Development of sex-linked PCR markers for gender identification in Actinidia

Gill, G. P.; Harvey, C. F.; Gardner, Richard C.; Fraser, Lena G.

doi:10.1007/s001220050914

Cited by 74 publications

(61 citation statements)

References 22 publications

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“…An use of molecular methods for marker-assisted selection (MAS) in large numbers of plants provides a means for rapid and reliable identification of agricultural important traits in F 1 progenies. The specific molecular markers for sex identification were derived in some dioecious plants: hemp (Sakamoto et al 1995), pistachio (Hormanza et al 1994), papaya (Parasnis et al 2000), Actinidia (Gill et al 2000), Asparagus (Reamon-Bûttner et al 1998) and Silene (Zhang et al 1998). Polley et al (1997) also developed specific PCR molecular marker associated with Y chromosome of hop.…”

mentioning

confidence: 99%

Identification of sex in F1 progenies of hop (Humulus lupulus L.) by molecular marker

Patzak¹,

Vejl²,

Skupinová³

et al. 2002

Plant Soil Environ.

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Sex identification for hop (Humulus lupulus L.), which is a dioecious plant, is very important for breeding process. The use of molecular methods for marker-assisted selection (MAS) provides rapid and reliable identification of sex in F 1 progenies. In the first, we proved the use of specific PCR molecular marker for evaluation of sex in selected plants. This marker was not amplified in 3 from 35 male plants. In the next, we successfully analysed 770 genotypes of F 1 progenies of three crossings. The amplified differences were found in 4 male plants of crossing Sm01 H28, 8 male plants of crossing Sm01 H29 and 5 female plants of crossing Sm00 H20. Statistic analysis confirmed that sex ratios of all F 1 progenies were significantly coincident. Obtained results confirmed that this specific PCR marker successfully identified sex of juvenile hop plants in F 1 progenies and effectively improved breeding process.

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mentioning

confidence: 99%

Identification of sex in F1 progenies of hop (Humulus lupulus L.) by molecular marker

Patzak¹,

Vejl²,

Skupinová³

et al. 2002

Plant Soil Environ.

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“…Various genetic markers have been developed in Actinidia using restriction fragment length polymorphisms (RFLPs) (Crowhurst et al, 1990), amplified fragment length polymorphisms (AFLPs) (Novo et al, 2010;Testolin et al, 2001; X.G. Xiao et al, 1999), random amplified polymorphic DNAs (RAPDs) (Gill et al, 1998;H.W. Huang et al, 2002a;Shirkot et al, 2002), SSRs (Fraser et al, 2004;W.G.…”

Section: Molecular Markers and Mappingmentioning

confidence: 99%

“…Much of the early molecular marker development was primarily to investigate the molecular phylogeny of Actinidia species, which in general was consistent with the traditional morphology-based classification. Studies were aimed also at sex determination, and molecular markers confirmed that the dioecious nature in Actinidia was a consequence of sex-determining genes localized on a pair of chromosomes that function like an XX/XY system (Gill et al, 1998;Harvey et al, 1997;Testolin et al, 1995). Although the genetic basis for sex determination in kiwifruit remains unknown, RAPD markers linked to this trait led to the development of sequence-characterized amplified regions (SCARs) (Gill et al, 1998).…”

Section: Molecular Markers and Mappingmentioning

confidence: 99%

“…Studies were aimed also at sex determination, and molecular markers confirmed that the dioecious nature in Actinidia was a consequence of sex-determining genes localized on a pair of chromosomes that function like an XX/XY system (Gill et al, 1998;Harvey et al, 1997;Testolin et al, 1995). Although the genetic basis for sex determination in kiwifruit remains unknown, RAPD markers linked to this trait led to the development of sequence-characterized amplified regions (SCARs) (Gill et al, 1998). These are now deployed routinely in markerassisted kiwifruit breeding, to eliminate male plants from crosses at the seedling stage, to select males when breeding for pollinizers, or to ensure a desirable male-to-female ratio of progeny are planted when characterizing families.…”

Section: Molecular Markers and Mappingmentioning

confidence: 99%

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Applications of Biotechnology in Kiwifruit (Actinidia)

Wang¹,

Gleave²

2012

Innovations in Biotechnology

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“…Esto ha generado interés por desarrollar estrategias de identificación del sexo en papaya a través de marcadores moleculares, particularmente mediante la aplicación de técnicas basadas en la reacción en cadena de la polimerasa o Pcr (por sus siglas en inglés). Varios autores han diseñado iniciadores de Pcr a partir de marcadores moleculares que permiten el diagnóstico del sexo de plantas de papaya en estados tempranos de desarrollo (gill et al 1998, Parasnis et al 2000, Deputy et al 2002, Urasaki et al 2002a, b, Chaves y Nuñez 2007. Normalmente, el diseño de los imprimadores se basa en la determinación de marcadores RAPDs (Fragmentos polimórficos de ADN Amplificados al Azar) asociados a un tipo sexual.…”

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Identificación mediante PCR del sexo de la papaya (Carica papaya L.), híbrido Pococí

et al. 2009

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resUmenIdentificación mediante PCR del sexo de la papaya (Carica papaya L.), híbrido "Pococí". el objetivo de la presente investigación fue identificar plantas hermafroditas del híbrido de papaya "Pococí". Para la identificación del sexo de las plantas del híbrido "Pococí", se utilizó el protocolo descrito por Deputy et al. (2002), con algunas modificaciones. esta metodología emplea un Pcr múltiple que permite la amplificación simultánea de dos fragmentos (1.300 y 800 pb respectivamente) para plantas hermafroditas y de un solo fragmento (1.300 pb) para plantas femeninas. el aDn se extrajo a partir de hojas de plantas de invernadero o campo con dos metodologías de extracción, cTaB y lisis alcalina (NaOH). La amplificación por PCR del ADN extraído de muestras foliares de papaya híbrido "Pococí", con ambos métodos de extracción, produjo los fragmentos del tamaño esperado. La determinación del sexo de 1.500 plántulas en almácigo mostró un 46 % de plántulas hermafroditas y un 54 % de plantas femeninas. La proporción observada de plantas femeninas: hemafroditas no varió de la esperada (1:1) según la prueba de chi-cuadrado (p= 0,4237). Las plantas hermafroditas fueron llevadas al campo y al momento de la floración se determinó su sexo. La correspondencia entre el sexado por PCR y la expresión sexual en campo fue de un 98 %.Palabras clave: Plantas hembra, hermafroditas, marcadores moleculares, extracción de aDn, genes ligados al sexo. abstractSex determination of papaya (Carica papaya l.) "Pococí" hybrid by PCR. The objective of this work was to identify papaya hermaphrodite plants of the hybrid "Pococí" at the seedling stage using the Polymerase Chain Reaction (PCR). Sex identification was achieved according to the methodology described by Deputy et al. (2002) with some modifications. This methodology employs a multiplex-PCR assay that allows simultaneous amplification of two fragments (800 bp and 1300 respectively) for hermaphrodite plants and one fragment (1300 bp) for female plants. genomic Dna was extracted from leaves and two extraction protocols were evaluated, CTAB and rapid alkaline lysis (NaOH). The amplification reaction yielded the expected size of the fragments with both methods of DNA extraction. PCR sex determination of 1500 seedlings yielded 46 % of hermaphrodite and 54 % of female plants. The female: hermaphrodite plant ratio observed did not vary from the expected (1:1) ratio according to the chi-square test (p = 0.4237). Hermaphrodite plants were planted in the field in san carlos region, costa rica, and sex was determined at flowering. The correspondence between the PCR sexed plants and sexual expression in the field was 98 %.

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Development of sex-linked PCR markers for gender identification in Actinidia

Cited by 74 publications

References 22 publications

Identification of sex in F1 progenies of hop (Humulus lupulus L.) by molecular marker

Identification of sex in F1 progenies of hop (Humulus lupulus L.) by molecular marker

Applications of Biotechnology in Kiwifruit (Actinidia)

Identificación mediante PCR del sexo de la papaya (Carica papaya L.), híbrido Pococí

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Development of sex-linked PCR markers for gender identification in Actinidia

Cited by 74 publications

References 22 publications

Identification of sex in F1 progenies of hop (Humulus lupulus L.) by molecular marker

Identification of sex in F1 progenies of hop (Humulus lupulus L.) by molecular marker

Applications of Biotechnology in Kiwifruit (Actinidia)

Identificación mediante PCR del sexo de la papaya (Carica papaya L.), híbrido Pococí

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Identificación mediante PCR del sexo de la papaya (Carica papaya L.), híbrido Pococí