2010
DOI: 10.1016/j.anaerobe.2009.04.003
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Development of strain-specific PCR primers for the identification of Fusobacterium nucleatum subsp. fusiforme ATCC 51190T and subsp. vincentii ATCC 49256T

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Cited by 9 publications
(10 citation statements)
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“…While all three kits were accurate without exception, the strain was not detected using the PCR primers. These results underscore the primers’ high specificity for the subspecies fusiforme , as indicated in the original publications [27]. Jervøe-Storm et al [8] and Verner et al [52] showed that, compared with the cultivation method, detection reliability using real-time PCR varies from pathogen to pathogen.…”
Section: Discussionsupporting
confidence: 60%
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“…While all three kits were accurate without exception, the strain was not detected using the PCR primers. These results underscore the primers’ high specificity for the subspecies fusiforme , as indicated in the original publications [27]. Jervøe-Storm et al [8] and Verner et al [52] showed that, compared with the cultivation method, detection reliability using real-time PCR varies from pathogen to pathogen.…”
Section: Discussionsupporting
confidence: 60%
“…vincentii not available (208)Shin et al [27]Fv35-R1CCC AAG GAA AAT ACT AAFs17-F14GAT GAG GAT GAA AAG AAA CAA AGT A Fusobacterium nucleatum subsp. fusiforme not available (393)Shin et al [27]Fs17-R14CCA TTG AGA AGG GCT ATT GACPrInfw a TTT GTT GGG GAG TAA AGC GGG Prevotella intermedia 458-1,032 (404)Ashimoto et al [25]PrInrev a TCA ACA TCT CTG TAT CCT GCG TAgAcfw a AAA CCC ATC TCT GAG TTC TTC TTC Aggregatibacter actinomycetemcomitans 478-1,034 (557)Ashimoto et al [25]AgAcrev a ATG CCA ACT TGA CGT TAA ATEiCofw a CGA TTA GCT GTT GGG CAA CTT Eikenella corrodens not available (410)Furcht et al [7]EiCorev a ACC CTC TGT ACC GAC CAT TGT ATCaRefw a TTT CGG AGC GTA AAC TCC TTT TC Campylobacter rectus 415-1,012 (598)Slots et al [24]CaRerev a TTT CTG CAA GCA GAC ACT CTTSm479FTCG CGA AAA AGA TAA ACA AAC A Streptococcus mutans 599-1,077 (478)Chen et al [26]Sm479RGCC CCT TCA CAG TTG GTT AGLF1AAT ACC GCA TTA CAA CTT TG Lactobacillus fermentum 196-529 (337)(Dickson et al, 2005)LF2GGT TAA ATA CCG TCA ACG TA a Names assigned in this study …”
Section: Methodsmentioning
confidence: 99%
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“…Initially, all clinical isolates were confirmed as F. nucleatum by PCR (5) and RAPID32A (bioMérieux, France). To determine which subspecies we were working with, we used specific PCR primers described in the literature (17,29) to identify F. nucleatum subsp. vincentii and F. nucleatum subsp.…”
Section: Methodsmentioning
confidence: 99%
“…The melting curve analysis showed that non-specific PCR products were not amplified (data not shown). According to the recommendation for determining the optimal annealing temperature (OAT) for PCR (Shin et al, 2010), gradient PCR was performed with the genomic DNAs of P. gingivalis ATCC 33277 T and P. endodontalis ATCC 35406 T (data not shown). According to the PrimerSelect program, the recommended OAT was 55.7°C but according to the gradient PCR data, the Pg-F/Pg-R primers can detect P. gingivalis up to 72°C while maintaining the signal intensity of the amplicons.…”
mentioning
confidence: 99%