Development of Simple Sequence Repeat Markers for Identification of Japanese Gentian Cultivars

Sato-Ushiku, Yuka; Shimada, Naoyuki; Saito, Misa; Yamada, Eri; Hikage, Takashi; Nakatsuka, Takashi; Nishihara, Masahiro

doi:10.2503/jjshs1.80.475

Cited by 11 publications

(11 citation statements)

References 46 publications

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“…Furthermore, DH plants have many advantages for application in basic genetic research, specially genetic mapping and mutation studies (Thomas et al 2003;Forster et al 2007;Dunwel 2010;Germanà 2011a;Chen et al 2011). In gentians, genetic research has started to exploit DH plants in molecular studies, and isolation of SSR markers and construction of a genetic map (Hikage et al 2007Sato-Ushiku et al 2011).…”

Section: Discussionmentioning

confidence: 98%

Haploid and Doubled Haploid Plant Production in Gentian (Gentiana spp.)

Doi

Takahata

2015

The Gentianaceae - Volume 2: Biotechnology and Applications

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Successful production of haploid and doubled haploid (DH) plants from male and female gametophytic cells by in vitro culture has been established in some gentians (Gentiana triflora, G. scabra, and their hybrid), which are used as ornamental plants. Plant regeneration is obtained by both anther culture (androgenesis) and unfertilized ovule/ovary culture (gynogenesis). Attention is given to culture techniques, factors influencing androgenesis and gynogenesis, the ploidy of regenerants, and utilization of the molecular genetic marker for identification of DH. Although plant regeneration is obtained by both anther culture and unfertilized ovule/ovary culture, the latter has more advantages than the former. In gentian, the establishment of the haploid and DH method has application to F1 breeding and also to basic genetic studies.

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Section: Discussionmentioning

confidence: 98%

Haploid and Doubled Haploid Plant Production in Gentian (Gentiana spp.)

Doi

Takahata

2015

The Gentianaceae - Volume 2: Biotechnology and Applications

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“…SSR markers were developed as described by Sato-Ushiku et al [18]. Genomic DNA was isolated from either G. triflora Aki6PS or G. scabra SP6A1, and then digested by either Alu I, Hae III, Mse I, or Rsa I.…”

Section: Methodsmentioning

confidence: 99%

“…For example, we developed genetic markers that discriminate among blue, pink, and white flower color [15,16]. To protect breeders’ rights, markers based on fragment length polymorphisms [sequence characterized amplified region (SCAR) and SSR markers] were developed to identify Japanese gentian cultivars [17,18]. The haplotypes of W14/15 alleles, which encode an esterase involved in cold tolerance of winter buds, provided data that clarified phylogenetic relationships in the genus Gentiana , and allowed analysis of the pedigree and breeding history of cultivars derived from those Gentiana spp.…”

Section: Introductionmentioning

confidence: 99%

Construction of the first genetic linkage map of Japanese gentian (Gentianaceae)

et al. 2012

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BackgroundJapanese gentians (Gentiana triflora and Gentiana scabra) are amongst the most popular floricultural plants in Japan. However, genomic resources for Japanese gentians have not yet been developed, mainly because of the heterozygous genome structure conserved by outcrossing, the long juvenile period, and limited knowledge about the inheritance of important traits. In this study, we developed a genetic linkage map to improve breeding programs of Japanese gentians.ResultsEnriched simple sequence repeat (SSR) libraries from a G. triflora double haploid line yielded almost 20,000 clones using 454 pyrosequencing technology, 6.7% of which could be used to design SSR markers. To increase the number of molecular markers, we identified three putative long terminal repeat (LTR) sequences using the recently developed inter-primer binding site (iPBS) method. We also developed retrotransposon microsatellite amplified polymorphism (REMAP) markers combining retrotransposon and inter-simple sequence repeat (ISSR) markers. In addition to SSR and REMAP markers, modified amplified fragment length polymorphism (AFLP) and random amplification polymorphic DNA (RAPD) markers were developed. Using 93 BC1 progeny from G. scabra backcrossed with a G. triflora double haploid line, 19 linkage groups were constructed with a total of 263 markers (97 SSR, 97 AFLP, 39 RAPD, and 30 REMAP markers). One phenotypic trait (stem color) and 10 functional markers related to genes controlling flower color, flowering time and cold tolerance were assigned to the linkage map, confirming its utility.ConclusionsThis is the first reported genetic linkage map for Japanese gentians and for any species belonging to the family Gentianaceae. As demonstrated by mapping of functional markers and the stem color trait, our results will help to explain the genetic basis of agronomic important traits, and will be useful for marker-assisted selection in gentian breeding programs. Our map will also be an important resource for further genetic analyses such as mapping of quantitative trait loci and map-based cloning of genes in this species.

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“…Since codominant microsatellite markers have been developed for only a limited number of gentian species (Li et al 2007;Sato-Ushiku et al 2011), Wang et al (2011 applied dominant but quick and universal RAPD markers for the identification of S. mussotii (+) B. scorzonerifolium somatic hybrids. Besides fragments specific for both parents, fragments not present in either of the parents were found in all the clones tested, indicating putative advanced genome recombination of parental species.…”

Section: Identification Of Somatic Hybridsmentioning

confidence: 99%

Protoplast Culture and Somatic Cell Hybridization of Gentians

Tomiczak

Mikuła

Rybczyński

2015

The Gentianaceae - Volume 2: Biotechnology and Applications

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During the last three decades, less than fifteen papers have described the results of scientific investigations in the field of gentian protoplast technology and somatic hybridization. Despite rather limited research already done on this subject, several important goals have been achieved. Protoplast-to-plant systems have been developed either for leading ornamental species or for specific medicinal plants. Two major protoplast sources were evaluated in gentians, namely differentiated leaf mesophyll cells and undifferentiated callus/cell suspensions. Plant regeneration proceeded by the two different pathways of shoot organogenesis or somatic embryogenesis. Some examples of somaclonal variation at the ploidy level were demonstrated within the pool of protoplast-derived regenerants. Totipotency exhibited by gentian protoplasts was exploited to create three different somatic hybrid combinations: intergeneric Swertia mussotii (+) Bupleurum scorzonerifolium, and interspecific Gentiana kurroo (+) G. cruciata and G. cruciata (+) G. tibetica. IntroductionPlant cell totipotency as postulated by Schleiden and Schwann in the middle of the nineteenth century is the basis of modern plant biotechnology (Vasil 2008). Based on the ability of a living single plant cell to dedifferentiate and to convert into other cell types, it is possible to obtain a completely new plant from a cell and even from its protoplast. Consequently, plant regeneration from single protoplasts underlies the genetic manipulation technologies of somatic hybridization and direct genetic transformation by DNA uptake. However, the prerequisite for practical application of these technologies is the development of efficient and reproducible protoplast-to-plant systems for the species of interest.

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Development of Simple Sequence Repeat Markers for Identification of Japanese Gentian Cultivars

Cited by 11 publications

References 46 publications

Haploid and Doubled Haploid Plant Production in Gentian (Gentiana spp.)

Haploid and Doubled Haploid Plant Production in Gentian (Gentiana spp.)

Construction of the first genetic linkage map of Japanese gentian (Gentianaceae)

Protoplast Culture and Somatic Cell Hybridization of Gentians

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