Development of specific immobilization method on gold surface and its application for determining cardiac risk

Kim, Gi Wook; Zheng, Shaoyong; Kim, Myung Sun; Cheon, Seon Ah; Ko, Seong Young; Park, Tae Jung

doi:10.1007/s13206-014-8408-4

Cited by 4 publications

(3 citation statements)

References 28 publications

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“…The decrease in the peak current (Δ I p , defined as the difference from the SWV with [CRP] = 0) varies linearly with the logarithm of the CRP concentration (Figure A inset). The obtained concentration range (1.0–100 μg mL –1 ) with a detection limit (LOD) (LOD = 3σ/ S , where σ is the standard deviation of the blank (ITO/TESPSA/P3 or mAb without CRP), and S is the slope of the linear region of the calibration curve (Figure A,C inset) of 0.34 μg mL –1 for ITO/TESPSA/P3 is lower than CRP-binding phage-based electrodes and similar to those obtained for electrodes modified with anti-CRP antibodies, ,, and aptamers that have been presented by others (Table S2). However, the antibody electrode presented in this article has a significantly higher LOD (3.6 μg mL –1 ) and linear response range from 5 to 50 μg mL –1 (Figure C inset), which shows that the ITO/TESPSA/mAb electrode is far from the optimized design.…”

Section: Resultssupporting

confidence: 71%

“…A solution might be characterization of the molecular interactions of CRP with receptors using various physicochemical techniques. Some examples are 2D DNA origami nanostructures for quantitative single-molecule biosensing, SERS, fluorescence microscopy, atomic force microscopy, biolayer interferometry, or SPR ,, and electrochemical methods. ,,– Not all of these are easy to perform, but they can give deeper analysis and insights into understanding protein–peptide interactions.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of Peptides for Molecular Recognition of C-Reactive Protein–Theoretical and Experimental Studies

Szot-Karpińska,

Kudła,

Orzeł

et al. 2023

Anal. Chem.

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We investigate the interactions between C-reactive protein (CRP) and new CRP-binding peptide materials using experimental (biological and physicochemical) methods with the support of theoretical simulations (computational modeling analysis). Three specific CRP-binding peptides (P2, P3, and P9) derived from an M13 bacteriophage have been identified using phage-display technology. The binding efficiency of the peptides exposed on phages toward the CRP protein was demonstrated via biological methods. Fibers of the selected phages/peptides interact differently due to different compositions of amino acid sequences on the exposed peptides, which was confirmed by transmission electron microscopy. Numerical and experimental studies consistently showed that the P3 peptide is the best CRP binder. A combination of theoretical and experimental methods demonstrates that identifying the best binder can be performed simply, cheaply, and fast. Such an approach has not been reported previously for peptide screening and demonstrates a new trend in science where calculations can replace or support laborious experimental techniques. Finally, the best CRP binderthe P3 peptidewas used for CRP recognition on silicate-modified indium tin oxide-coated glass electrodes. The obtained electrodes exhibit a wide range of operation (1.0–100 μg mL–1) with a detection limit (LOD = 3σ/S) of 0.34 μg mL–1. Moreover, the dissociation constant K d of 4.2 ± 0.144 μg mL–1 (35 ± 1.2 nM) was evaluated from the change in the current. The selectivity of the obtained electrode was demonstrated in the presence of three interfering proteins. These results prove that the presented P3 peptide is a potential candidate as a receptor for CRP, which can replace specific antibodies.

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Section: Resultssupporting

confidence: 71%

Section: Introductionmentioning

confidence: 99%

Investigation of Peptides for Molecular Recognition of C-Reactive Protein–Theoretical and Experimental Studies

Szot-Karpińska,

Kudła,

Orzeł

et al. 2023

Anal. Chem.

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“…Therefore, how to improve the thermal conductivity of polymers has become a focus for researchers and industry. Although various attempts have been made to design polymer chains to increase thermal conductivity [ 10 , 11 ], higher cost and complex processes are the two main obstacles hampering its practical application. In addition to designing the structure and morphology of the polymer itself, another effective method to improve the thermal conductivity of the polymer is to impregnate the polymer with thermally conductive fillers.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Monotrimethoxylsilylethyl-Terminated Polysiloxane Fluids and Their Application in Thermal Interface Materials

Liu

Wang

et al. 2023

Polymers

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In this study, α-Trimethylsilylmethyl-ω-dimethylsilyl-terminated polydimethylsiloxane, polydiethylsiloxane and poly[2,2,2-trifluoropropyl(methyl)siloxane] are synthesized using an anion catalyzed nonequilibrium polymerization reaction with trimethylsilylmethyl lithium as the initiator; hexamethylcyclotrisiloxane, hexaethylcyclotrisiloxane or 1,3,5-trimethyl-1,3,5-trifluoropropylcyclotrisiloxane as the monomer; and dimethylchlorosilane as an end-capping agent. Three kinds of α-trimethylsilylmethyl-ω-trimethoxylsilylethyl-terminated polysiloxanes are further prepared by hydrosilylation reaction of α-trimethylsilylmethyl-ω-dimethylsilyl-terminated polysiloxanes with vinyltrimethoxysilane using Karstedt’s catalyst. These α-trimethylsilylmethyl-ω-trimethoxylsilylethyl-terminated polysiloxanes are functionalized as in situ surface treatment agents for AlN particles. The effects of the structure of these polysiloxanes on the dispersion of AlN in the polysiloxane matrix and on the heat transfer performance of silicone pastes and silicone rubbers are investigated. A possible mechanism of surface treatment of AlN fillers by these novel silicone fluids is also discussed.

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Recent advances of electrochemical and optical biosensors for detection of C-reactive protein as a major inflammatory biomarker

Sohrabi

Kordasht

Pashazadeh-Panahi

et al. 2020

Microchemical Journal

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Development of specific immobilization method on gold surface and its application for determining cardiac risk

Cited by 4 publications

References 28 publications

Investigation of Peptides for Molecular Recognition of C-Reactive Protein–Theoretical and Experimental Studies

Investigation of Peptides for Molecular Recognition of C-Reactive Protein–Theoretical and Experimental Studies

Preparation of Monotrimethoxylsilylethyl-Terminated Polysiloxane Fluids and Their Application in Thermal Interface Materials

Recent advances of electrochemical and optical biosensors for detection of C-reactive protein as a major inflammatory biomarker

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