon electrodes in the presence of sodium dodecyl sulfate (SDS) as a dopant. The complex of aniline and SDS created a conductive (PANi‐SDS) film at lower aniline concentration. The PANi‐SDS film contained negative charge due to the anionic head of SDS. The PANi‐SDS modified electrode was integrated into a poly(dimethylsiloxane) microfluidic chip as an electrochemical sensor for mercury detection. The presence of SDS in the polyaniline film enhanced the possibility of mercury ions uptake, and therefore, increased the peak current of square wave anodic stripping in the mercury detection. The mercury sensor exhibited a dynamic range from 6 to 35 nM with detection limit of 2.4 nM.

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Section: Methodssupporting

confidence: 91%

Section: Methodsmentioning

confidence: 92%

Sodium Dodecyl Sulfate Doped Polyaniline for Enhancing the Electrochemical Sensitivity of Mercury Ions

et al. 2016

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“…Additionally, oxygen plasma followed by chemical treatment was also applied to other materials to enhance the bonding strength of PDMS-based devices. 27 For instance, plasma-activated PDMS can bond to a PS substrate with a weak bonding strength of around 120 mbar. However, irreversible bonding can be achieved by treating the PS substrate with 1% APTES solution after oxygen plasma treatment.…”

Section: Reduced Aptes Concentration Facilitates Reversible Bonding B...mentioning

confidence: 99%

Reversibly-bonded microfluidic devices for stable cell culture and rapid, gentle cell extraction

Feng,

Wu,

Cheng

et al. 2024

Lab Chip

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“…Additionally, oxygen plasma followed by chemical treatment was also applied to other materials to enhance the bonding strength of PDMS-based devices. [21] For instance, plasma-activated PDMS can bond to a PS substrate with a weak bonding strength of around 1.72 psi. However, irreversible bonding can be achieved by treating the PS substrate with 1% APTES solution after oxygen plasma treatment.…”

Section: Reduced Aptes Concentration Facilitates Reversible Bonding B...mentioning

confidence: 99%

Reversibly-bonded Microfluidic Devices for Stable Cell Culture and Rapid, Gentle Cell Extraction

Feng,

Wai Cheng,

Lin

et al. 2023

Preprint

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Microfluidics chips have emerged as significant tools in cell culture due to their capacity for supporting cells to adopt more physiologically relevant morphology in 3D compared with traditional cell culture in 2D. Currently, irreversible bonding methods commonly used in chip fabrication mean that chips cannot be detached from their substrate without destroying the chip structure, which makes it challenging to do further analysis on cells that have been cultured on-chip. Some reversible bonding techniques exist but are restricted to certain materials, or require complex processing procedures. Here, we demonstrate a simple and reversible polydimethylsiloxane (PDMS)-polystyrene (PS) bonding technique that allows devices to withstand extended operation while pressurized, and supports long-term stable cell cultures. Importantly, it allows rapid and gentle live cell extraction for further downstream manipulation and characterization after long-term on-chip culturing, or even further subculturing. Our new approach could greatly facilitate microfluidic chip-based tissue and cell cultures, overcoming current analytical limitations and opening up new avenues for downstream uses of on-chip cultures, including 3D-engineered tissue structures for biomedical applications.

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Development of reversible bonding for microfluidic applications

Cited by 8 publications

References 23 publications

Sodium Dodecyl Sulfate Doped Polyaniline for Enhancing the Electrochemical Sensitivity of Mercury Ions

Sodium Dodecyl Sulfate Doped Polyaniline for Enhancing the Electrochemical Sensitivity of Mercury Ions

Reversibly-bonded microfluidic devices for stable cell culture and rapid, gentle cell extraction

Reversibly-bonded Microfluidic Devices for Stable Cell Culture and Rapid, Gentle Cell Extraction

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