Development of sorghum mutants with improved in vitro protein digestibility by CRISPR/Cas9 editing of kafirin genes

Elkonin, L. A.; Геращенков, Г. А.; Borisenko, Natalie V.; Kenzhegulov, Odyssey A.; Sarsenova, S. Kh.; Rozhnova, Natalya A.; Panin, Valery M.

doi:10.1016/j.cj.2023.02.005

Cited by 16 publications

(3 citation statements)

References 40 publications

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“…Development of grain sorghum lines with improved digestibility of kafirins similar to that of other cereals will enhance the nutritional value of sorghum grain. Recently, Elkonin et al ( 44 ) obtained grain sorghum mutants with improved kafirin digestibility using genome editing to enhance the nutritional value of sorghum grain.…”

Section: Discussionmentioning

confidence: 99%

Exploiting Indian landraces to develop biofortified grain sorghum with high protein and minerals

Nagesh Kumar,

Ramya,

Maheshwaramma

et al. 2023

Front. Nutr.

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Sorghum (Sorghum bicolor L. Moench) is the staple cereal and is the primary source of protein for millions of people in Asia and sub-Saharan Africa. Sorghum grain value has been increasing in tropical countries including India owing to its gluten-free nature, anti-oxidant properties and low glycemic index. However, the nutrient composition of modern cultivars is declining thus necessitating genetic biofortification of sorghum to combat malnutrition and improve nutritional balance in the human diet. Keeping this in view, efforts were made to utilize valuable alleles, associated with nutrient composition, that might have been left behind in the varietal development in sorghum. The study aimed to determine the genetic improvement for nine nutritional and quality parameters (crude protein, in vitro protein digestibility (IVPD), total iron (Fe), total zinc (Zn), bioavailable Fe (%), bioavailable Zn (%), total phenolics, tannins and antioxidant activity) in the grains of 19 sorghum genotypes (high yield, drought and grain mold tolerant) developed from 11 superior India’s landraces. After selection and advancement made from 2017 to 2022 through single seed descent method, the improvement in the nine nutritional and quality parameters was assessed. Significant variation was observed for all the nine parameters among the landraces and the genotypes. Sorghum genotypes PYPS 2 and PYPS 13 recorded the highest crude protein (13.21 and 12.80% respectively) and IVPD (18.68 and 19.56% respectively). Majority of the sorghum genotypes recorded high Fe (14.21–28.41 mg/100 g) and Zn (4.81–8.16 mg/100 g). High phenolics and antioxidant activity were recorded in sorghum genotypes PYPS 18 (85.65 mg/g gallic acid equivalents) and PYPS 19 (89.78%) respectively. Selections through SSD method revealed highest improvement in genotype PYPS 10 for crude protein (32.25%), total phenolics (18.48%) and antioxidant activity (15.43%). High improvements in genotypes PYPS 12 (23.50%), PYPS 3 (26.79%), PYPS 15 (21.18%) were recorded for total Fe, available Fe and high tannins, respectively. The study demonstrated that landraces could be effectively utilized as a potential, low-cost and eco-friendly approach in sorghum genetic biofortification to improved sorghum productivity and nutritional supply in semi-arid tropics.

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Section: Discussionmentioning

confidence: 99%

Exploiting Indian landraces to develop biofortified grain sorghum with high protein and minerals

Nagesh Kumar,

Ramya,

Maheshwaramma

et al. 2023

Front. Nutr.

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“…The knockout of the SEMIDWARF-1 gene in tef through the CRISPR/Cas9 system enabled the development of lodging-resistant varieties (dwarf plants) of tef [138]. Induced site-directed mutations in the kafirin genes of sorghum by CRISPR/Cas9 improved protein digestibility and vitreous endosperm [139,140]. Herbicide-tolerant foxtail millet was developed by targeting two genes (acetolactate synthase (SiALS) and acetylcoenzyme A carboxylase (SiACC)) through CRISPR base editors (cytosine and adenosine base editors) [141].…”

Section: Identification and Characterization Of Candidate Genes From ...mentioning

confidence: 99%

Role of Genome Sequences of Major and Minor Millets in Strengthening Food and Nutritional Security for Future Generations

Maharajan,

Krishna,

Krishnakumar

et al. 2024

Agriculture

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Millets are small-seeded cereals belonging to the family Poaceae. They are considered to be climate-resilient and future nutritional food cereals for humans. Millets are resistant to biotic and abiotic stressors compared to other major cereals and thrive in low-quality soils with little maintenance and less rainfall. The importance of millets is still not well known to many people due to the lack of popularity and cultivation in semi-arid tropics of Asia and Africa. The United Nations has declared 2023 as the International Year of Millets (IYM 2023) to promote millet cultivation and popularize their health benefits globally. A few years ago, the application of molecular biology was in its infancy in millets due to the unavailability of genome sequences. Genome sequences are available for most of the millets on NCBI and Phytozome databases. In this review, we discuss the details of genome sequences for millets, candidate genes identified from the native genome of millets. The current status of quantitative trait loci and genome-wide association studies in millets are also discussed. The utilization of millet genome sequences in functional genomics research and translating the information for crop improvement will help millet and non-millet cereals survive harsh environments in the future. Such efforts will help strengthen food security and reduce malnutrition worldwide in 2050.

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“…Although numerous studies have been conducted to find out the Cd associated genes but unfortunately, till now only a few genes have been found that can take part in Cd metabolism. Such as, null mutants of low cadmium (oslcd) gene accumulated less Cd in shoots under the influence of Cd-contaminated soil (Elkonin et al 2023). Similarly, the knocked-out mutants of mir535 of brown rice accumulated 35% less Cd compared to the control under controlled stress of 2 µmol/L Cd (Yue et al 2023).…”

Section: Heavy Metalmentioning

confidence: 99%

CRISPR-Cas System, a Possible “Savior” of Rice Threatened by Climate Change: An Updated Review

Shaheen,

Ahmad,

Alghamdi

et al. 2023

Rice

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Climate change has significantly affected agriculture production, particularly the rice crop that is consumed by almost half of the world’s population and contributes significantly to global food security. Rice is vulnerable to several abiotic and biotic stresses such as drought, heat, salinity, heavy metals, rice blast, and bacterial blight that cause huge yield losses in rice, thus threatening food security worldwide. In this regard, several plant breeding and biotechnological techniques have been used to raise such rice varieties that could tackle climate changes. Nowadays, gene editing (GE) technology has revolutionized crop improvement. Among GE technology, CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein) system has emerged as one of the most convenient, robust, cost-effective, and less labor-intensive system due to which it has got more popularity among plant researchers, especially rice breeders and geneticists. Since 2013 (the year of first application of CRISPR/Cas-based GE system in rice), several trait-specific climate-resilient rice lines have been developed using CRISPR/Cas-based GE tools. Earlier, several reports have been published confirming the successful application of GE tools for rice improvement. However, this review particularly aims to provide an updated and well-synthesized brief discussion based on the recent studies (from 2020 to present) on the applications of GE tools, particularly CRISPR-based systems for developing CRISPR rice to tackle the current alarming situation of climate change, worldwide. Moreover, potential limitations and technical bottlenecks in the development of CRISPR rice, and prospects are also discussed.

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Development of sorghum mutants with improved in vitro protein digestibility by CRISPR/Cas9 editing of kafirin genes

Cited by 16 publications

References 40 publications

Exploiting Indian landraces to develop biofortified grain sorghum with high protein and minerals

Exploiting Indian landraces to develop biofortified grain sorghum with high protein and minerals

Role of Genome Sequences of Major and Minor Millets in Strengthening Food and Nutritional Security for Future Generations

CRISPR-Cas System, a Possible “Savior” of Rice Threatened by Climate Change: An Updated Review

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