Development of self-assembled nanocarriers to enhance antitumor efficacy of docetaxel trihydrate in MDA-MB-231 cell line

Rarokar, Nilesh R.; Khedekar, Pramod B.; Bharne, Ashish P.; Umekar, Milind J.

doi:10.1016/j.ijbiomac.2018.12.130

Cited by 28 publications

(16 citation statements)

References 40 publications

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“…We used this technique to optimize the NCS formulation following the individual response properties using DOE software (Design‐Expert®, Version 10.0.4.0., Stat‐Ease Inc., Minneapolis, MN). The central composite design of experiment (CCD) is known for the optimization of several processing parameters like time, temperature, and rpm 39‐41 . The formulation variables like concentration of GMO lipid ( X 1 , %vol/vol), Pluronic® F127 surfactant ( X 2 , %wt/vol), and MLX drug ( X 3 , %wt/vol) were taken into consideration to study their influence on the final particle size.…”

Section: Methodsmentioning

confidence: 99%

Meloxicam encapsulated nanostructured colloidal self‐assembly for evaluating antitumor and anti‐inflammatory efficacy in 3D printed scaffolds

Rarokar

Ravikumar

Gurav

et al. 2020

J Biomedical Materials Res

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Nanostructured colloidal self‐assembly (NCS) is one of the most promising drug delivery carriers in cancer treatment. The present research work aimed towards synthesizing meloxicam (MLX) loaded NCS for its improved circulation half‐life and increased cellular internalization. NCS was formulated using glyceryl monoolein, Pluronic® F127, and MLX. Quality by Design experiments with a quadratic model was subjected to optimization of the formulation. The optimized NCS with an average particle size of 185.5 ± 3.02 nm showed higher MLX encapsulation (94.74 ± 3.41%) and sustained release behavior of MLX up to 24 hr. in vitro cytotoxicity of the developed NCS with MCF‐7 and MDA‐MB‐231 cell lines confirmed lower cell viability and a higher rate of cell growth inhibition. This MLX loaded NCS showed dual activity as an antitumor and anti‐inflammatory in highly invasive estrogen‐dependent MDA‐MB‐231 cells due to the high expression of cyclooxygenase‐2 (COX‐2). Besides, an activity of the MLX‐NCS was also observed in 3D printed MCF‐7 cells. This investigation shows the possible use of MLX‐NCS as an efficient cancer drug delivery system with excellent colloidal stability, sustained release of MLX, enhanced antitumor and anti‐inflammatory efficacy in 3D printed scaffolds. In contrast to toxicity study in 2D culture, the 3D constructs revealed the activity of the MLX via COX‐2 independent mechanism and demonstrated that the relationship between COX‐2 expression and antitumor activity of inhibitors is limited. In conclusion, the overall observations and results of this study strengthen the hypothesized development of NCS as a next‐generation therapeutics regimen for cancer therapy.

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Section: Methodsmentioning

confidence: 99%

Meloxicam encapsulated nanostructured colloidal self‐assembly for evaluating antitumor and anti‐inflammatory efficacy in 3D printed scaffolds

Rarokar

Ravikumar

Gurav

et al. 2020

J Biomedical Materials Res

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“…Although viral vectors have shown high transfection efficiency, several drawbacks limit their clinical application, for example, severe immune inflammatory reactions and the risk of recombination with wild-type viruses [9,10]. In contrast, non-viral vectors have an extensive delivery capacity, low immunogenicity, and structural flexibility, making them excellent alternatives to viral vectors [11][12][13]. With the rapid development of nanotechnology, many nanoparticles have been explored for potential use as gene delivery carriers [14,15].…”

Section: Introductionmentioning

confidence: 99%

Functionalized Folate-Modified Graphene Oxide/PEI siRNA Nanocomplexes for Targeted Ovarian Cancer Gene Therapy

et al. 2020

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Gene therapy is emerging as a valid method for the treatment of ovarian cancer, including small interfering RNA (siRNA). Although it is so powerful, few targeting efficient gene delivery systems seriously hindered the development of gene therapy. In this study, we synthesized a novel gene vector PEG-GO-PEI-FA by functionalized graphene oxide (GO), in which folic acid (FA) can specifically bind to the folate receptor (FR), which is overexpressed in ovarian cancer. Characterizations of the nanocomplexes were evaluated by dynamic light scattering (DLS), atomic force microscopy (AFM), and Fourier transform infrared spectroscopy (FTIR). The siRNA condensation ability and stability were assessed by agarose gel electrophoresis. Cellular uptake efficiency and lysosomal escape ability in ovarian cancer cells were investigated by confocal laser scanning microscopy. Furthermore, cellular biosafety of the system and inhibitory of the siRNA tolerability were evaluated by CCK-8 assay. The size of the PEG-GO-PEI-FA nanocomplexes was 216.1 ± 2.457 nm, exhibiting mild cytotoxicity in ovarian cancer cells. With high uptake efficiency, PEG-GO-PEI-FA can escape from the lysosome rapidly and release the gene. Moreover, PEG-GO-PEI-FA/siRNA can effectively inhibit the growth of ovarian cancer cells. By and large, the PEG-GO-PEI-FA/siRNA may offer a promising strategy for siRNA delivery in the treatment of FR-positive ovarian carcinoma or similar tumors.

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“…The hydrophobically assembled micelles usually consist of amphiphilic macromolecules that have distinct hydrophobic and hydrophilic domains, and the commonly used block segments of copolymers have been summarized [3]. Upon exposure to aqueous medium, the amphiphilic molecules are spontaneously self-assembled into supramolecular core/shell structures and water-insoluble drugs can be loaded into the hydrophobic cores [4,5].…”

Section: Introductionmentioning

confidence: 99%

Polymeric micelle as a nanocarrier for delivery of therapeutic agents: A comprehensive review

Agrawal

Tatode

Rarokar

et al. 2020

J. Drug Delivery Ther.

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For selective and effective drug delivery of therapeutic agent nanocarriers are the most effective agents. Micelles are an aggregate of surfactant molecules that dispersed in a liquid colloid. Micelles have a variety of shapes such as spheres, rods, vesicles, tubules, and lamellae. The shape and size of a micelle are a function of the molecular geometry of its surfactant molecules and solution conditions such as surfactant concentration, temperature, pH, and ionic strength. Poly Ethylene Glycol (PEG) is the most commonly used hydrophilic segment of micelles for drug delivery. Besides PEG, other polymers including poly (N-vinyl pyrrolidone) (PVP) and poly (N-isopropyl acrylamide) (pNIPAM) have also been used as hydrophilic portion of micelles. In this review we all discus about the polymeric micelles (PMs) as a nanocarriers for delivery of therapeutic agents. Keywords: Polymeric Micelles, Colloids, Nanocarriers, Drug Delivery, Poly Ethylene Glycol(PEG)

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Development of self-assembled nanocarriers to enhance antitumor efficacy of docetaxel trihydrate in MDA-MB-231 cell line

Cited by 28 publications

References 40 publications

Meloxicam encapsulated nanostructured colloidal self‐assembly for evaluating antitumor and anti‐inflammatory efficacy in 3D printed scaffolds

Meloxicam encapsulated nanostructured colloidal self‐assembly for evaluating antitumor and anti‐inflammatory efficacy in 3D printed scaffolds

Functionalized Folate-Modified Graphene Oxide/PEI siRNA Nanocomplexes for Targeted Ovarian Cancer Gene Therapy

Polymeric micelle as a nanocarrier for delivery of therapeutic agents: A comprehensive review

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