Development of sandwich dot-ELISA for specific detection of Ochratoxin A and its application on to contaminated cereal grains originating from India

Mudili, Venkataramana; Ramakrishnan, Rashmi; Uppalapati, Siva R.; Siddaiah, Chandranayaka; Balakrishna, Keshava; Radhika, M.; Gupta, Vijai Kumar; Batra, H. V.

doi:10.3389/fmicb.2015.00511

Cited by 34 publications

(22 citation statements)

References 36 publications

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“…fumonisin (Makun et al 2011;Ortiz et al 2013;Rofiat et al 2015), aflatoxins (Nguyen et al 2007;Rahmani et al 2010;Aydin et al 2011;Makun et al 2011;Ortiz et al 2013;Lai et al 2014b;Rofiat et al 2015), but also beauvericin (Nazari et al 2015;Rofiat et al 2015), citrinin (Nguyen et al 2007;Rofiat et al 2015), patulin (Makun et al 2011), sterigmatocystin, moniliformin, fumonisin B 3 , cytochalasins (Rofiat et al 2015), enniatins (Nazari et al 2015), zearalenone (Rahmani et al 2010;Makun et al 2011;Lee et al 2015), deoxynivalenol (Dors et al 2011;Makun et al 2011;Ortiz et al 2013), and ochratoxin A (Pena et al 2005;Ghalia et al 2008;Juan et al, 2008;Frelund et al 2009;Buyukunal et al 2010;Rahmani et al 2010;Aydin et al 2011;Bansal et al 2011;Almeidaa et al 2012;Mosayebi & Mirzaee 2014;Rahimi 2014;Venkataramana et al 2015;Xianwen et al 2015). The rice parboiling process increases the migration of mineral salts by about 30% into parboiled rice (compared to white rice) during the soaking and boiling steps (Dors et al 2009).…”

mentioning

confidence: 99%

The occurrence of ochratoxin A in white and parboiled rice

Toman¹,

Malíř²,

Ostrý³

et al. 2016

Czech J. Food Sci.

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mentioning

confidence: 99%

The occurrence of ochratoxin A in white and parboiled rice

Toman¹,

Malíř²,

Ostrý³

et al. 2016

Czech J. Food Sci.

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“…The linear equation was y = 0.6294x + 0.753, with a correlation coefficient (R 2 ) of 0.8453 (Figure 4B). In the present study, the half inhibitory concentration (IC 50 ) was 0.2 ng/mL and the linear range to detect OTA was 0.06-0.6 ng/mL, which is defined as the concentration of OTA giving a 20-80% inhibition, and its lower detection limit (LOD) was 0.03 ng/mL, which was better, lower, than that reported in ELISA until now (Gyöngyösi-Horváth et al, 1996;Cho et al, 2005;Alarcón et al, 2006;Zhang et al, 2011Zhang et al, , 2015Pavón et al, 2012;Li et al, 2013;Venkataramana et al, 2015).…”

Section: Preparation Of Standard Curvementioning

confidence: 43%

Development of ELISA and Lateral Flow Immunoassays for Ochratoxins (OTA and OTB) Detection Based on Monoclonal Antibody

Fadlalla

Ling

Wang

et al. 2020

Front. Cell. Infect. Microbiol.

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Ochratoxins were important secondary metabolites secreted by fungi, and OTA and OTB are mainly significant mycotoxin, having toxic effects on humans and animals. Therefore, it is important to establish a rapid, sensitive, and precise method for ochratoxins detection and quantification in real samples. In this study, a stable monoclonal antibody (mAb) that recognizing both OTA and OTB toxins was employed for the establishment of indirect competitive ELISA (ic-ELISA), colloidal gold nanoparticles (CGNs), and nanoflowers gold strips (AuNFs) for detection of ochratoxins in real samples. A 6E5 hybridoma cell line stable secreting mAb against both OTA and OTB toxins was obtained by fusion of splenocytes with myeloma SP2/0 cells. The 6E5 mAb had a high affinity (3.7 × 10 8 L/mol) to OTA, and also showed similar binding activity to OTB. The optimized ic-ELISA resulted in a linear range of 0.06-0.6 ng/mL for ochratoxins (OTA and OTB) detection. The IC50 was 0.2 ng/mL and the limit of detection (LOD) was 0.03 ng/mL. The mean recovery rate from the spiked samples was 89.315 ± 2.257%, with a coefficient variation of 2.182%. The result from lateral flow immunoassays indicated that the LOD of CGNs and AuNFs were 5 and 1 µg/mL, respectively. All these results indicated that the developed ic-ELISA, CGNs, and AuNFs in this study could be used for the analysis of the residual of ochratoxins (OTA and OTB) in food and agricultural products.

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“…The majority of mycotoxins are soluble in polar solvents and insoluble in apolar solvents . The most common solvents that have been used in different studies for the extraction of mycotoxins from cereals are polar or relatively polar solvents, such as water, acetone, acetonitrile, methanol, chloroform or a mixture of them . In the case of fumonisins, an acidified solvent favours the protonation of carboxylic groups with a consequent improved separation from the matrix …”

Section: Analysis Of Mycotoxinsmentioning

confidence: 99%

Current methods for mycotoxins analysis and innovative strategies for their reduction in cereals: an overview

et al. 2018

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Mycotoxins are secondary metabolites produced by moulds in food that are considered a substantial issue in the context of food safety, due to their acute and chronic toxic effects on animals and humans. Therefore, new accurate methods for their identification and quantification are constantly developed in order to increase the performance of extraction, improve the accuracy of identification and reduce the limit of detection. At the same time, several industrial practices have shown the ability to reduce the level of mycotoxin contamination in food. In particular, a decrease in the amount of mycotoxins could result from standard processes naturally used for food processing or by procedures strategically introduced during processing, with the specific aim of reducing the amount of mycotoxins. In this review, the current methods adopted for accurate analyses of mycotoxins in cereals (aflatoxins, ochratoxins, trichothecenes, fumonisins) are discussed. In addition, both conventional and innovative strategies adopted to obtain safer finished products from common cereals intended for human consumption will be explored and analysed. © 2018 Society of Chemical Industry.

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Development of sandwich dot-ELISA for specific detection of Ochratoxin A and its application on to contaminated cereal grains originating from India

Cited by 34 publications

References 36 publications

The occurrence of ochratoxin A in white and parboiled rice

The occurrence of ochratoxin A in white and parboiled rice

Development of ELISA and Lateral Flow Immunoassays for Ochratoxins (OTA and OTB) Detection Based on Monoclonal Antibody

Current methods for mycotoxins analysis and innovative strategies for their reduction in cereals: an overview

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