Development of STS markers linked to Hessian fly resistance gene H6 in wheat

Dweikat, İsmail; Zhang, W.; Ohm, H. W.

doi:10.1007/s00122-002-0946-9

Cited by 27 publications

(10 citation statements)

References 23 publications

(26 reference statements)

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“…The specific and diagnostic SSR markers closely linked to H9 identified in this study not only can assist wheat breeders in making parental selection but will also facilitate pyramiding the Hessian fly resistance genes into elite breeding lines during cultivar development. These markers, plus others already mapped (Dweikat et al. 1997, 2002, Seo et al.…”

Section: Discussionmentioning

confidence: 99%

Molecular mapping determines that Hessian fly resistance gene H9 is located on chromosome 1A of wheat

et al. 2005

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Hessian fly [Mayetiola destructor (Say)] is one of the major insect pests of wheat (Triticum aestivum L.) worldwide. Hessian fly resistance gene H9 was previously reported to condition resistance to Hessian fly biotype L that is prevalent in many wheat-growing areas of eastern USA and an RAPD marker, OPO05 1000 , linked to H9 in wheat was developed using wheat near-isogenic lines (NILs). However, markerassisted selection (MAS) with RAPD markers is not always feasible. One of the objectives in this study was to convert an RAPD marker linked to the gene H9 into a sequence characterized amplified region (SCAR) marker to facilitate MAS and to map H9 in the wheat genome. The RAPD fragment from OPO05 1000 was cloned, sequenced, and converted into a SCAR marker SOPO05 909 , whose linkage relationship with H9 was subsequently confirmed in two F 2 populations segregating for H9. Linkage analysis identified one sequence tagged site (STS) marker, STS-Pm3, and the eight microsatellite markers Xbarc263, Xcfa2153, Xpsp2999, Xgwm136, Xgdm33, Xcnl76, Xcnl117 and Xwmc24 near the H9 locus on the distal region of the short arm of chromosome 1A, contrary to the previously reported location of H9 on chromosome 5A. Locus Xbarc263 was 1.2 cM distal to H9, which itself was 1.7 cM proximal to loci Xcfa2153, Xpsp2999 and Xgwm136. The loci Xgwm136, Xcfa2153 and SOPO05 909 were shown to be specific to H9 and not diagnostic to several other Hessian fly resistance genes, and therefore should be useful for pyramiding H9 with other Hessian fly resistance genes in a single genotype.

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Section: Discussionmentioning

confidence: 99%

Molecular mapping determines that Hessian fly resistance gene H9 is located on chromosome 1A of wheat

et al. 2005

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“…Thus, the induction of an HR is not a requirement of an R gene. R genes are sometimes thought of as antimicrobial but genes such as tomato Mi-1 or wheat H6 confer race-specific resistance against nematodes and insects rather than microorganisms (51,147). Single R genes are generally thought to function against a single type of pathogen, but Mi-1 genes function against multiple species of root-knot nematode and aphids.…”

Section: Are There Other Types Of R Genes? What Defines An R Gene?mentioning

confidence: 99%

Elicitors, Effectors, and R Genes: The New Paradigm and a Lifetime Supply of Questions

Bent

Mackey

2007

Annu. Rev. Phytopathol.

684

548

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The plant basal immune system can detect broadly present microbe-associated molecular patterns (MAMPs, also called PAMPs) and induce defenses, but adapted microbes express a suite of effector proteins that often act to suppress these defenses. Plants have evolved other receptors (R proteins) that detect these pathogen effectors and activate strong defenses. Pathogens can subsequently alter or delete their recognized effectors to avoid defense elicitation, at risk of a fitness cost associated with loss of those effectors. Significant research progress is revealing, among other things, mechanisms of MAMP perception, the host defense processes and specific host proteins that pathogen effectors target, the mechanisms of R protein activation, and the ways in which pathogen effector suites and R genes evolve. These findings carry practical ramifications for resistance durability and for future resistance engineering. The present review uses numerous questions to help clarify what we know and to identify areas that are ripe for further investigation.

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“…Talbert et al (1994) successfully converted RFLP probes of wheat into STS markers. Later, many laboratories attempted to generate STS primers from either wheat genomic clones (Feuillet et al 1995;Talbert et al 1996;Seyfarth et al 1999;Ma et al 2004 ), RAPD PCR products (Hu et al 1997;Naik et al 1998;Dweikat et al 2002) or AFLP bands (Parker and Langridge 2000;Prins et al 2001;Smith et al 2002). The conversion of heterologous probes, such as barley clones or oat clones, is more difficult because sequence polymorphisms may exist between the homoeologous loci of wheat and barley or oat.…”

Section: Discussionmentioning

confidence: 98%

STS markers for powdery mildew resistance gene Pm6 in wheat

Qin

Wang

et al. 2007

Euphytica

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The powdery mildew resistance gene Pm6, transferred to common wheat from the tetraploid Triticum timopheevii, is effective in most epidemic areas for powdery mildew in China. RFLP probe BCD135 was previously associated with Pm6. In the present research, four STS primers (NAU/ STS BCD135-1 , NAU/STS BCD135-2 , STS003 and STS004) were designed from the sequence data of BCD135. These primers were used for PCR amplification using the genomic DNA of resistant nearisogenic lines with Pm6 and their recurrent parent, cv. Prins. No polymorphic product was observed using primers STS003 and STS004; however, primers NAU/STS BCD135-1 and NAU/STS BCD135-2 amplified two and one bands, respectively, polymorphic between the resistant near-isogenic-lines and Prins. The two primers were then used to amplify the F 2 population from the cross IGV1-465 (FAO163b/ 7*Prins) · Prins. The amplification and the powdery mildew resistance identification data were analyzed using the software Mapmaker 3.0. The results indicated that both NAU/STS BCD135-1 and NAU/ STS BCD135-2 were closely linked to Pm6 with a genetic distance of 0.8 cM. A total of 175 commercial varieties without Pm6 from different ecological areas of China were tested using marker NAU/STS BCD135-2 and none of them amplified the 230 bp-specific band. This marker thus has high practicability and can be used in MAS of Pm6 in wheat breeding programs for powdery mildew resistance.

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Development of STS markers linked to Hessian fly resistance gene H6 in wheat

Cited by 27 publications

References 23 publications

Molecular mapping determines that Hessian fly resistance gene H9 is located on chromosome 1A of wheat

Molecular mapping determines that Hessian fly resistance gene H9 is located on chromosome 1A of wheat

Elicitors, Effectors, and R Genes: The New Paradigm and a Lifetime Supply of Questions

STS markers for powdery mildew resistance gene Pm6 in wheat

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