Development of simple sequence repeat markers based on whole-genome sequencing to reveal the genetic diversity of Glomerella cingulata in China

Liu, Zhaotao; Lian, Sen; Li, Baohua; Dong, Xiangli; CaiXian, Wang; Cho, Won Kyong; Liang, Wenxing

doi:10.1007/s10327-019-00886-2

Cited by 5 publications

(4 citation statements)

References 55 publications

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“…For decades, molecular markers have helped assess genetic variations within and among fungal populations ( 52 – 54 ). For Alternaria spp., several types of molecular markers have been developed to analyze a diversity of taxonomic, ecological, and epidemiological questions ( 55 – 57 ).…”

Section: Discussionmentioning

confidence: 99%

Genetic Structure and Triazole Antifungal Susceptibilities of Alternaria alternata from Greenhouses in Kunming, China

Yang

Cui

et al. 2022

Microbiol Spectr

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Section: Discussionmentioning

confidence: 99%

Genetic Structure and Triazole Antifungal Susceptibilities of Alternaria alternata from Greenhouses in Kunming, China

Yang

Cui

et al. 2022

Microbiol Spectr

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“…Five genes were included, such as the ribosomal internal transcribed spacer ( ITS ), actin ( ACT ), glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ), chitin synthase ( CHS-1 ), and β-tubulin 2 ( TUB2 ). The primer pairs for amplifying target genes were ITS1F/ITS4, ACT512F/ACT783R, GDF1/ GDR1, CHS-1-79F/CHS-1-354R, and Bt2a/Bt2b, which were adopted from previous reports [ 39 ]. Multiple sequence alignments of each gene were made with the Lasergene Suite 7.1.0 software package (DNASTAR Inc. Madison, WI) [ 40 ], and manually adjusted where necessary.…”

Section: Methodsmentioning

confidence: 99%

Comparative transcriptome analysis reveals significant differences in gene expression between pathogens of apple Glomerella leaf spot and apple bitter rot

et al. 2022

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Background Apple Glomerella leaf spot (GLS) and apple bitter rot (ABR) are two devastating foliar and fruit diseases on apples. The different symptoms of GLS and ABR could be related to different transcriptome patterns. Thus, the objectives of this study were to compare the transcriptome profiles of Colletotrichum gloeosporioides species complex isolates GC20190701, FL180903, and FL180906, the pathogen of GLS and ABR, and to evaluate the involvement of the genes on pathogenicity. Results A relatively large difference was discovered between the GLS-isolate GC20190701 and ABR-isolates FL180903, FL180906, and quite many differential expression genes associated with pathogenicity were revealed. The DEGs between the GLS- and ABR-isolate were significantly enriched in GO terms of secondary metabolites, however, the categories of degradation of various cell wall components did not. Many genes associated with secondary metabolism were revealed. A total of 17 Cytochrome P450s (CYP), 11 of which were up-regulated while six were down-regulated, and five up-regulated methyltransferase genes were discovered. The genes associated with the secretion of extracellular enzymes and melanin accumulation were up-regulated. Four genes associated with the degradation of the host cell wall, three genes involved in the degradation of cellulose, and one gene involved in the degradation of xylan were revealed and all up-regulated. In addition, genes involved in melanin syntheses, such as tyrosinase and glucosyltransferase, were highly up-regulated. Conclusions The penetration ability, pathogenicity of GLS-isolate was greater than that of ABR-isolate, which might indicate that GLS-isolate originated from ABR-isolates by mutation. These results contributed to highlighting the importance to investigate such DEGs between GLS- and ABR-isolate in depth.

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“…As a phytopathogenic fungus in agriculture, Glomerella cingulata can cause the devastating anthracnose disease of strawberry, 1 and it can also cause leaf spots on apple trees. 2 Moreover, this fungus has resulted in anthracnose symptoms (dark brown to black spots) on Eucaly trees in Korea. 3 It has also been proven to be one of the key pathogens of Liriope platyphylla with spots and blighted areas on leaves.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Pathogenic microbes can infect agricultural and aquacultural plants and animals and cause a series of diseases. As a phytopathogenic fungus in agriculture, Glomerella cingulata can cause the devastating anthracnose disease of strawberry, and it can also cause leaf spots on apple trees . Moreover, this fungus has resulted in anthracnose symptoms (dark brown to black spots) on Eucaly trees in Korea .…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Terpenoids and Polyketides from the Algicolous Fungus Byssochlamys spectabilis RR-dl-2-13

Liu

Song

Yin

et al. 2022

J. Agric. Food Chem.

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Four new carotane sesquiterpenoids, byssocarotins A−D (1−4), two new nor-sesquiterpenoids, byssofarnesin (5) and byssosesquicarin (6), and three new polyketides, byssoketides A and B (7 and 8) and (8R)-paecilocin A (9a), were obtained from a macroalga-associated strain (RR-dl-2-13) of the fungus Byssochlamys spectabilis. These isolates were identified by a combination of spectroscopic methods, including mass spectroscopy, nuclear magnetic resonance, electronic circular dichroism, and X-ray diffraction. Compounds 1−4 greatly contribute to the diversity of rarely occurring 2,15-epoxycarotane sesquiterpenoids, while 5 and 6 are degradation products of farnesane and sesquicarane precursors, respectively. Compound 7 is a structurally unique furan fatty acid derivative that possesses an aldehyde group and a large conjugated unit, and 8 features a hemiketal group. During antimicrobial assays, 8 showed antagonism against the phytopathogenic fungi Glomerella cingulata, Fusarium oxysporum f. sp. cucumerium, and F. oxysporum f. sp. cubense and the marine-derived bacteria Vibrio parahaemolyticus and V. harveyi with MIC values of 13 to 50 μg/mL.

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Development of simple sequence repeat markers based on whole-genome sequencing to reveal the genetic diversity of Glomerella cingulata in China

Cited by 5 publications

References 55 publications

Genetic Structure and Triazole Antifungal Susceptibilities of Alternaria alternata from Greenhouses in Kunming, China

Genetic Structure and Triazole Antifungal Susceptibilities of Alternaria alternata from Greenhouses in Kunming, China

Comparative transcriptome analysis reveals significant differences in gene expression between pathogens of apple Glomerella leaf spot and apple bitter rot

Antimicrobial Terpenoids and Polyketides from the Algicolous Fungus Byssochlamys spectabilis RR-dl-2-13

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