Development of Spectrophotometric Method for the Quantitative Determination of Betamethasone 17- Valerate in Creams

Abstract

A simple spectrophotometric technique has been developed and validated for determination of betamethasone 17-valerate in pharmaceutical formulations. The method is based on cream dissolution in absolute ethanol and assaying spectrophotometrically at 240nm. The amount of betamethasone 17-valerate in cream was determined as 101.6 ± 0.0037 % w/w. The method validation demonstrated linearity for concentration range of 0.006mg/ml to 0.0014mg/ml (R2=0.9995). The method is simple and accurate and has successfully been employed in the analysis of some of Tripharm pharmaceutical formulations.