Development of shoots of Hedyotis corymbosa (L) Lam in vitro culture

Abstract

Hedyotis corymbosa is one of weedy herb belonging to Rubiaceae family. It has been studied and used as a traditional medicine for the treatment of snakebite, antihepatotoxic and cancer. Notably, ursolic acid and oleanolic acid, compounds presented high biological activity in H. Corymbosa, were reported having anti-inflammatory, antibacterial, antioxidant and inhibited the growth of cancer cells. In the present study, fragments of stems containing an axillary bud are cultured on MS½ medium supplemented with 1 mg/L BA and 0.2 mg/L IAA is the best condition which gives the highest number of shoot formation. The shoots are come from the callus of cortex. In different culture conditions (increase the sucrose concentration in the medium culture, increase the light intensity, supplemented with 3 % PEG), shoots grow up strongly under 7,500 lx light intensity, especially in this culture condition the respiratory rate and the concentration of ursolic acid and oleanolic acid are the highest. The respiratory changes and the role of endogenous hormones in the shoot regeneration and the response of shoots in different culture conditions have been analyzed and discussed.

Effect of blue light on the photosynthesis and flavonoid accumulation in leaves of Hedyotis corymbosa (L.) Lam.

“…is a medicinal plant with a high content of phenolic compounds and is widely used in traditional medicine (19). In previous studies, increasing light intensity to 150 μmol.m ².s ¹ reduced ⁻ ⁻ dry biomass but increased the accumulation of secondary compounds of in vitro plants (20). In this study, we used 450 nm LED light as an artificial light source, to investigate the effect of blue light on photosynthesis and phenolic accumulation in the leaves of this herb, to improve farming methods and increase beneficial secondary compounds in this plant.…”

Colored LED Lights: Use One Color Alone or with Others for Growth in Hedyotis corymbosa In Vitro?

“…However, the raw materials collected in the wild are heterogeneous, have a high post-harvest cost, and mainly depend on environmental conditions. According to previous studies, a change in the light quality could lead to increased levels of the total phenolic and flavonoid compounds in H. corymbosa [ 27 , 28 , 29 ].…”