Development of surface plasmon resonance imaging for detection of Acidovorax avenae subsp. citrulli (Aac) using specific monoclonal antibody

Puttharugsa, Chokchai; Wangkam, Thidarat; Huangkamhang, Nongluck; Gajanandana, Oraprapai; Himananto, Orawan; Sutapun, Boonsong; Amarit, Ratthasart; Somboonkaew, Armote; Srikhirin, Toemsak

doi:10.1016/j.bios.2010.10.007

Cited by 37 publications

(30 citation statements)

References 13 publications

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“…Interestingly, in one report where the sandwich format gave a more sensitive LOD than a direct assay, the authors suggested that portion of the secondary antibody used can bind to the bacterial cells in areas that are within the penetration depth of the evanescent wavelengths especially near the area of the capture antibody. The authors gave this as the reason for the observed improvement of LOD in the sandwich assay seen in a particular study [25]. Direct detection format shows a higher LOD value compared to a sandwich or nanoparticles enhanced formats.…”

Section: Sandwich Assaymentioning

confidence: 89%

“…Several researchers have reported an improvement of LOD [25] or an improvement of the detection range in a sandwich format compared to a direct format [26]. Hence, the detection sensitivity of the assay using a sandwich approach was investigated.…”

Section: Sandwich Assaymentioning

confidence: 99%

“…On the other hand, the development of a sandwich assay format for SPR demonstrates mixed results with several researchers reporting no increase in LOD using a direct or sandwich format [28]. While several researchers reported either an improvement of LOD [25], as similarly reported in this work, or improvement of the detection range with the transition from a direct into a sandwich format [26]. Other researchers, who used SPR for assaying bacterial pathogens, only report a detection in a direct format without reporting on a sandwich format, and all mentioned on the limited penetration depth of the evanescent wave as a problem for sensitive detection of bacterial pathogen in SPR-based assay methods [12,20].…”

Section: Sandwich Assaymentioning

confidence: 99%

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Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

2017

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Abstract:Campylobacteriosis is an internationally important foodborne disease caused by Campylobacter jejuni. The bacterium is prevalent in chicken meat and it is estimated that as much as 90% of chicken meat on the market may be contaminated with the bacterium. The current gold standard for the detection of C. jejuni is the culturing method, which takes at least 48 h to confirm the presence of the bacterium. Hence, the aim of this work was to investigate the development of a Surface Plasmon Resonance (SPR) sensor platform for C. jejuni detection. Bacterial strains were cultivated in-house and used in the development of the sensor. SPR sensor chips were first functionalized with polyclonal antibodies raised against C. jejuni using covalent attachment. The gold chips were then applied for the direct detection of C. jejuni. The assay conditions were then optimized and the sensor used for C. jejuni detection, achieving a detection limit of 8 × 10 6 CFU·mL −1 . The sensitivity of the assay was further enhanced to 4 × 10 4 CFU·mL −1 through the deployment of a sandwich assay format using the same polyclonal antibody. The LOD obtained in the sandwich assay was higher than that achieved using commercial enzyme-linked immunosorbent assay (ELISA) (10 6 -10 7 CFU·mL −1 ). This indicate that the SPR-based sandwich sensor method has an excellent potential to replace ELISA tests for C. jejuni detection. Specificity studies performed with Gram-positive and Gram-negative bacteria, demonstrated the high specific of the sensor for C. jejuni.

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Section: Sandwich Assaymentioning

confidence: 89%

Section: Sandwich Assaymentioning

confidence: 99%

Section: Sandwich Assaymentioning

confidence: 99%

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Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

2017

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“…[11][12][13][14][15][16][17][18][19][20][21] The first type of SPR sensor is a so-called SPR imaging sensor, where a 2-dimension CCD sensor is used for measuring reflected light from the sensor chip, on which a multi-channel or micro array was fabricated. [11][12][13][14] For example, Piliarik et al 11 reported a high-throughput SPR imaging sensor for nucleic acids, while identifying a specific bacteria pathogenous. The sensitivity resolution of the sensor was 2 × 10 -7 RIU, and the detection of nucleic acid at the 100 pM level was achieved.…”

Section: Introductionmentioning

confidence: 99%

“…In this case, 120 measuring areas were successfully fabricated on a sensor chip with 6 flow channels and 20 sensing points in each flow channel. In addition, Chinowsky et al, 12 and Puttharugsa et al 13 also developed potable SPR imaging sensors based on a prism-coupled SPR sensor system with multi detection areas. They applied the SPR imaging sensor to detect bio-compounds, such as viruses and microbes.…”

Section: Introductionmentioning

confidence: 99%

Development of a Portable Surface Plasmon Resonance Sensor with Multi-Sensing Points Based on the Linear CCD Sensor

et al. 2016

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A portable-type surface plasmon resonance (SPR) sensor, composed from a new optical system for multi-sensing, has been developed to apply to environment analysis, clinical diagnosis etc., where many samples are desired to be analyzed at high throughput. The optical system of the sensor consists of a light-emitting diode, a pair of cylindrical lenses, a pair of collimator lenses, a correction lens, a prism, a polarizer and a linear CCD sensor with 2048 pixels. Reflected light from a sensor chip of the width of 6 mm at a certain incident angle was detected by ca. 618 pixels of the linear CCD sensor as an SPR sensor signal. An SPR sensor signal at a specified incident angle is controllable for optimization by adjusting the position of the CCD sensor. A sensor chip having a 30-stripe linear pattern (100 μm width/stripe) was prepared. The spatial resolution as well as the performance of the sensor were evaluated by using sucrose solutions. As a result, the acquisition of SPR sensor signals from 30 sensing points was successfully achieved with a spatial resolution of 100 μm (distance between 2 sensing points). A lower detection limit of ca. 3.2 -5.5 × 10 -5 RIU with a standard deviation of ±4.5% was obtained by averaging the signals from 6 -7 pixels of the CCD sensor per one sensing stripe.

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Particle Assays

Chen

2023

Lecture Notes in Chemistry

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Development of surface plasmon resonance imaging for detection of Acidovorax avenae subsp. citrulli (Aac) using specific monoclonal antibody

Cited by 37 publications

References 13 publications

Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

Surface Plasmon Resonance Immunosensor for the Detection of Campylobacter jejuni

Development of a Portable Surface Plasmon Resonance Sensor with Multi-Sensing Points Based on the Linear CCD Sensor

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