Development of substance P, Leu-enkephalin and serotonin profiles in the lateral geniculate nuclear complex of albino rat

Wadhwa, Shashi; Rath, Sadananda; Jotwani, Geeta; Bijlani, Veena

doi:10.1016/0304-3940(90)90024-4

Cited by 9 publications

(4 citation statements)

References 19 publications

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“…The functional significance of the PACAPergic input from the RGCs to the ENK neurons also remains to be clarified. Since the ENK-containing neurons in the rat IGL project to the opposite IGL (Card and Moore 1989;Mantyh and Kemp 1983;Moore and Card 1994;Wadhwa et al 1990), they could be involved in functional integration of the IGL response during illumination.…”

Section: Discussionmentioning

confidence: 99%

“…Many of the GABAcontaining neurons store either NPY or enkephalin (ENK; Moore and Card 1994;Ohara et al 1983). In the rat, the ENK-containing neurons project to the opposite IGL (Card and Moore 1989;Mantyh and Kemp 1983;Moore and Card 1994;Wadhwa et al 1990) via the optic chiasma and posterior commissure (Mikkelsen 1992). Finally, the rat possesses a fourth unidentified cell type, which, like the GABA and/or NPY neurons, projects in the GHT (Moore and Card 1994).…”

Section: Introductionmentioning

confidence: 99%

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Photic induction of c-Fos in enkephalin neurons of the rat intergeniculate leaflet innervated by retinal PACAP fibres

2007

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The brain's biological clock, located in the suprachiasmatic nucleus (SCN), is synchronised with the cyclic environment by photic and non-photic cues. Photic information to the SCN is mediated by pituitary adenylate-cyclase-activating polypeptide (PACAP)-containing retinal ganglion cells (RGCs), whereas non-photic input originates primarily from neuropeptide Y (NPY) cells in the ipsilateral thalamic intergeniculate leaflet (IGL). RGCs also seem to project to the IGL, indicating a role for this structure in the integration of photic and non-photic inputs related to the resetting of the biological clock. In the present study, we have used anterograde tracing from both eyes, bilateral eye enucleation, double-immunofluorescence histochemistry, high-resolution confocal laser scanning microscopy and three-dimensional computer analysis to show that (1) PACAP-containing RGCs project to the IGL and are the only source for the PACAP-immunoreactive fibres in the IGL; (2) a few NPY-containing neurons in the IGL are innervated by PACAP-containing retinal nerve fibres and the contacts are both axodendritic and axosomatic; (3) most enkephalin-immunoreactive neurons in the IGL are innervated by PACAP-containing retinal afferents and the contacts are mainly axodendritic; (4) light stimulation at various time points activates (as evidenced by c-Fos induction) enkephalin-positive neurons but not NPY-immunoreactive neurons. The findings suggest that PACAP-immunoreactive retinal afferents in the IGL primarily innervate enkephalin-immunoactive neurons and that the enkephalin-containing neurons, which project locally and to the contralateral IGL, are activated by light independent of diurnal time.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Photic induction of c-Fos in enkephalin neurons of the rat intergeniculate leaflet innervated by retinal PACAP fibres

2007

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“…Despite the relative abundance of information concerning the localization of various neurotransmitters in the LGN of the adult rat, little is known about their development (see Cavanagh et al, 1991 for review). Information about the maturation of the serotonergic system during embryonic (Lidov and Molliver, 1982;Wallace and Lauder, 1983) and postnatal life (Wadhwa et al, 1990) is very limited. Here we used immunocytochemistry at the light and electron microscopic levels with anti-5-HT antibodies in the developing LGN of the rat with the aim to 1) describe how the pattern of 5-HT innervation of the LGN is established during development; 2 ) describe how the ultrastructural features of 5-HT synaptic contacts change during the first few postnatal weeks; and 3) provide a comparison of the development of the 5-HT system in the LGN with the pattern of development of this system already described in other areas of the brain.…”

mentioning

confidence: 99%

Serotonergic innervation of the lateral geniculate nucleus of the rat during postnatal development: A light and electron microscopic immunocytochemical analysis

Dinopoulos

Dori

Parnavelas

1995

J of Comparative Neurology

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The serotonergic innervation of the developing lateral geniculate nucleus of the rat was studied with immunocytochemical techniques at the light and electron microscope levels. A relatively small number of thick serotonergic fibers were observed at the time of birth, distributed more densely in the ventral portion of the nucleus and in the intergeniculate leaflet than in the dorsal lateral geniculate nucleus. By the end of the first postnatal week, this distribution pattern was more clearly established, but the number of immunoreactive fibers was increased. Thereafter, and until the adult pattern was established at the end of the third postnatal week, serotonergic fibers increased further in number and changed morphologically (e.g., they became finer and more ramified with closely spaced varicosities), but their pattern of distribution remained unchanged. Electron microscopical analysis of the dorsal lateral geniculate nucleus revealed that the vast majority of serotonin varicosities formed asymmetrical synapses with dendritic shafts; axosomatic synapses were a feature of the nucleus only at the time of birth. The proportion of serotonin varicosities forming synapses increased gradually from birth to reach a peak at the end of the second postnatal week, then declined markedly in the following week before increasing again at a later stage. It may be speculated that synapses formed during the first two weeks of life may be related to the involvement of serotonin in the morphogenesis of the lateral geniculate nucleus, whereas those formed later in development may be involved in the mediation of neurotransmitter effects.

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“…Substance-P (SP)-immunoreactive neurons and axons have been described in many areas of the rat central nervous system including a number of visual structures (Cuello et al, 1978;Ljungdahl et al, 1978). Substance-P-immunoreactive axons have been observed in the lateral geniculate nucleus (Brecha et al, 1987;Wadhwa et al, 1990), the pregeniculate nucleus and the pretectal olivary nucleus (Nakagawa et al, 1988), the parabigeminal nucleus (Bennett-Clarke et al, 1989), and the pulvinar LP complex (Hutsler & Chalupa, 1991). Substance-P-immunoreactive axons have been observed in the lateral geniculate nucleus (Brecha et al, 1987;Wadhwa et al, 1990), the pregeniculate nucleus and the pretectal olivary nucleus (Nakagawa et al, 1988), the parabigeminal nucleus (Bennett-Clarke et al, 1989), and the pulvinar LP complex (Hutsler & Chalupa, 1991).…”

Section: Introductionmentioning

confidence: 99%

Postnatal development of substance-P immunoreactivity in the rat superior colliculus

1993

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Immunocytochemical techniques have been used to examine the distribution of substance-P (SP)-labeled neurons in the superior colliculus of rats from birth to adulthood. At birth, there are almost no SP-immunopositive neurons in the tectum. A small number of SP neurons appear over the next several days. However, the vast majority of SP neurons appear between P9 and P10, and by P12 have attained adult-like numbers and distribution. Neurons are confined to the superficial layers of the colliculus, specifically the upper two-thirds of the stratum griseum superficiale (SGS). There is no indication of a differential developmental sequence along rostrocaudal or mediolateral axes. Neuronal types can be distinguished as early as P6 and include horizontal, vertical, and multipolar cells.Substance-P-immunoreactive axons and boutons are also present in the superior colliculus at birth, and are for the most part confined to the deep layers. Boutons are generally of the en-passant type. The density of labeled axons and boutons increases progressively, and by P10 there is an almost adult-like lamination and patchiness. In the adult, labeled axons and boutons are most dense in the stratum opticum and stratum griseum intermedium. Bridges of dorsoventrally oriented labeled axons span the relatively label-poor stratum album intermedium. SP label in the stratum griseum profundum is dense and patchy, and there is also dense label in the stratum album profundum bordering the periaqueductal grey.The role of substance-P-labeled neurons in the superior colliculus is still a matter of speculation. The findings of this study indicate that SP neurons may play a role in intrinsic collicular circuitry.

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Development of substance P, Leu-enkephalin and serotonin profiles in the lateral geniculate nuclear complex of albino rat

Cited by 9 publications

References 19 publications

Photic induction of c-Fos in enkephalin neurons of the rat intergeniculate leaflet innervated by retinal PACAP fibres

Photic induction of c-Fos in enkephalin neurons of the rat intergeniculate leaflet innervated by retinal PACAP fibres

Serotonergic innervation of the lateral geniculate nucleus of the rat during postnatal development: A light and electron microscopic immunocytochemical analysis

Postnatal development of substance-P immunoreactivity in the rat superior colliculus

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