Development of sensitive and selective UPLC-MS/MS method for the quantification of vitamin D<sub>2</sub> (ergocalciferol) and its demonstration in <i>Agaricus bisporus</i>

In recent years, dietary intake of vitamin D has become an issue of high concern because this bioactive molecule boosts the immune system and is presumed to provide some protection against Covid-19. Under these conditions, a search for nontraditional dietary sources has appeared as a new challenge. One of the possibilities is irradiation of champignons that contain a high amount of ergosterol, a vitamin D2 precursor. In our study, a fast and sensitive liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) method for the determination of vitamin D2 in fresh mushrooms and its metabolite 25(OH)D2 in the blood of volunteers regularly consuming UV-treated mushrooms has been introduced. For extraction of desiccated mushrooms, solid-liquid extraction n-hexane–ethyl acetate was used, and n-hexane was employed for blood plasma samples. Separation of target analytes was performed on a polymeric bonding C18 phase column. Satisfactory limits of quantification (LOQs) were reached both for the control of vitamin D2 content in mushrooms (LOQ = 10 ng/g) and for the monitoring of vitamin D2 and D3 metabolite in human blood (LOQ = 2.5 ng/mL). For accurate quantification, isotopic dilution was employed.

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Development of sensitive and selective UPLC-MS/MS method for the quantification of vitamin D₂ (ergocalciferol) and its demonstration in Agaricus bisporus

Cited by 2 publications

References 28 publications

Automated online monitoring of lactate and pyruvate in tamoxifen resistant MCF-7 cells using sequential-injection capillary electrophoresis with contactless conductivity detection (SI-CE-C⁴D) and correlation with MCT1 and MCT4 genes expression

Automated online monitoring of lactate and pyruvate in tamoxifen resistant MCF-7 cells using sequential-injection capillary electrophoresis with contactless conductivity detection (SI-CE-C⁴D) and correlation with MCT1 and MCT4 genes expression

Analysis of UV-Treated Mushrooms: Dietary Source of Vitamin D2?

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Development of sensitive and selective UPLC-MS/MS method for the quantification of vitamin D2 (ergocalciferol) and its demonstration in Agaricus bisporus

Cited by 2 publications

References 28 publications

Automated online monitoring of lactate and pyruvate in tamoxifen resistant MCF-7 cells using sequential-injection capillary electrophoresis with contactless conductivity detection (SI-CE-C4D) and correlation with MCT1 and MCT4 genes expression

Automated online monitoring of lactate and pyruvate in tamoxifen resistant MCF-7 cells using sequential-injection capillary electrophoresis with contactless conductivity detection (SI-CE-C4D) and correlation with MCT1 and MCT4 genes expression

Analysis of UV-Treated Mushrooms: Dietary Source of Vitamin D2?

Contact Info

Product

Resources

About

Development of sensitive and selective UPLC-MS/MS method for the quantification of vitamin D₂ (ergocalciferol) and its demonstration in Agaricus bisporus

Automated online monitoring of lactate and pyruvate in tamoxifen resistant MCF-7 cells using sequential-injection capillary electrophoresis with contactless conductivity detection (SI-CE-C⁴D) and correlation with MCT1 and MCT4 genes expression

Automated online monitoring of lactate and pyruvate in tamoxifen resistant MCF-7 cells using sequential-injection capillary electrophoresis with contactless conductivity detection (SI-CE-C⁴D) and correlation with MCT1 and MCT4 genes expression