Development of silk fibroin/nanohydroxyapatite composite hydrogels for bone tissue engineering

Ribeiro, Marta; Moraes, Mariana Agostini de; Beppu, Marisa Masumi; García, Manuel Francisco Martínez; Fernandes, Maria Helena; Monteiro, Fernando J.; Ferraz, Maria Pia

doi:10.1016/j.eurpolymj.2015.03.056

Cited by 89 publications

(54 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hydrogels possess an interconnected network structure with high water content. The gelation of SF can be induced by sonication [74], vortex [75], heating [76], solvent treatment [77], photo-crosslinking [78], and electrogelation [79]. The rate of the gelation process is controlled by temperature, pH, fibroin concentration, and addition of other compounds.…”

Section: Structure Design Of Silk Fibroin-based Biomaterialsmentioning

confidence: 99%

“…SF/nanohydroxyapatite (nanoHA) composite hydrogel was prepared by adding ethanol as a gelling agent; this hydrogel exhibited an interconnected porous structure. The nanoHA particles were uniformly distributed in the composite hydrogel, and the compression modulus was found to increase with increasing nanoHA concentration [77]. Luo et al developed a SF/hydroxypropyl methyl cellulose (HPMC) hydrogel with remarkable mechanical performance, fabricated by simple mixing and heating (Figure 5).…”

Section: Structure Design Of Silk Fibroin-based Biomaterialsmentioning

confidence: 99%

“…In highly interconnected SF scaffolds, human dermal fibroblast cells migrated within the interconnected pores, and had been observed to reach scaffold periphery after culturing for 28 days (Figure 6B) [88]. Silk fibroin hydrogels incorporated with nanoHA showed enhanced metabolic activity and alkaline phosphatase activity of the osteoblastic cells, which could be applied in bone tissue engineering [77]. Mouse fibroblast cells were used to test the cytotoxicity of SF/HPMC hydrogels, and the results showed that the cell survival rate was over 95% (Figure 5E).…”

Section: Structure Design Of Silk Fibroin-based Biomaterialsmentioning

confidence: 99%

See 2 more Smart Citations

A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures

Wang

Wei

et al. 2017

IJMS

404

241

View full text Add to dashboard Cite

The biological performance of artificial biomaterials is closely related to their structure characteristics. Cell adhesion, migration, proliferation, and differentiation are all strongly affected by the different scale structures of biomaterials. Silk fibroin (SF), extracted mainly from silkworms, has become a popular biomaterial due to its excellent biocompatibility, exceptional mechanical properties, tunable degradation, ease of processing, and sufficient supply. As a material with excellent processability, SF can be processed into various forms with different structures, including particulate, fiber, film, and three-dimensional (3D) porous scaffolds. This review discusses and summarizes the various constructions of SF-based materials, from single structures to multi-level structures, and their applications. In combination with single structures, new techniques for creating special multi-level structures of SF-based materials, such as micropatterning and 3D-printing, are also briefly addressed.

show abstract

Section: Structure Design Of Silk Fibroin-based Biomaterialsmentioning

confidence: 99%

Section: Structure Design Of Silk Fibroin-based Biomaterialsmentioning

confidence: 99%

Section: Structure Design Of Silk Fibroin-based Biomaterialsmentioning

confidence: 99%

See 1 more Smart Citation

A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures

Wang

Wei

et al. 2017

IJMS

404

241

View full text Add to dashboard Cite

show abstract

“…Subsequently, we washed the SF extract with distilled water and dissolved it in a solution of CaCl 2 , H 2 O and CH 3 CH 2 OH 1:8:2 molar between 45 °C and 55 °C for one hour [19][20][21]. The solution underwent a dialysis process, with distilled water in cellulose acetate membranes for five days to remove the inorganic salts remaining from the previous processes.…”

Section: B Fibroin (Sf) Preparation and Characterizationmentioning

confidence: 99%

“…The solution underwent a dialysis process, with distilled water in cellulose acetate membranes for five days to remove the inorganic salts remaining from the previous processes. We calculated the concentration of the final solution from the weight of the solid after the solution dried completely [21]. We characterized the SF through a Fourier Transform (FTIR-ATR) spectroscopy in a SHIMADZU IR TRACER 100 device and through polyacrylamide gel electrophoresis to calculate the molecular weight.…”

Section: B Fibroin (Sf) Preparation and Characterizationmentioning

confidence: 99%

Degradation, water uptake, injectability and mechanical strength of injectable bone substitutes composed of silk fibroin and hydroxyapatite nanorods

Buitrago-Vásquez

Orozco

2018

Rev. Fac. Ing.

View full text Add to dashboard Cite

Injectable bone substitutes are generally composite materials capable of being extruded through a device; they consist of a solid phase inside a matrix that allows the easy movement of particles. Injectable bone substitutes have the advantage of allowing its application in situ without the need of invasive surgical techniques, guaranteeing a good recovery; for this reason, they are a promising alternative to replace conventional techniques to repair bone defects. Conventional techniques include the use of allografts and autografts, which often cause adverse reactions, and are disadvantageous for both the patient and the doctor. Furthermore, there are no reports regarding bone substitute development in Colombia, creating the necessity to research composite materials that could become injectable bone substitutes. In this study, we manufactured injectable bone substitutes with hydroxyapatite and calcium phosphate, which is most similar to bone tissue, and synthesized them in nanorods with shape and size similar to the natural hydroxyapatite found inside the body. Additionally, we used extracted silk fibroin from silkworm cocoons of Bombyx mori, a natural polymer of protein nature with high mechanical properties and excellent biocompatibility. For the materials manufactured, we evaluated degradation, in a simulated body fluid (SBF) at normal body temperature, water uptake, injectability and mechanical strength. The manufactured bone substitutes showed good degradation and water uptake properties, an approximate 97% injectability, and low mechanical resistance, indicating promising properties to be used as an injectable bone substitute.

show abstract

Biocomposite Scaffolds Derived from Renewable Resources for Bone Tissue Repair

Thangaraj

Selvamurugan

2017

Handbook of Composites From Renewable Materials

View full text Add to dashboard Cite

Development of silk fibroin/nanohydroxyapatite composite hydrogels for bone tissue engineering

Cited by 89 publications

References 54 publications

A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures

A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures

Degradation, water uptake, injectability and mechanical strength of injectable bone substitutes composed of silk fibroin and hydroxyapatite nanorods

Biocomposite Scaffolds Derived from Renewable Resources for Bone Tissue Repair

Contact Info

Product

Resources

About