Development of RFLP-PCR and simple multiplex PCR assays for detection and differentiation of two species of hemotropic mycoplasmas in naturally infected dogs

Sharifiyazdi, Hassan; Hasiri, Mohammad Abbaszadeh; Radmanesh, Mahshid

doi:10.1007/s00580-016-2272-7

Cited by 4 publications

(3 citation statements)

References 19 publications

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“…in dogs [ 7 ]. However, the prevalence of hemoplasmosis in Saudi Arabia as determined in the current study is similar to that recorded in Iran, where 22–25.4% cats and 6.25–22% of dogs with single or multiple hemoplasmas have been reported [ 33 , 34 , 35 , 36 , 37 ]. Statistical analyses revealed that older dogs were infected more often than younger ones.…”

Section: Discussionsupporting

confidence: 86%

Molecular Survey of Vector-Borne Pathogens of Dogs and Cats in Two Regions of Saudi Arabia

et al. 2020

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Dogs and cats play an important role as reservoirs of vector-borne pathogens, yet reports of canine and feline vector-borne diseases in Saudi Arabia are scarce. Blood samples were collected from 188 free-roaming dogs and cats in Asir (70 dogs and 44 cats) and Riyadh (74 dogs), Saudi Arabia. The presence of Anaplasma spp., Bartonella spp., hemotropic Mycoplasma spp., Babesia spp., and Hepatozoon spp. was detected using a multiplex tandem real-time PCR. PCR-positive samples were further examined with specific conventional and real-time PCR followed by sequencing. Dogs from Riyadh tested negative for all pathogens, while 46 out of 70 dogs (65.7%) and 17 out of 44 cats (38.6%) from Asir were positive for at least one pathogen. Positive dogs were infected with Anaplasma platys (57.1%), Babesia vogeli (30%), Mycoplasma haemocanis (15.7%), and Bartonella henselae (1.4%), and cats were infected with Mycoplasma haemofelis (13.6%), Candidatus Mycoplasma haemominutum (13.6%), B. henselae (9.2%), and A. platys (2.27%), all of which are reported for the first time in Saudi Arabia. Co-infection with A. platys and B. vogeli was detected in 17 dogs (24.28%), while coinfections were not detected in cats. These results suggest that effective control and public awareness strategies for minimizing infection in animals are necessary.

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Section: Discussionsupporting

confidence: 86%

Molecular Survey of Vector-Borne Pathogens of Dogs and Cats in Two Regions of Saudi Arabia

et al. 2020

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“…Since the rst record of CMhp in a dog in southern city of Shiraz 28 few molecular-based studies have been performed. In Shiraz, 6 (6.0%) and 4 (4.0%) of 100 examined dogs were positive for Mhc and CMhp with no case of concurrent infection 27 . In another study in the same city, Mhc and CMhp were detected in 4 (7.5%) and 3 (5.7%) out of 53 dogs 30 .…”

Section: Discussionmentioning

confidence: 95%

“…Canine hemoplasmas have been reported from many regions including the Middle East such as Turkey 22 , Qatar 23 , Saudi Arabia 24 and Egypt 25 however, there is shortage of information about these parasites in Iran as in the previous studies, limited number of dogs from two cities (i.e. Shiraz and Esfahan) were examined 26,27 . The purpose of the present study was to determine the prevalence and molecular characterization of hemotropic Mycoplasma species in apparently healthy dogs and their ectoparasites from ve Iranian provinces with different climates.…”

Section: Introductionmentioning

confidence: 99%

Molecular detection and identification of hemotropic Mycoplasma species in dogs and their ectoparasites in Iran

Beus,

Goudarztalejerdi,

Sazmand

2023

Preprint

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Hemotropic Mycoplasma species are vector-borne bacteria that attach and grow on the surface of erythrocytes in various mammals, yet reports of canine hemoplasmosis in Iran are scarce. The aim of this study was molecular detection and identification of hemoplasmas in the blood of dogs (n = 370) from five provinces of Iran and ectoparasites infesting them including Ctenocephalides canis and Pulex irritans fleas, Rhipicephalus sanguineus sensu lato ticks, Heterodoxus spiniger lice and Hippobosca longipennis keds. The presence of hemotropic Mycoplasma spp. pathogens were detected using group-specific, and identified using species-specific conventional PCRs detecting Mycoplasma haemocanis (Mhc) and Candidatus Mycoplasma haematoparvum (CMhp) followed by Sanger sequencing. Correlation of infection and risk factors (geographical area, keeping condition, body condition, sex, age, infestation with ectoparasite) were analyzed. In total, 210 dogs (56.7%) tested PCR-positive for hemotropic Mycoplasma spp. Species-specific PCR and sequencing revealed infection with Mhc in 17.8%, with CMhp in 7.02% and co-infection in 31.9% of dogs. Flea infestation, poor body condition, and being older than 3-years-old correlated with hemoplasmosis. In ectoparasites, DNA of hemoplasmas were detected only in fleas i.e. Mhc in P. irritans, CMhp in P. irritans and C. canis, and co-infection with Mhc an CMhp in C. canis. To our knowledge, this is the first large-scale molecular epidemiology study of canine hemoplasmosis in Iran. Considering the high prevalence of canine hemoplasmosis all over the country including potentially zoonotic CMhp, effective ectoparasite control strategies, regular examination of dogs, successful chemoprophylaxis and public awareness strategies are advocated.

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Occurrence of canine hemotropic mycoplasmas in domestic dogs from urban and rural areas of the Valdivia Province, southern Chile

Soto

Walker

Sepúlveda³

et al. 2017

Comparative Immunology, Microbiology and Infectious Diseases

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Development of RFLP-PCR and simple multiplex PCR assays for detection and differentiation of two species of hemotropic mycoplasmas in naturally infected dogs

Cited by 4 publications

References 19 publications

Molecular Survey of Vector-Borne Pathogens of Dogs and Cats in Two Regions of Saudi Arabia

Molecular Survey of Vector-Borne Pathogens of Dogs and Cats in Two Regions of Saudi Arabia

Molecular detection and identification of hemotropic Mycoplasma species in dogs and their ectoparasites in Iran

Occurrence of canine hemotropic mycoplasmas in domestic dogs from urban and rural areas of the Valdivia Province, southern Chile

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