Development of semisynthetic triterpenoid saponin derivatives with immune stimulating activity

Marciani, Dante J.; Press, Jeffery B.; Reynolds, Robert C.; Pathak, Ashish; Pathak, Vibha; Gundy, L.E.; Farmer, John; Koratich, Michael; May, Richard

doi:10.1016/s0264-410x(00)00118-3

Cited by 185 publications

(121 citation statements)

References 31 publications

Supporting

Mentioning

121

Contrasting

Order By: Relevance

“…For study 2, Tat Toxoid (Neovac SA) was prepared as described (34). GPI-0100 (Hawaii Biotech) is an adjuvant derived from the soap-bark tree, Q. saponaria (Hawaii Biotech) (31)(32)(33). The adjuvant was mixed with ODN2006 (InvivoGen), a TLR agonist.…”

Section: Methodsmentioning

confidence: 99%

Balance of cellular and humoral immunity determines the level of protection by HIV vaccines in rhesus macaque models of HIV infection

Fouts

Bagley

Prado

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

119

122

View full text Add to dashboard Cite

A guiding principle for HIV vaccine design has been that cellular and humoral immunity work together to provide the strongest degree of efficacy. However, three efficacy trials of Ad5-vectored HIV vaccines showed no protection. Transmission was increased in two of the trials, suggesting that this vaccine strategy elicited CD4+ T-cell responses that provide more targets for infection, attenuating protection or increasing transmission. The degree to which this problem extends to other HIV vaccine candidates is not known. Here, we show that a gp120-CD4 chimeric subunit protein vaccine (full-length single chain) elicits heterologous protection against simian-human immunodeficiency virus (SHIV) or simian immunodeficiency virus (SIV) acquisition in three independent rhesus macaque repeated low-dose rectal challenge studies with SHIV162P3 or SIVmac251. Protection against acquisition was observed with multiple formulations and challenges. In each study, protection correlated with antibody-dependent cellular cytotoxicity specific for CD4-induced epitopes, provided that the concurrent antivaccine T-cell responses were minimal. Protection was lost in instances when T-cell responses were high or when the requisite antibody titers had declined. Our studies suggest that balance between a protective antibody response and antigen-specific T-cell activation is the critical element to vaccine-mediated protection against HIV. Achieving and sustaining such a balance, while enhancing antibody durability, is the major challenge for HIV vaccine development, regardless of the immunogen or vaccine formulation.

show abstract

Section: Methodsmentioning

confidence: 99%

Balance of cellular and humoral immunity determines the level of protection by HIV vaccines in rhesus macaque models of HIV infection

Fouts

Bagley

Prado

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

119

122

View full text Add to dashboard Cite

show abstract

“…However, ascribed to high toxicity, haemolytic effect and instability, Quillaja saponins have been restricted for use in human vaccination (Marciani et al, 2000;Liu et al, 2002).…”

Section: Quillaja Saponariamentioning

confidence: 99%

Adjuvant effects of saponins on animal immune responses

Rajput

Xiao

et al. 2007

J. Zhejiang Univ. - Sci. B

223

149

View full text Add to dashboard Cite

Abstract:Vaccines require optimal adjuvants including immunopotentiator and delivery systems to offer long term protection from infectious diseases in animals and man. Initially it was believed that adjuvants are responsible for promoting strong and sustainable antibody responses. Now it has been shown that adjuvants influence the isotype and avidity of antibody and also affect the properties of cell-mediated immunity. Mostly oil emulsions, lipopolysaccharides, polymers, saponins, liposomes, cytokines, ISCOMs (immunostimulating complexes), Freund's complete adjuvant, Freund's incomplete adjuvant, alums, bacterial toxins etc., are common adjuvants under investigation. Saponin based adjuvants have the ability to stimulate the cell mediated immune system as well as to enhance antibody production and have the advantage that only a low dose is needed for adjuvant activity. In the present study the importance of adjuvants, their role and the effect of saponin in immune system is reviewed.

show abstract

“…The capacity to elicit an effective T and B cells immunity could be shown by the stimulation of lymphocyte proliferation response (19). The splenocyte proliferation is an indicator of immunoenhancement and related to immunity improvement of T-lymphocytes or B-lymphocytes (19,20). The splenic lymphocytes proliferation was considered as an important indicator for studying immune response and evaluating cellular immune function.…”

Section: Discussionmentioning

confidence: 99%

A novel polysaccharide isolated from <i>Litchi chinensis</i> by using a simulated gastric medium and its immunomodulatory activity

Wang¹,

Hu²,

Bi³

et al. 2015

DD&T

View full text Add to dashboard Cite

Development of semisynthetic triterpenoid saponin derivatives with immune stimulating activity

Cited by 185 publications

References 31 publications

Balance of cellular and humoral immunity determines the level of protection by HIV vaccines in rhesus macaque models of HIV infection

Balance of cellular and humoral immunity determines the level of protection by HIV vaccines in rhesus macaque models of HIV infection

Adjuvant effects of saponins on animal immune responses

A novel polysaccharide isolated from <i>Litchi chinensis</i> by using a simulated gastric medium and its immunomodulatory activity

Contact Info

Product

Resources

About