Development of suitable external quality control material for G6PD deficiency screening with the fluorescent spot test

Efficiency of lyophilized hemolysate control material in proficiency testing program for G6PD deficiency screening in Thailand

“…According to the preparation of lyophilized hemolysate, packed red cells were massively diluted (1:57, v/v) with 5% sucrose solution. 6 Therefore, the G6PD activity was relatively reduced in our study. Thus, the materials with intermediate levels of G6PD activity showed the intermediate to normal…”

“…5 We previously reported that the lyophilized hemolysate materials (hereinafter called "materials") prepared from normal and G6PD deficient red blood cells are very stable and are considered as potential quality control materials for FST. 6 To broaden the usability of our materials for establishing the proficiency testing (PT) program of G6PD deficiency screening in Thailand, we distributed the materials to 59 independent laboratories including public and private sectors from different parts of Thailand as blinded samples for G6PD deficiency screening during January-June 2021. The results were generated by different screening methods and sent back to our center for further evaluation.…”

“…The materials were prepared according to protocols reported in the previous studies. 6,7 G6PD status of the materials was also tested by FST before storing at −20°C.…”