Development of Rhizoctonia solani on Stems, Stolons and Tubers of Potatoes I. Effect of Inoculum Source

Atkinson, Dennis; Thornton, Michael K.; Miller, Jeffery S.

doi:10.1007/s12230-010-9143-6

Cited by 26 publications

(31 citation statements)

References 16 publications

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“…Several studies have been conducted to investigate the influence of inoculum source of R. solani on potato disease development (Atkinson et al 2010;Brierley et al 2016;Carling et al 1989;Tsror (Lahkim) and Peretz-Alon 2005). However, these studies differ as to which inoculum source is more important for causing Rhizoctonia disease epidemics on potato.…”

Section: Discussionmentioning

confidence: 99%

“…The AG identity of the isolates was confirmed by DNA sequencing of the internal transcribed spacer region (ITS) as described in a separate study (Muzhinji et al 2015). Soil-borne inoculum was prepared according to the method of Atkinson et al (2010) homogenized by blending in 4 L of sterile water into slurry, mixed with 10 L of vermiculite and incubated for five weeks with shaking after every two days. Soil was inoculated two days prior to planting by spreading the Rhizoctonia colonised vermiculite throughout the potato field at an approximate rate of 2 L per 9 m of row.…”

Section: Inoculum Preparationmentioning

confidence: 99%

“…The role of seed tuber-and soil-borne inocula in the development of Rhizoctonia diseases on potatoes has been extensively studied but the relative importance of each source of inoculum has not been definitively established (Atkinson et al 2010;Brierley et al 2016;Frank and Leach 1980;Tsror (Lahkim) and Peretz-Alon 2005;van Emden et al 1965). James and Mckenzie (1972) demonstrated that soil-borne inoculum is more important than seed tuber-borne inoculum, while Atkinson et al (2010) and more recently Brierley et al (2016) concluded that seed tuber-borne is more strongly associated with potato diseases than soilborne inoculum.…”

Section: Introductionmentioning

confidence: 99%

“…James and Mckenzie (1972) demonstrated that soil-borne inoculum is more important than seed tuber-borne inoculum, while Atkinson et al (2010) and more recently Brierley et al (2016) concluded that seed tuber-borne is more strongly associated with potato diseases than soilborne inoculum. In contrast, other studies have shown that seed tuber-and soil-borne inocula are both important in potato disease development with each source of inoculum playing a different role at different stages of plant growth and development (Atkinson et al 2010;Brierlery et al 2016;Frank and Leach 1980;Tsror (Lahkim) and Peretz-Alon 2005).…”

Section: Introductionmentioning

confidence: 99%

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Relative Contribution of Seed Tuber- and Soilborne Inoculum to Potato Disease Development and Changes in the Population Genetic Structure of Rhizoctonia solani AG 3-PT under Field Conditions in South Africa

et al. 2018

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Understanding the contribution of seed tuber-and soil-borne inocula of Rhizoctonia solani AG 3-PT in causing potato disease epidemics is an important step in implementing effective management strategies for the pathogen. A two-year study was conducted to evaluate the contribution of each source of inoculum using an integrative experimental approach combining field trials and molecular techniques. Two distinct sets of genetically-marked isolates were used as seed tuber-borne and soil-borne inocula in a mark-release-recapture experiment. Disease assessments were done during tuber initiation and at tuber harvest Both inoculuma sources were found to be equally important in causing black scurf disease, whereas soil-borne inocula appeared to be more important for root and stolon infection, and seed-borne inocula contributed more to stem canker. However, seed tuber transmitted genotypes accounted for 60% of the total recovered isolates when genotyped using three PCR-RFLP markers. The changes in population structure of the experimental 1 R. solani population over the course of the growing season and across two growing seasons were investigated using eight microsatellite markers. The populations at different sampling times were somewhat genetically differentiated as indicated by Nei's gene diversity (0.24 -0.27) and the fixation index (F ST ) . The proportion of isolates with genotypes that differed from the inoculants ranged from 13% to 16% in 2013 and 2014, respectively, suggesting the possibility of emergence of new genotypes in the field. Because both soilborne and tuber-borne inocula are critical, it is important to ensure the use of pathogen-free seed tubers to eliminate seed tuber-borne inoculum and the introduction of new genotypes of R. solani for sustainable potato production in South Africa.

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Section: Discussionmentioning

confidence: 99%

Section: Inoculum Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Relative Contribution of Seed Tuber- and Soilborne Inoculum to Potato Disease Development and Changes in the Population Genetic Structure of Rhizoctonia solani AG 3-PT under Field Conditions in South Africa

et al. 2018

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“…Soil inoculum was prepared by growing an isolate of R. solani recovered from a potato stem lesion on potato dextrose agar (PDA) as previously described (Atkinson et al 2010). R. solani cultures containing agar and mycelia fragments were blended with water into a slurry and mixed with vermiculite to create the soil inoculum.…”

Section: Methodsmentioning

confidence: 99%

Development of Rhizoctonia solani on Stems, Stolons and Tubers of Potato II. Efficacy of Chemical Applications

Atkinson¹,

Thornton

Miller

2010

Am. J. Pot Res

Self Cite

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Fungicide treatments have proven successful in reducing Rhizoctonia solani lesions on stems and stolons; as well as black scurf on tubers. Field studies in Parma and Aberdeen, Idaho, were conducted in 2004 and 2005 to compare the relative effectiveness of in-furrow and seed treatments for disease control on stems, stolons and tubers. The effectiveness of each fungicide placement was evaluated for control of seed-borne and soil-borne inoculum. The systemic fungicide azoxystrobin was used as the in-furrow fungicide treatment and fludioxonil was used as the seed treatment. A combination of seed treatment with an in-furrow treatment was also evaluated. All fungicide treatments had significantly lower stem and stolon disease ratings than did the non-treated control. The seed plus in-furrow treatment tended to reduce stem and stolon disease ratings more than in-furrow treatment alone. Black scurf ratings on daughter tubers were significantly reduced by fungicide applications compared to the non-treated control in two of three trials. The combination treatment had lower black scurf ratings than the in-furrow treatment alone. There were more significant differences among fungicide treatments in Rhizoctonia solani lesions on stems, stolons and tubers when seed inoculum was moderate to high compared to low seed inoculum. In-furrow treatment was relatively more effective in reducing disease on stems, stolons and tubers when soil was the primary source of inoculum, while seed treatment was relatively more effective when seed was the primary inoculum source.Resumen Los tratamientos con fungicidas han probado ser exitosos en la reducción de las lesiones en tallos y estolones por Rhizoctonia solani; así como de las costras negras en tubérculos. En 2004 y 2005 se condujeron estudios en Parma y Aberdeen, Idaho, para comparar la efectividad relativa de tratamientos a la semilla y al surco para el control de la enfermedad en tallos, estolones y tubérculos. Se evaluó la efectividad de cada colocación del fungicida para controlar el inóculo proveniente de semilla y suelo. El fungicida sistémico azoxystrobin se usó como tratamiento de fungicida al surco y fludioxonil como tratamiento a la semilla. También se evaluó un tratamiento que combinó la aplicación a la semilla y al surco. Todos los tratamientos fungicidas tuvieron niveles de enfermedad en tallo y estolón significativamente más bajos que el testigo sin tratamiento. El tratamiento a la semilla y al surco tuvo la tendencia de reducir los niveles de enfermedad en tallo y estolón más que el del surco solamente. Las categorías de la costra negra en los tubérculos hijos se redujo significativamente con las aplicaciones del fungicida en comparación con el testigo sin tratar en dos de los tres ensayos. El tratamiento combinado tuvo niveles más bajos de costra negra que el tratamiento solo al surco. Hubo más diferencias significativas entre los tratamientos de fungicidas en las lesiones de tallos, estolones y tubérculos por Rhizoctonia solani con moderado a alto inóculo en la semilla, c...

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Bioactive Streptomycetes from Isolation to Applications: A Tasmanian Potato Farm Example

Ashfield-Crook¹,

Woodward²,

Soust³

et al. 2020

The Plant Microbiome

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Development of Rhizoctonia solani on Stems, Stolons and Tubers of Potatoes I. Effect of Inoculum Source

Cited by 26 publications

References 16 publications

Relative Contribution of Seed Tuber- and Soilborne Inoculum to Potato Disease Development and Changes in the Population Genetic Structure of Rhizoctonia solani AG 3-PT under Field Conditions in South Africa

Relative Contribution of Seed Tuber- and Soilborne Inoculum to Potato Disease Development and Changes in the Population Genetic Structure of Rhizoctonia solani AG 3-PT under Field Conditions in South Africa

Development of Rhizoctonia solani on Stems, Stolons and Tubers of Potato II. Efficacy of Chemical Applications

Bioactive Streptomycetes from Isolation to Applications: A Tasmanian Potato Farm Example

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