Development of specific PCR primer sets for detecting Pantoea dispersa, a potential biocontrol agent against rice seed-borne diseases caused by Burkholderia pathogens

Kouzai, Yusuke; Akimoto-Tomiyama, Chiharu

doi:10.1007/s10327-023-01154-0

J Gen Plant Pathol

2023

DOI: 10.1007/s10327-023-01154-0

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Development of specific PCR primer sets for detecting Pantoea dispersa, a potential biocontrol agent against rice seed-borne diseases caused by Burkholderia pathogens

Yusuke Kouzai,

Chiharu Akimoto-Tomiyama

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2024

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Cited by 2 publications

References 38 publications

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Bacterial panicle blight caused by Burkholderia glumae : a major disease in rice cultivation

Azzahra,

Miranti,

Prismantoro

et al. 2024

Cogent Food & Agriculture

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Bacterial panicle blight caused by Burkholderia glumae : a major disease in rice cultivation

Azzahra,

Miranti,

Prismantoro

et al. 2024

Cogent Food & Agriculture

View full text Add to dashboard Cite

BglaTNB6, a tailocin produced by a plant-associated nonpathogenic bacterium, prevents rice seed-borne bacterial diseases

Kouzai,

Sagehashi,

Watanabe

et al. 2024

PLoS Pathog

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Rice seed-borne diseases caused by the bacterial pathogens Burkholderia glumae and B. plantarii pose a major threat to rice production worldwide. To manage these diseases in a sustainable manner, a biocontrol strategy is crucial. In this study, we showed that B. gladioli NB6 (NB6), a nonpathogenic bacterium, strongly protects rice from infection caused by the above-mentioned pathogens. NB6 was isolated from the indica rice cultivar Nona Bokra seedlings, which possesses genetic resistance to B. glumae. We discovered that cell suspensions of NB6 and its culture filtrate suppressed the disease symptoms caused by B. glumae and B. plantarii in rice seedlings, which indicated that NB6 secretes a plant-protective substance extracellularly. Through purification and mass spectrometry analysis of the culture filtrate, combined with transmission electron microscopy and mutant analysis, the substance was identified as a tailocin and named BglaTNB6. Tailocins are bacteriotoxic multiprotein structures morphologically similar to headless phage tails. BglaTNB6 exhibited antibacterial activity against several Burkholderia species, including B. glumae, B. plantarii, and B. gladioli, suggesting it can prevent pathogen infection. Interestingly, BglaTNB6 greatly contributed only to the biocontrol activity of NB6 cell suspensions against B. plantarii, and not against B. glumae. BglaTNB6 was shown to be encoded by a prophage locus lacking genes for phage head proteins, and a B. gladioli strain with the coded BglaTNB6-like locus equipped with phage head proteins failed to prevent rice seedlings from being infected with B. plantarii. These results suggested that BglaTNB6 may enhance the competitiveness of NB6 against a specific range of bacteria. Our study also highlights the potential of tailocin-producing endophytes for managing crop bacterial diseases.

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Development of specific PCR primer sets for detecting Pantoea dispersa, a potential biocontrol agent against rice seed-borne diseases caused by Burkholderia pathogens

Cited by 2 publications

References 38 publications

Bacterial panicle blight caused by Burkholderia glumae : a major disease in rice cultivation

Bacterial panicle blight caused by Burkholderia glumae : a major disease in rice cultivation

BglaTNB6, a tailocin produced by a plant-associated nonpathogenic bacterium, prevents rice seed-borne bacterial diseases

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