Development of Specific Cell-dependent Antibody During Growth of a Syngeneic Rat Sarcoma

Basham, Connie; Currie, G. A.

doi:10.1038/bjc.1974.58

Cited by 23 publications

(11 citation statements)

References 15 publications

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“…Thus tumour immunogenicity could represent an imporant determinant of the degree of K-cell activation in tumourbearing mice. In view of the available evidence that ADCC may be one mechanism in the control of tumour growth (Pollack, 1973;Ortiz de Landazuri et al, 1974;Basham and Currie, 1974;Hersey, 1937;Zighelboim et al, 1974) and of the finding reported here, that mice bearing an immunogenic nonmetastasizing MSV-induced sarcoma show higher levels of K-cell activity than animals inoculated with metastasizing MSV-induced neoplasms, it is tempting to speculate that the degree of K-cell activation may play a role in determining the biological behaviour of experimental tumours.…”

mentioning

confidence: 77%

“…Serum collected from rodents with chemical or virus-induced tumours can render normal. lymphoid cells cytotoxic for tumour target cells or increase the cytotoxicity of immune lymphocytes (Pollack, 1973;Ortiz de Landazuri, Kedar and Fahey, 1974;Basham and Currie, 1974). Moreover, passive transfer of cell-dependent antibodies results in significant antitumoral effects in vivo, thus suggesting that ADCC could represent an important antitumoral effector mechanism (Hersey, 1973;Zighelboim, Bonavida and Fahey, 1974).…”

mentioning

confidence: 99%

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Activation of K cells in mice with transplanted tumours differing in immunogenicity and metastasizing capacity

Mantovani¹,

Polentarutti²,

Alessandri³

et al. 1977

Br J Cancer

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Summary.-The effector arm of antibody-dependent cellular cytotoxicity (ADCC) was evaluated using 5lCr-labelled chicken erythrocytes as targets in BALB/c mice transplanted with the Moloney sarcoma virus-induced tumours T-MSV and MS2, and in C57BL/6 mice transplanted with the chemically induced FS6 sarcoma, Lewis lung carcinoma and B16 melanoma. Tumour-bearing animals showed higher levels of ADCC than normal mice, a stimulation confirmed in MS2-bearing mice, using SL2 lymphoma cells as targets in a cytostasis assay.ADCC effector-cell capacity was higher in animals transplanted with the immunogenic, spontaneously regressing T-MSV than in mice bearing the poorly immunogenic metastasizing MS2 sarcoma. The increased ADCC activity detectable in the spleen of tumour-bearing hosts was not abolished by removal of phagocytic -adherent cells.

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mentioning

confidence: 77%

mentioning

confidence: 99%

Activation of K cells in mice with transplanted tumours differing in immunogenicity and metastasizing capacity

Mantovani¹,

Polentarutti²,

Alessandri³

et al. 1977

Br J Cancer

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“…Again, no abrogation of immune lymphocyte cytotoxicity was observed ( Table IV, Experiments of concomitant tumour immunity (Bashford et al, 1908) were performed as other studies reported that the presence of concomitant tumour immunity correlated with the absence of blocking factors (Deekers et al, 1973;Sjogren and Bansal, 1971). 104 H50 cells were injected intradermally in the intrascapular area of 32 hamsters and on the following 4 consecutive weeks groups of Table V indicate that hamsters bearing a primary H50 tumour (palpable-2-0 cm size) showed a heightened resistance and not enhanced growth of a secondary challenge compared with normal controls.…”

Section: W1inn Neutralization Assaymentioning

confidence: 97%

“…Such sera demonstrated characteristics similar to sera taken from animals rendered immune to tumour transplantation by various procedures. These immune sera augmented the cytotoxic reactivity of tumour immune lymphocytes in vitro (potentiation) (Lamon et al, 1974) and also induced normal lymphocytes to specifically kill tumour cells (arming) (Basham and Currie, 1974).…”

Section: Mixed Haemadsorption Testmentioning

confidence: 99%

“…The phenomenon was originally called lymphocyte dependent antibody but is now referred to as cell dependent antibody (Basham and Currie, 1974) or antibody dependent cellular cytotoxicity (DeLandazuri, Kedar and Fahey, 1974) as the effector cell(s) has not been exclusively identified as a lymphocyte. Functionally immune serum can "arm" non-sensitized effector cells (Pollack et al, 1972) or "potentiate" the cytotoxic effect of immune effector cells (Hellstrom et al, 1973).…”

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Significance of arming, potentiating and blocking factors as correlates of tumour-host interaction in the hamster SV40 system

Goldrosen¹,

Dent²

1975

Br J Cancer

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Summary.-The study of blocking factors requires a reliable in vitro assay of cell mediated immunity that parallels the in vivo response. By microcytotoxicity testing, progressor and immune peripheral blood lymphocytes caused significant target cell reduction. The cytotoxicity was specific as no cytotoxic effect was detected against unrelated normal as well as malignant target cell lines. No anti-tumour effect was noted when progressor peripheral blood lymphocytes were evaluated in the Winn assay. In marked contrast, immune peripheral blood lymphocytes were capable of preventing tumour growth in the Winn assay. Furthermore, hamsters repeatedly immunized with irradiated SV40 tumour cells could resist a live cell challenge. Thus immune peripheral blood lymphocytes were chosen as the effector population to evaluate the abrogation ability of serum in a microcytotoxicity assay.Serum from hamsters with progressively growing SV40 tumours did not block, neutralize or potentiate in vitro lymphocytotoxicity. However, serum taken from hamsters before the appearance of a palpable tumour, potentiated in vitro lymphocytoxicity. Serum taken from hamsters hyperimmunized with irradiated SV40 tumour cells, live SV40 virus or following complete excision of a growing SV40 tumour armed normal effector cells and potentiated immune effector cells resulting in enhanced target cell destruction. Serum alone was not cytotoxic, suggesting a serum dependent cellular cytotoxicity effect.Serum from hamsters in which tumours recurred after excision was capable of abrogating in vitro lymphocytoxicity. However, no antibody activity was detectable to cell surface antigens by the mixed haemadsorption test in the sera that demonstrated blocking or potentiating activity. Progressor serum was unable to enhance the growth of SV40 tumour cells in vivo.The potentiating and arming activity of serum in this study directly parallels the cytostatic antibody activity described by Coggin et al. in the hamster SV40 system. Both cytostatic antibody activity and enhancement of in vitro lymphocytotoxicity activity correlates with in vivo tumour resistance. These results suggest that progressive primary tumour growth in this system may correlate with a lack of serum dependent cellular cytotoxicity rather than blocking factors, while the latter may be associated with recurrent and/or metastatic disease.

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Serum factors modifying cell mediated immunity to rat hepatoma D23 correlated with tumour growth

Bowen

Robins

Baldwin

1975

Intl Journal of Cancer

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Sera from rats bearing a progressively growing transplanted aminoazo-dye-induced hepatoma (hepatoma D23) have been examined for the presence of hepatoma D23-specfic antigen, antibody and immune complexes throughout the course of tumour growth. The levels of these factors have been correlated with the in vitro blocking and inhibition of cytotoxic lymph-node cells from immunized animals for cultured tumour cells. Sera from animals bearing small tumours (7-14 days after tumour implantation contain free tumour-specific antigen whilst immune complexes could not be detected. Although these sera were neither blocking nor inhibitory under the normal conditions of the test, when concentrated two and fourfold, inhibition but not blocking of lymph-node cell cytotoxicity could be detected. In comparison sera from animals bearing large tumours (24-28 days) blocked but did no inhibit in vitro lymph-node cell cytotoxicity and this correlates with the presence of tumour-specific immune complexes in antibody excess. Animals with intermediate-sized tumours had high levels of both blocking and inbibitory activity in the serum, these effects becoming apparent when neither free antibody nor free antigen could be detected. The relevance of these findings to the mechanism by which a growing tumour may escape specific cellular immune destruction is discussed.

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Development of Specific Cell-dependent Antibody During Growth of a Syngeneic Rat Sarcoma

Cited by 23 publications

References 15 publications

Activation of K cells in mice with transplanted tumours differing in immunogenicity and metastasizing capacity

Activation of K cells in mice with transplanted tumours differing in immunogenicity and metastasizing capacity

Significance of arming, potentiating and blocking factors as correlates of tumour-host interaction in the hamster SV40 system

Serum factors modifying cell mediated immunity to rat hepatoma D23 correlated with tumour growth

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