Development of square-wave adsorptive stripping voltammetric method for determination of acebutolol in pharmaceutical formulations and biological fluids

Abstract

A validated simple, rapid, sensitive and specific square-wave voltammetric technique is described for the determination of acebutolol (AC) following its accumulation onto a hanging mercury drop electrode in a Britton-Robinson universal buffer of pH 7.5. The optimal procedural conditions were: accumulation potential Eacc = - 0.8 V versus Ag/AgCl/KCl, accumulation duration tacc = 30 s, pulse-amplitude = 70 mV, scan rate = 100 mV/s, frequency = 30 Hz, surface area of the working electrode = 0.6 mm2 and the convection rate = 2000 rpm. Under these optimized conditions, the adsorptive stripping voltammetry (AdSV) peak current was proportional over the concentration range 5 × 10-7 - 6 × 10-6 M (r = 0.999). Recoveries for acebutolol from human plasma and urine were in the range 97-103% and 96-104% respectively. The method proved to be precise (intra-day precision expressed as %RSD in human plasma ranged from 2.9 - 3.2% and inter-day precision expressed as %RSD ranged from 3.4 - 3.8%) and accurate (intra-day accuracies expressed as % error in human urine ranged from -3.3 - 2.8% and inter-day accuracies ranged from -3.3 - 1.7%). The limit of quantitation (LOQ) and limit of detection (LOD) for acebutolol were 1.7 × 10-7 and 5 × 10-7 M, respectively. Possible interferences by substances usually present in the pharmaceutical formulations were investigated with a mean recovery of 101.6 ± 0.64%. Results of the developed square-wave adsorptive stripping voltammetry (SW-AdSV) method were comparable with those obtained by reference analytical method.

Determination of Diazepam and Clonazepam in Natural Water – a Voltammetric Study

“…Organic compounds, such as pharmaceutical compounds drugs, dyes, pesticides, flavonoids, etc. were studied with HMDE . Although voltammetric techniques employing HMDE usually have low selectivity, high sensibility, good limits of detection, no or low sample preparation time, simple analysis and low costs, which are considerable advantages.…”

Eco‐friendly synthesis of gelatin‐capped bimetallic Au–Ag nanoparticles for chemiluminescence detection of anticancer raloxifene hydrochloride

“…The spiking technique was employed to detect the tested drug, RLX, in human serum and urine. One milliliter of human serum was first spiked with different drug concentrations and the samples were then deproteinated by a precipitation process according to a previously reported method . Healthy volunteers provided the human urine samples and 5.0 mL of urine was spiked with accurate amounts of the tested drug, no further treatment of samples was required after dilution.…”

Application of silver nanoparticles to the chemiluminescence determination of cefditoren pivoxil using the luminol–ferricyanide system

“…One mL of serum was spiked with CTP standard drug solution to achieve 1.8 µg/mL and deprotonated by adding 1.0 mL acetonitrile. Then 0.1 mL of NaOH (0.1 mol/L), 1.0 mL of ZnSO 4 .7H 2 O (5.0% w/v) were added, where most of the interfering species (mainly proteins) were removed by precipitation .…”