Development of spray‐ and freeze‐dried high‐concentration sesamol emulsions and antioxidant evaluation in fibroblasts and UV‐exposed rat skin slices

Abstract

Dry sesamol emulsions were synthesized from several combinations of saccharose with hydroxypropylmethylcellulose (HPMC) or sodium caseinate (SC) using spray-drying techniques at 1201 to 1801C, or freeze-drying. On the basis of physical characteristics such as droplet size distribution, residual moisture, and microscopic structure, the best material was obtained when spray-drying was applied at either 1501 or 1801C with SC or HPMC as excipients, respectively. The extent to which the antioxidant properties of free sesamol towards a set of free radicals (galvinoxyl, diphenylpicrylhydrazyl, superoxide, and hydroxyl) were altered in the starting and reconstituted liquid emulsions submitted to normal storage or pre-exposed to a flux hydroxyl radicals was investigated. Emulsions were further evaluated for their antioxidant properties in cultured 3T3 murine fibroblasts and in an ex vivo model of ultraviolet irradiated rat skin. It was found that, in the material having the best physical properties, encapsulation was decisive in: (1) improving the overall antioxidant behavior of reconstituted versus starting liquid emulsions: (2) sparing sesamol consumption due to free radical attack; and (3) significantly protecting cells and skin against free radical-or irradiation-induced enzymatic release and/or lipid peroxidation. Demonstrating a high activity at high dilutions where interactions of excipient become negligible, the new emulsions could be of great interest in sesamol-based pharmacology or topical applications. Drug Dev Res 69: 251-266, 2008.

Formation and Characterization of Lactoferrin-Hyaluronic Acid Conjugates and Their Effects on the Storage Stability of Sesamol Emulsions

“…The extraction was repeated three times and the total sesamol content was determined in the combined n -hexane. After appropriate dilutions with n -hexane, sesamol was determined colorimetrically at 298 nm [32] on a Shimadzu UV 1240 spectrophotometer (UV-1240 Shimadzu, Tokyo, Japan). The concentration of sesamol was obtained by referring to a standard curve of sesamol prepared under the same conditions.…”

Antidiabetic, antioxidant and anti inflammatory properties of water and n-butanol soluble extracts from Saharian Anvillea radiata in high-fat-diet fed mice

“…For each extract a DPPH assay was carried out using a reported procedure (Alencar et al, 2008). Aliquots of each extract dissolved in methanol (20660 µg/mL, final concentration) were mixed with 200 µL of a fresh DPPH solution (SigmaAldrich; 0.126 mM) in methanol and the final volume was adjusted to 300 µL.…”

Improving the Antioxidant Properties of Calophyllum inophyllum Seed Oil from French Polynesia: Development and Biological Applications of Resinous Ethanol-Soluble Extracts

“…Despite sesame oil being relatively rich in antioxidants, including sesamol [ 21 , 22 ], TO was found to be more active in both DPPH and TRAP assays, suggesting that the oil contains more lipophilic antioxidants than sesame oil. Moreover, the antioxidant properties of TO may rely mainly on the resin part since the de-resinated DTO was found to be inactive.…”

“…Skin samples were shaved, rinsed first in water and then three times in 125 mM aqueous KCl, dried, cut into slices of about 15 × 10 × 0.5 mm size and kept for 1 h on agar plates at 4 • C [21]. Afterwards, skin samples were placed at the surface of a filter paper soaked with aqueous KCl to avoid any dehydration and maintained for 10 min at 37 • C. Each test extract (50 µg) was emulsified with 100 µL of olive oil and spread on the surface of the skin slice [16].…”

“…In our continuous interest in developing easy handling pharmaceutical preparations from natural sources of great economic potential [ 21 , 22 ], the purpose of the present study was (i) to prepare novel soluble TO extracts and subfractions with enhanced inhibition properties toward a host of reactive oxygen species (ROS); (ii) to screen them for their antioxidant, anti-inflammatory and antimicrobial properties using a battery of physicochemical and biochemical tests; and (iii) to further isolate and characterize in these TO fractions those compounds showing the greatest potential for biological applications.…”