Development of surface-attached thin film of non-fouling hydrogel from poly(2-oxazoline)

Pidhatika, Bidhari; Zhao, Nan; Rühe, Jürgen

doi:10.1007/s10965-018-1677-1

Cited by 9 publications

(8 citation statements)

References 45 publications

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“…Cell culture study and phasecontrast microscopy on hydrogel-coated plastics substrates determines the aimed dual-functional properties of the hydrogel. The following protocols were adapted from previous reports (Pidhatika et al, 2019a;Pidhatika et al, 2019b).…”

Section: Methodsmentioning

confidence: 99%

“…Hydrogel formation, immobilization, and stability study were performed following the previously published protocol (Pidhatika et al, 2019a). The polymer solution was spin-coated on top of the above-described wafer.…”

Section: Hydrogel Formation Immobilization and Stabilitymentioning

confidence: 99%

“…Different from our previous publication (Pidhatika et al, 2019b) where PDMAA was used as the biopassive platform, the presented work focuses on PEOXA as the biopassive platform. Furthermore, different from our other previous publication (Pidhatika et al, 2019a) where the potency of the PEOXA network as biopassive platform was evaluated, the presented work provides more details on the synthesis and bulk characterization of benzophenonebearing PEOXA, as well as on surface characterization of the hydrogel. Moreover, the PEOXA biopassive network was combined with laminin as a bioactive compound.…”

Section: Introductionmentioning

confidence: 98%

“…The presented work aimed at developing dualfunctional hydrogel coatings, in which poly(2-ethyl-2oxazoline) (PEOXA) (Pidhatika et al 2019a) was chosen as the hydrophilic polymer and benzophenone were chosen as the cross-linker. Laminin was then covalently grafted to the hydrophilic (and biopassive) polymer network as the bioactive moiety.…”

Section: Introductionmentioning

confidence: 99%

“…Since numerous biomedical devices are made of plastics (Czuba, 2014;Padsalgikar, 2017;Sastri, 2014a;Sastri, 2014b;Sastri, 2014c;Sastri, 2014d), the dual-functional coating was studied on poly(methylmethacrylate) (PMMA) slide substrates. The reasons behind the selection of PEOXA as the biopassive platform, and laminin as the bioactive compounds are available in our previous publications (Pidhatika et al, 2019a;Pidhatika et al, 2019b). For the crosslinker, benzophenone is classified as a non-specific cross-linker that can bind to any C-H bonds in its vicinity.…”

Section: Introductionmentioning

confidence: 99%

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The preparation of dual-functional hydrogel as the surface coating of plastics in biomedical applications

Zhao¹,

Pidhatika²

2020

MKKP

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Poly(2-ethyl-2-oxazoline) (PEOXA) is among polymers that have been reported to show anti-adhesive (bio-passive) behavior in numerous biomaterial applications. This work aimed to develop dual-functional hydrogel coatings, with PEOXA as hydrophilic polymer and benzophenone as cross-linker. PEOXA was partially hydrolyzed in acidic conditions to form PEOXA-m% EI that contains ethyleneimine (EI) groups. The EI groups were used as conjugation sites for incorporation of benzophenone (BP) molecules to PEOXA chains to form PEOXA-m% EIBP. Thin films of surface-attached polymer networks were generated from PEOXA-m% EIBP copolymers composed of PEOXA as platform and benzophenone as crosslinker units. The polymer became crosslinked through benzophenone units and forms a hydrogel as biopassive platform during irradiation with ultraviolet (UV) light. Laminin was incorporated into biopassive polymer network to allow for preparation of dual-functional hydrogel. NMR spectra indicated successful control of PEOXA partial hydrolysis and conjugation of benzophenone molecules to PEOXA chains. Ellipsometry and ATR-FTIR results showed that wavelength of UV light during C, H-insertion reaction influences stability of polymer network (hydrogel) on the substrate surface. XPS spectra verified that a stable film could be generated using suitable UV light during preparation of polymer network. Cell culture study on laminin/PEOXA-coated PMAA plastics showed dual-functional properties.

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Section: Methodsmentioning

confidence: 99%

Section: Hydrogel Formation Immobilization and Stabilitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The preparation of dual-functional hydrogel as the surface coating of plastics in biomedical applications

Zhao¹,

Pidhatika²

2020

MKKP

Self Cite

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Non‐Fouling Multi‐Azide Polyoxazoline Brush‐co‐Polymers for Sensing Applications

Komsthöft,

Bartalucci,

Tibbitt

et al. 2024

Adv Materials Inter

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One of the key parameters of an artificial biosensor is a high signal‐to‐noise ratio. This is achieved by limiting non‐specific interactions while simultaneously maximizing the targeted specific interaction. Here, it is combined non‐fouling characteristics of poly(2‐methyl‐2‐oxazoline) (PMOXA) coatings with an abundance of azide groups to create a multi‐azide containing poly(2‐methyl‐2‐oxazoline‐co‐2‐(3‐azidopropyl)‐2‐oxazoline) (PMCA) that can participate in bioorthogonal strain‐promoted azide‐alkyne cycloaddition (SPAAC) for functionalization. This functional polymer is made surface‐active using the PAcrAm™ technology to obtain well‐defined spontaneously adsorbed monolayers on gold surfaces. The resistance to non‐specific interactions is tested against full human serum (HS), analyzed via variable angle spectroscopic ellipsometry (VASE), and compared to equivalent coatings based on PMOXA and azido‐poly(ethylene glycol) (PEG‐N3). The specific interactions are investigated via VASE and quartz crystal microbalance with dissipation (QCM‐D) by immobilization of dibenzocyclooctyne‐PEG4‐biotin conjugate (DBCO‐biotin) and streptavidin. The new PMCA‐based coating shows superior resistance to non‐specific protein adhesion than equivalent coatings based on commercially available PEG‐N3 and significantly increases capacity for SPAAC. A proof of principle assay (biotin‐streptavidin/biotin‐BSA/anti‐BSA) shows improved binding for the new PMCA polymer compared with single azide PEG.

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Tribological properties of hierarchical structure artificial joints with poly acrylic acid (AA) - poly acrylamide (AAm) hydrogel and Ti6Al4V substrate

Deng

Sun

et al. 2020

J Polym Res

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Development of surface-attached thin film of non-fouling hydrogel from poly(2-oxazoline)

Cited by 9 publications

References 45 publications

The preparation of dual-functional hydrogel as the surface coating of plastics in biomedical applications

The preparation of dual-functional hydrogel as the surface coating of plastics in biomedical applications

Non‐Fouling Multi‐Azide Polyoxazoline Brush‐co‐Polymers for Sensing Applications

Tribological properties of hierarchical structure artificial joints with poly acrylic acid (AA) - poly acrylamide (AAm) hydrogel and Ti6Al4V substrate

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