Development of specific PCR primers for identification and detection of Phytophthora capsici Leon

Silvar, Cristina; Duncan, James M.; Cooke, D. E. L.; Williams, N. A.; Dı́az, José; Merino, Fuencisla

doi:10.1007/s10658-004-8232-0

Cited by 66 publications

(26 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These samples were all positive for the detection of P. capsici. The symptoms presented by the plants affected by this pathogen are -similar to the symptoms of Verticillium wilt which can lead to confusion (Silvar et al 2005b). We were unable to detect Verticillium dahliae in plots B2 and B3, where disease symptoms were slight, or in B5, where disease symptoms were not observed.…”

Section: Discussionmentioning

confidence: 71%

“…We were unable to detect Verticillium dahliae in plots B2 and B3, where disease symptoms were slight, or in B5, where disease symptoms were not observed. Interestingly, P. capsici was detected in the two plants collected from greenhouse B2 (corresponding to Farm 4 in Silvar et al 2005b). Practical application of the described method seems clear, since it was possible to detect the pathogen in plants, soil and water before visible symptoms of the disease appeared.…”

Section: Discussionmentioning

confidence: 89%

See 1 more Smart Citation

Assessment of real-time PCR as a method for determining the presence of Verticillium dahliae in different Solanaceae cultivars

Gayoso

Ilárduya²,

Pomar³

et al. 2007

Eur J Plant Pathol

View full text Add to dashboard Cite

Real-time PCR was used to detect and quantify Verticillium dahliae and to assess the susceptibility of four Capsicum annuum cultivars (Luesia, Padrón, SCM331 and PI201234) and the Capsicum chinense cv. C118 to this pathogen. The symptoms which developed after infection included stunting and yellowing, and were more acute in the cv. SCM331, which also suffered defoliation in later stages of the disease and in C118, which suffered severe stunting. Quantification of the pathogen DNA in roots 23 and 34 days post-inoculation (dpi) revealed that there were significantly higher amounts of Verticillium dahliae DNA in C118 than in the other cultivars, followed by SCM331, Padrón and PI201234. The lowest amounts of fungal DNA in roots were found in Luesia. In hypocotyls, the highest amounts of fungal DNA were found in SCM331, while Luesia, Padrón and PI201234 had much lower amounts, and C118 had intermediate levels. When a compatible versus an incompatible system was studied, using the near-isogenic tomato lines LA3030 (susceptible) and LA3038 (resistant to V. dahliae), we were able to detect fungal DNA in both lines. As expected, the fungus/plant DNA ratio was lower in LA3038 than in LA3030 and it decreased with time in LA3038. The amount of Verticillium dahliae DNA in the roots of LA3030 remained constant between days 23 and 34 post-inoculation, but increased 10-fold in collars. Finally, when real-time PCR was applied as a diagnostic method to samples from pepper plants, soil and water collected from farms in northwest Spain, we were able to detect V. dahliae DNA in these samples even when symptoms of the disease were not evident.

show abstract

Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 89%

Assessment of real-time PCR as a method for determining the presence of Verticillium dahliae in different Solanaceae cultivars

Gayoso

Ilárduya²,

Pomar³

et al. 2007

Eur J Plant Pathol

View full text Add to dashboard Cite

show abstract

“…For instance, Silvar et al (2005) showed that sensitivity of detection of Phytophthora capsici could be significantly increased using nested-PCR compared to a conventional PCR assay. While in conventional PCR, the lowest amount of amplified DNA was 5 pg for the primers used, in the nested-PCR, the detection limit was 0.5 fg of template DNA.…”

Section: Discussionmentioning

confidence: 99%

The development of a PCR-based method for detecting Puccinia striiformis latent infections in wheat leaves

et al. 2007

View full text Add to dashboard Cite

Stripe rust of wheat caused by Puccinia striiformis f. sp. tritici is one of the most important diseases on wheat worldwide, especially in temperate regions with cool moist weather conditions. A rapid and reliable detection of the pathogen in latent infected wheat leaves during overwintering of the fungus in the dormant stage will contribute to determine the initial inoculum potential and thus to predict early outbreak and to improve effective management of the disease. To achieve this aim, a PCR-based method was developed for specific and sensitive detection of P. striiformis. Specific primers were designed according to a genome-specific sequence of P. striiformis. To evaluate the specificity of the primers, seven different isolates and races of P. striiformis as well as six other pathogens of wheat were tested. All isolates of P. striiformis yielded a distinct band of a fragment of 470 bp, while using DNA of the other wheat pathogens as a template no amplification product was detected. The sensitivity of the primers was tested using serial dilutions of total DNA from P. striiformis; the limit of detection was 10 pg of DNA. Using extracts from P. striiformis-infected wheat leaves, the fungus could be determined in the leaves before symptoms appeared. The stripe rust could also be detected in the dormant stage by the PCR assay in samples of wheat leaves taken during the winter season. The application of the PCR assay may be useful for rapid and reliable detection of P. striiformis in latent infected leaves of overwintering wheat plants.

show abstract

“…The biomass of P. capsici in the inoculated Arabidopsis leaf tissues was determined by qPCR during a time course (24, 36, 60, and 72 hpi) using Phytophthora-specific primers amplifying the ITS region (Silvar et al 2005). The primer pair AtRub-F4 and AtRub-R4 amplifying the gene encoding the large subunit of RuBisCo was used as the internal standard.…”

Section: P Capsici Infection Assay Of Arabidopsis Leavesmentioning

confidence: 99%

Phytophthora Suppressor of RNA Silencing 2 Is a Conserved RxLR Effector that Promotes Infection in Soybean and Arabidopsis thaliana

Xiong¹,

Ye²,

Choi³

et al. 2014

MPMI

View full text Add to dashboard Cite

The genus Phytophthora consists of notorious and emerging pathogens of economically important crops. Each Phytophthora genome encodes several hundreds of cytoplasmic effectors, which are believed to manipulate plant immune response inside the host cells. However, the majority of Phytophthora effectors remain functionally uncharacterized. We recently discovered two effectors from the soybean stem and root rot pathogen Phytophthora sojae with the activity to suppress RNA silencing in plants. These effectors are designated Phytophthora suppressor of RNA silencing (PSRs). Here, we report that the P. sojae PSR2 (PsPSR2) belongs to a conserved and widespread effector family in Phytophthora. A PsPSR2-like effector produced by P. infestans (PiPSR2) can also suppress RNA silencing in plants and promote Phytophthora infection, suggesting that the PSR2 family effectors have conserved functions in plant hosts. Using Agrobacterium rhizogenes-mediated hairy roots induction, we demonstrated that the expression of PsPSR2 rendered hypersusceptibility of soybean to P. sojae. Enhanced susceptibility was also observed in PsPSR2-expressing Arabidopsis thaliana plants during Phytophthora but not bacterial infection. These experiments provide strong evidence that PSR2 is a conserved Phytophthora effector family that performs important virulence functions specifically during Phytophthora infection of various plant hosts.

show abstract

Development of specific PCR primers for identification and detection of Phytophthora capsici Leon

Cited by 66 publications

References 30 publications

Assessment of real-time PCR as a method for determining the presence of Verticillium dahliae in different Solanaceae cultivars

Assessment of real-time PCR as a method for determining the presence of Verticillium dahliae in different Solanaceae cultivars

The development of a PCR-based method for detecting Puccinia striiformis latent infections in wheat leaves

Phytophthora Suppressor of RNA Silencing 2 Is a Conserved RxLR Effector that Promotes Infection in Soybean and Arabidopsis thaliana

Contact Info

Product

Resources

About