Development of rolling circle amplification based surface-enhanced Raman spectroscopy method for 35S promoter gene detection

Güven, Burcu; Boyaci, İsmail Hakkı; Tamer, Uğur; Acar-Soykut, Esra; Dogan, Üzeyir

doi:10.1016/j.talanta.2014.11.051

Cited by 22 publications

(9 citation statements)

References 51 publications

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“…In this work, the accuracy and sensitivity of the proposed SERS-based method was comparable with real-time PCR. The 35S promoter gene, a marker of a genetically modified organism, has been detected by SERS-based rolling circle amplification, with an LOD of 6.3 fM and detection range from 100 fM to 100 nM [40]. The rolling circle amplification (RCA) reaction was utilized to develop an RCA-SERS sandwich assay for enhancing the SERS spectra of target molecules.…”

Section: Sers For Dna Detectionmentioning

confidence: 99%

Novel Surface-Enhanced Raman Spectroscopy Techniques for DNA, Protein and Drug Detection

Chen

Liu

Tian

et al. 2019

Sensors

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Surface-enhanced Raman spectroscopy (SERS) is a vibrational spectroscopic technique in which the Raman scattering signal strength of molecules, absorbed by rough metals or the surface of nanoparticles, experiences an exponential growth (103–106 times and even 1014–1015 times) because of electromagnetic or chemical enhancements. Nowadays, SERS has attracted tremendous attention in the field of analytical chemistry due to its specific advantages, including high selectivity, rich informative spectral properties, nondestructive testing, and the prominent multiplexing capabilities of Raman spectroscopy. In this review, we present the applications of state-of-the-art SERS for the detection of DNA, proteins and drugs. Moreover, we focus on highlighting the merits and mechanisms of achieving enhanced SERS signals for food safety and clinical treatment. The machine learning techniques, combined with SERS detection, are also indicated herein. This review concludes with recommendations for future studies on the development of SERS.

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Section: Sers For Dna Detectionmentioning

confidence: 99%

Novel Surface-Enhanced Raman Spectroscopy Techniques for DNA, Protein and Drug Detection

Chen

Liu

Tian

et al. 2019

Sensors

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“…This is based on DNA isothermal polymerization performed by certain enzymes whose activity can be triggered by binding events. Currently, this approach is typically exploited in sandwich assays in conjunction with NPs for colorimetric tests in solution [95] or with nano-rods for SERS detection [96] on a surface. Although, in principle, this kind of amplification can tremendously improve sensitivity (down to fg/ml), no real label-free detection has yet been demonstrated.…”

Section: Dna-based Amplification Strategiesmentioning

confidence: 99%

Emerging applications of label-free optical biosensors

et al. 2017

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Abstract:Innovative technical solutions to realize optical biosensors with improved performance are continuously proposed. Progress in material fabrication enables developing novel substrates with enhanced optical responses. At the same time, the increased spectrum of available biomolecular tools, ranging from highly specific receptors to engineered bioconjugated polymers, facilitates the preparation of sensing surfaces with controlled functionality. What remains often unclear is to which extent this continuous innovation provides effective breakthroughs for specific applications. In this review, we address this challenging question for the class of label-free optical biosensors, which can provide a direct signal upon molecular binding without using secondary probes. Label-free biosensors have become a consolidated approach for the characterization and screening of molecular interactions in research laboratories. However, in the last decade, several examples of other applications with high potential impact have been proposed. We review the recent advances in label-free optical biosensing technology by focusing on the potential competitive advantage provided in selected emerging applications, grouped on the basis of the target type. In particular, direct and real-time detection allows the development of simpler, compact, and rapid analytical methods for different kinds of targets, from proteins to DNA and viruses. The lack of secondary interactions facilitates the binding of small-molecule targets and minimizes the perturbation in single-molecule detection. Moreover, the intrinsic versatility of label-free sensing makes it an ideal platform to be integrated with biomolecular machinery with innovative functionality, as in case of the molecular tools provided by DNA nanotechnology.

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“…[39][40][41] Although most of them have demonstrated their potential with synthetic oligonucleotides, as a proof of concept, the jump to the real world by using certified reference materials remains mostly unexplored. Different signal amplification approaches [42][43][44][45] have been described achieving sensitivities nM-pM which, in a few cases, have been challenged with food samples, reporting successful results difficult to explain taking into account the sensitivity of the described tests. 42,45 Considering the sensitivity of the electrochemical genosensor here developed (detection limit in the low picomolar range), we decided to test it for direct detection of GM-maize extracted DNA without DNA amplification.…”

Section: Genosensor Design and Analytical Performancementioning

confidence: 99%

Targeting Helicase-Dependent Amplification Products with an Electrochemical Genosensor for Reliable and Sensitive Screening of Genetically Modified Organisms

Moura-Melo

Miranda‐Castro

de‐los‐Santos‐Álvarez

et al. 2015

Anal. Chem.

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Cultivation of genetically modified organisms (GMOs) and their use in food and feed is constantly expanding; thus, the question of informing consumers about their presence in food has proven of significant interest. The development of sensitive, rapid, robust, and reliable methods for the detection of GMOs is crucial for proper food labeling. In response, we have experimentally characterized the helicase-dependent isothermal amplification (HDA) and sequence-specific detection of a transgene from the Cauliflower Mosaic Virus 35S Promoter (CaMV35S), inserted into most transgenic plants. HDA is one of the simplest approaches for DNA amplification, emulating the bacterial replication machinery, and resembling PCR but under isothermal conditions. However, it usually suffers from a lack of selectivity, which is due to the accumulation of spurious amplification products. To improve the selectivity of HDA, which makes the detection of amplification products more reliable, we have developed an electrochemical platform targeting the central sequence of HDA copies of the transgene. A binary monolayer architecture is built onto a thin gold film where, upon the formation of perfect nucleic acid duplexes with the amplification products, these are enzyme-labeled and electrochemically transduced. The resulting combined system increases genosensor detectability up to 10(6)-fold, allowing Yes/No detection of GMOs with a limit of detection of ∼30 copies of the CaMV35S genomic DNA. A set of general utility rules in the design of genosensors for detection of HDA amplicons, which may assist in the development of point-of-care tests, is also included. The method provides a versatile tool for detecting nucleic acids with extremely low abundance not only for food safety control but also in the diagnostics and environmental control areas.

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Development of rolling circle amplification based surface-enhanced Raman spectroscopy method for 35S promoter gene detection

Cited by 22 publications

References 51 publications

Novel Surface-Enhanced Raman Spectroscopy Techniques for DNA, Protein and Drug Detection

Novel Surface-Enhanced Raman Spectroscopy Techniques for DNA, Protein and Drug Detection

Emerging applications of label-free optical biosensors

Targeting Helicase-Dependent Amplification Products with an Electrochemical Genosensor for Reliable and Sensitive Screening of Genetically Modified Organisms

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