Development of rodlet cells in the gut of turbot (Psetta maxima L.): Relationship between their morphology and S100 protein immunoreactivity

Vigliano, Fabricio Andrés; Bermúdez, Roberto; Nieto, J. M.; Quiroga, María Isabel

doi:10.1016/j.fsi.2008.02.016

Cited by 28 publications

(25 citation statements)

References 33 publications

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“…We described three cell forms that are likely to be phases in the life cycle of RCs. In the examined species, the main morphological characteristics of different developmental stages of RCs resembled to those described by other authors (Bielek,2005; Leino,1974; Kramer and Potter,2002; Vigliano et al,2009), although some differences have been found.…”

Section: Discussionsupporting

confidence: 85%

“…In our study, as well as in the earlier mentioned, it appeared that at a very early stage of the development, the immature RCs were characterized by the presence of those organelles that indicated an intense secretory activity (Bielek,2005; Kramer and Potter,2002; Leino,1974; Vigliano et al,2009). We considered these cells, although lacking rodlets, to be RCs at an earliest stage in development, because of their morphological resemblance with the adjacent immature RCs, which displayed developing rodlets.…”

Section: Discussionsupporting

confidence: 80%

“…As a consequence, a longstanding controversy exists in the literature regarding the origin and nature of RCs. Although the exogenous hypothesis considers them as parasites (Bannister,1966; Barber,1979; Bielek and Viehberger,1983; Mayberry et al,1979; Richards et al,1994; Viehberger and Bielek,1982), the endogenous hypothesis regards them as normal constituent of fishes animal tissue, with a potential secretory activity (Bielek,2005; Dezfuli et al,1998, 2000, 2002,2003a, b; Leino,1974, 1982, 1996; Kramer and Potter,2002; Manera et al,2001; Mazon et al,2007; Vigliano et al,2006). Several evidence over recent years have supported the hypothesis that the RC participates in the fish non‐specific immune response (Abraham et al,2001; Dezfuli et al,1998, 2000, 2003a; Leino,1996; Reite,1997; Reite and Evensen,2006 ; Sulimanovic et al,1996).…”

Section: Introductionmentioning

confidence: 99%

“…However, whatever they could be, RCs seem to be involved in the response to disease and environmental insult. To shed more light on the longstanding controversy about the origin and function of RC and because scarce data are present about the cell development in the intestinal tract of teleost fishes (Bielek,2005; Vigliano et al,2009), we analyzed by transmission electron microscopy the ultrastructural characteristics during RC development in intestine of the teleost Dicentrarchus Labrax . The aim of this work is to characterize the phases in the life cycle of RCs in the intestine of the sea bass to add new insights on the morphology as well as on the function linked to the immunity system of this enigmatic cells.…”

Section: Introductionmentioning

confidence: 99%

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Rodlet cells development in the intestine of sea bass (Dicentrarchus labrax)

Laurà

Germanà

Levanti

et al. 2012

Microscopy Res & Technique

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The enigmatic rodlet cells (RCs) are characterized by conspicuous inclusions named "rodlets". They were discovered over 100 years ago and were considered as parasites but shortly afterward interpreted as endogenous cells. The RCs have been described in different tissues of marine and freshwater teleosts, but their origin and function remain unknown. This work was designed to an ultrastructural study on RCs development and distribution in intestinal epithelium of Dicentrarchus labrax. Three different stages of RCs development from early precursor cells to mature phase were observed, as well as a migration and finally an extrusion of their contents. In this study, the immature cells were found near the basal epithelium membrane. They were mainly identified by a rough endoplasmic reticulum with dilated cisternae, by developing rodlets and a thin fibrillar coat. The maturing RCs, localized in the middle zone of the epithelium, appeared to be undergoing a reorganization of the cell organelles. The mature RCs, placed near the free surface, showed a thick subplasmalemmar fibrillar coat. Most of the organelles were aggregated at the cell apex with a basally located nucleus. A cellular polarity was more evident. One of the most conspicuous features was the occurrence of mature rodlets club-sac in shape orientated toward the cell apex. Adhesive junctions between surface epithelial cells and RCs, while discharging their contents, were seen. We have connected morphological figures and distribution to different stages of development in RCs, supporting the hypothesis of their secretory function.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Rodlet cells development in the intestine of sea bass (Dicentrarchus labrax)

Laurà

Germanà

Levanti

et al. 2012

Microscopy Res & Technique

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“…It is, therefore, reasonable to assume that the interactions with the paralogs p63 and p73 also occur in vivo. S100 proteins have been shown to be involved in differentiation processes and are usually expressed in a tissue-specific manner, and it is established that p63 and p73 are also involved in developmental processes (Fano et al, 1995;Zimmer et al, 1995;Ilg et al, 1996;Mills et al, 1999;Yang et al, 1999Yang et al, , 2000Chan et al, 2003;Donato, 2003;Buckiova and Syka, 2009;Vigliano et al, 2009). S100A2 has been shown to be a target gene of p63 and p73 (Lapi et al, 2006;Kirschner et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Molecular basis of S100 proteins interacting with the p53 homologs p63 and p73

et al. 2010

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S100 proteins modulate p53 activity by interacting with its tetramerization (p53TET, residues 325-355) and transactivation (residues 1-57) domains. In this study, we characterized biophysically the binding of S100A1, S100A2, S100A4, S100A6 and S100B to homologous domains of p63 and p73 in vitro by fluorescence anisotropy, analytical ultracentrifugation and analytical gel filtration. We found that S100A1, S100A2, S100A4, S100A6 and S100B proteins bound different p63 and p73 tetramerization domain variants and naturally occurring isoforms with varying affinities in a calcium-dependent manner. Additional interactions were observed with peptides derived from the p63 and p73 N-terminal transactivation domains. Importantly, S100 proteins bound p63 and p73 with different affinities in their different oligomeric states, similarly to the differential modes of binding to p53. On the basis of our data, we hypothesize that S100 proteins regulate the oligomerization state of all three p53 family members and their isoforms, with a potential physiological relevance in developmental and disease-related processes. The regulation of the p53 family by S100 is complicated and depends on the target preference of each individual S100 protein, the concentration of the proteins and calcium, as well as the splicing variation of p63 or p73. Our results outlining the complexity of the interaction should be considered when studying the functional effects of S100 proteins in their biological context.

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Multi-organ histological observations on juvenile Senegalese soles exposed to low concentrations of waterborne cadmium

Costa

Caeiro

Costa

2012

Fish Physiol Biochem

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A histopathological screening was performed on juvenile Senegalese soles exposed to environmentally realistic concentrations of waterborne Cd (0.5, 5 and 10 μg L(-1)) for 28 days. The severity and dissemination of histopathological changes were variable and limited to the kidney, liver, spleen, gills and skin goblet cells. Contradicting available literature that refers the liver as the most affected organ upon acute exposure and the kidney following chronic exposure, the liver was the most impacted organ (even at the lowest concentration), in a trend that could relate to the duration of exposure and Cd concentration. The most noticeable hepatic alterations related to inflammation, although hepatocellular alterations like lipidosis and eosinophilic foci also occurred. The trunk kidney of exposed fish endured moderate inflammation, apoptosis and necrosis, however, without a clear time-dependent effect. The spleen of fish subjected to the highest concentrations revealed diffuse necrotic foci accompanied by melanomacrophage intrusion. The gills, albeit the most important apical uptake organ of dissolved toxicants, sustained only moderate damage, from epithelial hyperplasia and pavement cell detachment to the potentially more severe chloride cell alterations. In the skin, an increase in goblet cell size occurred, most notoriously correlated to Cd concentration at earlier stages of exposure. The results show that a metal-naïve juvenile fish can endure deleterious effects when exposed to low, ecologically relevant, concentrations of a common toxic metal and that the pattern of Cd-induced histopathological alterations can be complex and linked to organ-specific responses and metal translocation within the organism.

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Development of rodlet cells in the gut of turbot (Psetta maxima L.): Relationship between their morphology and S100 protein immunoreactivity

Cited by 28 publications

References 33 publications

Rodlet cells development in the intestine of sea bass (Dicentrarchus labrax)

Rodlet cells development in the intestine of sea bass (Dicentrarchus labrax)

Molecular basis of S100 proteins interacting with the p53 homologs p63 and p73

Multi-organ histological observations on juvenile Senegalese soles exposed to low concentrations of waterborne cadmium

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