Development of strains of the thermotolerant yeast Hansenula polymorpha capable of alcoholic fermentation of starch and xylan

Voronovsky, Andriy Y.; Rohulya, Olha V.; Abbas, Charles A.; Sibirny, Andriy А.

doi:10.1016/j.ymben.2009.04.001

Cited by 71 publications

(24 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By overexpressing the gene PDC1 encoding pyruvate decarboxylase (PDC) in the strain 2EthOH -, the ethanol production reached 2.5 g/l at 48°C. Voronovsky et al (2009) confirmed the potentials of this yeast for biomass conversion, and the new strain could ferment starch and xylan. They co-expressed T. reesei xyn11B (encoding an endoxylanase) and A. niger xlnD (encoding β-xylosidase) in H. polymorpha under control of glyceraldehyde-3-phosphate dehydrogenase gene promoter.…”

Section: Hansenula Polymorphasupporting

confidence: 63%

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

2015

View full text Add to dashboard Cite

HIGHLIGHTS Various CBP strategies have been discussed. Recently, lignocellulosic biomass as the most abundant renewable resource has been widely considered for bioalcohols production. However, the complex structure of lignocelluloses requires a multi-step process which is costly and time consuming. Although, several bioprocessing approaches have been developed for pretreatment, saccharification and fermentation, bioalcohols production from lignocelluloses is still limited because of the economic infeasibility of these technologies. This cost constraint could be overcome by designing and constructing robust cellulolytic and bioalcohols producing microbes and by using them in a consolidated bioprocessing (CBP) system. This paper comprehensively reviews potentials, recent advances and challenges faced in CBP systems for efficient bioalcohols (ethanol and butanol) production from lignocellulosic and starchy biomass. The CBP strategies include using native single strains with cellulytic and alcohol production activities, microbial co-cultures containing both cellulytic and ethanologenic microorganisms, and genetic engineering of cellulytic microorganisms to be alcohol-producing or alcohol producing microorganisms to be cellulytic. Moreover, high-throughput techniques, such as metagenomics, metatranscriptomics, next generation sequencing and synthetic biology developed to explore novel microorganisms and powerful enzymes with high activity, thermostability and pH stability are also discussed. Currently, the CBP technology is in its infant stage, and ideal microorganisms and/or conditions at industrial scale are yet to be introduced. So, it is essential to bring into attention all barriers faced and take advantage of all the experiences gained to achieve a high-yield and low-cost CBP process.

show abstract

Section: Hansenula Polymorphasupporting

confidence: 63%

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

2015

View full text Add to dashboard Cite

show abstract

“…For the efficient utilization of xylose and starch, amylolytic and xylanolytic enzymes were heterologously expressed. Genes encoding α amylase and glucoamylase, SWA2 and GAM1, from the yeast Schwanniomyces occidentalis, encoding α-amylase and glucoamylase, were transferred in to H. polymorpha under the well characterized constitutive promoter of the H. polymorpha glyceraldehyde-3-phosphate dehydrogenase gene (Voronovsky et al, 2009). Since the organism is highly thermotolerant, engineering the substrate utilization strategies will help to improve the industrial production.…”

Section: Substrate Utilization Of Non-conventional Yeastsmentioning

confidence: 99%

Synthetic Biology and Metabolic Engineering Approaches and Its Impact on Non-Conventional Yeast and Biofuel Production

et al. 2017

View full text Add to dashboard Cite

The increasing fossil fuel scarcity has led to an urgent need to develop alternative fuels. Currently microorganisms have been extensively used for the production of first-generation biofuels from lignocellulosic biomass. Yeast is the efficient producer of bioethanol among all existing biofuels option. Tools of synthetic biology have revolutionized the field of microbial cell factories especially in the case of ethanol and fatty acid production. Most of the synthetic biology tools have been developed for the industrial workhorse Saccharomyces cerevisiae. The non-conventional yeast systems have several beneficial traits like ethanol tolerance, thermotolerance, inhibitor tolerance, genetic diversity, etc., and synthetic biology have the power to expand these traits. Currently, synthetic biology is slowly widening to the non-conventional yeasts like Hansenula polymorpha, Kluyveromyces lactis, Pichia pastoris, and Yarrowia lipolytica. Herein, we review the basic synthetic biology tools that can apply to non-conventional yeasts. Furthermore, we discuss the recent advances employed to develop efficient biofuel-producing non-conventional yeast strains by metabolic engineering and synthetic biology with recent examples. Looking forward, future synthetic engineering tools' development and application should focus on unexplored non-conventional yeast species.

show abstract

“…Therefore, the addition of the cellulolytic traits into fermentable yeast has been of interest to scientists. The heterologous expression of cellulases and hemicellulases has been primarily studied in bacterial hosts such as engineered Escherichia coli (Ryu and Karim 2011), Klebsiella oxytoca (Zhou and Ingram 2001), and Zymomonas mobilis (Vasan et al 2011) and in the yeasts Saccharomyces cerevisiae (reviewed in Hasunuma and Kondo 2012), Hansenula polymorpha (Voronovsky et al 2009), and Kluyveromyces marxianus (Yanase et al 2010a). The direct ethanol fermentation from phosphoric acid swollen cellulose (PASC) using cellulase-coexpressing yeast, which produces endoglucanases, cellobiohydrolases, and -glucosidases, was reported.…”

Section: Cbp Fermentation Of High-concentration Uhkpmentioning

confidence: 99%

Direct Fungal Production of Ethanol from High-Solids Pulps by the Ethanol-fermenting White-rot Fungus Phlebia sp. MG-60

Kamei¹,

Hirota²,

Meguro³

2014

BioResources

View full text Add to dashboard Cite

A white-rot fungus, Phlebia sp. MG-60, was applied to the fermentation of high-solid loadings of unbleached hardwood kraft pulp (UHKP) without the addition of commercial cellulase. From 4.7% UHKP, 19.6 g L -1 ethanol was produced, equivalent to 61.7% of the theoretical maximum. The highest ethanol concentration (25.9 g L -1 , or 46.7% of the theoretical maximum) was observed in the culture containing 9.1% UHKP. The highest filter paper activity (FPase) was observed in the culture containing 4.7% UHKP, while the production of FPase in the 16.5% UHKP culture was very low. Temporarily removing the silicone plug from Erlenmeyer flasks, which relieved the pressure and allowed a small amount of aeration, improved the yield of ethanol produced from the 9.1% UHKP, which reached as high as 37.3 g L -1 . These results indicated that production of cellulase and ensuing saccharification and fermentation by Phlebia sp. MG-60 is affected by water content and benefits from a small amount of aeration.

show abstract

Development of strains of the thermotolerant yeast Hansenula polymorpha capable of alcoholic fermentation of starch and xylan

Cited by 71 publications

References 27 publications

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

Synthetic Biology and Metabolic Engineering Approaches and Its Impact on Non-Conventional Yeast and Biofuel Production

Direct Fungal Production of Ethanol from High-Solids Pulps by the Ethanol-fermenting White-rot Fungus Phlebia sp. MG-60

Contact Info

Product

Resources

About