Development of
            <scp>Aptamer‐Based</scp>
            Electrochemical Methods

Xu, Jianguo; Li, Yao; Cheng, Lin; Yan, Chao; Chen, Wei

doi:10.1002/9783527806799.ch9

Cited by 2 publications

(6 citation statements)

References 31 publications

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“…Aptamers (from the Latin word aptus (“fit”) and from the Greek word meros (“part”)), are essentially short and single-stranded DNA or RNA oligonucleotides, containing almost 20–80 nucleotides and with a molecular weight ranging from 6 to 30 kDa. They are able to interact specifically with a target molecule, similarly to the antigen–antibody interaction and consequently the aptamers are generally assumed as chemical antibodies [ 109 , 110 ]. The aptamers are selected from a large collection and assortment of nucleic acids, providing roughly 10 15 different sequences, using the well-known Systematic Evolution of Ligands by Exponential enrichment (SELEX) technology [ 109 , 110 ].…”

Section: Electrochemical Biosensors For Food Allergen Detectionmentioning

confidence: 99%

“…They are able to interact specifically with a target molecule, similarly to the antigen–antibody interaction and consequently the aptamers are generally assumed as chemical antibodies [ 109 , 110 ]. The aptamers are selected from a large collection and assortment of nucleic acids, providing roughly 10 15 different sequences, using the well-known Systematic Evolution of Ligands by Exponential enrichment (SELEX) technology [ 109 , 110 ]. Aptamers can make strong and specific interactions with a broad range of targets such as proteins, nucleotides, antibiotics, toxins, cancer cells, viruses, bacteria, and allergens.…”

Section: Electrochemical Biosensors For Food Allergen Detectionmentioning

confidence: 99%

“…Electrochemical aptasensors can be classified according to the adopted detection approach: labeled type where labels (antibodies, enzymes, metal nanoparticles, or redox compounds) are covalently or non-covalently linked to aptamers, or label-free type [ 109 ]. In both cases, the electrochemical response is correlated to the target concentration.…”

Section: Electrochemical Biosensors For Food Allergen Detectionmentioning

confidence: 99%

“…Aptamers (from the Latin word aptus ("fit") and from the Greek word meros ("part")), are essentially short and single-stranded DNA or RNA oligonucleotides, containing almost 20-80 nucleotides and with a molecular weight ranging from 6 to 30 kDa. They are able to interact specifically with a target molecule, similarly to the antigen-antibody interaction and consequently the aptamers are generally assumed as chemical antibodies [109,110].…”

Section: Aptasensorsmentioning

confidence: 99%

“…The aptamers are selected from a large collection and assortment of nucleic acids, providing roughly 10 15 different sequences, using the well-known Systematic Evolution of Ligands by Exponential enrichment (SELEX) technology [109,110]. Aptamers can make strong and specific interactions with a broad range of targets such as proteins, nucleotides, antibiotics, toxins, cancer cells, viruses, bacteria, and allergens.…”

Section: Aptasensorsmentioning

confidence: 99%

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Recent Advances in Electrochemical Sensing Strategies for Food Allergen Detection

Curulli

2022

Biosensors

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Food allergy has been indicated as the most frequent adverse reaction to food ingredients over the past few years. Since the only way to avoid the occurrence of allergic phenomena is to eliminate allergenic foods, it is essential to have complete and accurate information on the components of foodstuff. In this framework, it is mandatory and crucial to provide fast, cost-effective, affordable, and reliable analysis methods for the screening of specific allergen content in food products. This review reports the research advancements concerning food allergen detection, involving electrochemical biosensors. It focuses on the sensing strategies evidencing different types of recognition elements such as antibodies, nucleic acids, and cells, among others, the nanomaterial role, the several electrochemical techniques involved and last, but not least, the ad hoc electrodic surface modification approaches. Moreover, a selection of the most recent electrochemical sensors for allergen detection are reported and critically analyzed in terms of the sensors’ analytical performances. Finally, advantages, limitations, and potentialities for practical applications of electrochemical biosensors for allergens are discussed.

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Section: Electrochemical Biosensors For Food Allergen Detectionmentioning

confidence: 99%

Section: Electrochemical Biosensors For Food Allergen Detectionmentioning

confidence: 99%

Section: Electrochemical Biosensors For Food Allergen Detectionmentioning

confidence: 99%

Section: Aptasensorsmentioning

confidence: 99%

Section: Aptasensorsmentioning

confidence: 99%

See 3 more Smart Citations

Recent Advances in Electrochemical Sensing Strategies for Food Allergen Detection

Curulli

2022

Biosensors

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Functional Nanomaterials Enhancing Electrochemical Biosensors as Smart Tools for Detecting Infectious Viral Diseases

Curulli

2023

Molecules

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Electrochemical biosensors are known as analytical tools, guaranteeing rapid and on-site results in medical diagnostics, food safety, environmental protection, and life sciences research. Current research focuses on developing sensors for specific targets and addresses challenges to be solved before their commercialization. These challenges typically include the lowering of the limit of detection, the widening of the linear concentration range, the analysis of real samples in a real environment and the comparison with a standard validation method. Nowadays, functional nanomaterials are designed and applied in electrochemical biosensing to support all these challenges. This review will address the integration of functional nanomaterials in the development of electrochemical biosensors for the rapid diagnosis of viral infections, such as COVID-19, middle east respiratory syndrome (MERS), influenza, hepatitis, human immunodeficiency virus (HIV), and dengue, among others. The role and relevance of the nanomaterial, the type of biosensor, and the electrochemical technique adopted will be discussed. Finally, the critical issues in applying laboratory research to the analysis of real samples, future perspectives, and commercialization aspects of electrochemical biosensors for virus detection will be analyzed.

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Development of Aptamer‐Based Electrochemical Methods

Cited by 2 publications

References 31 publications

Recent Advances in Electrochemical Sensing Strategies for Food Allergen Detection

Recent Advances in Electrochemical Sensing Strategies for Food Allergen Detection

Functional Nanomaterials Enhancing Electrochemical Biosensors as Smart Tools for Detecting Infectious Viral Diseases

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